Facile Synthesis of Triangular and Hexagonal Anionic Gold Nanoparticles and Evaluation of Their Cytotoxicity

Tripathi, Ravi Mani; Yoon, Sun‐Young; Ahn, Do-Hee; Chung, Sang J.

doi:10.3390/nano9121774

Cited by 19 publications

(14 citation statements)

References 32 publications

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“…Figure S2 show the FTIR spectrum of biosynthesized nanosheets in the scanning range of 650 to 4000 cm −1 . A broad and strong peak was observed at 3448.1 cm −1 , corresponding to the -OH stretching vibrations of the OH units and water [34]. A medium peak was observed at 2973.7 cm −1 , which corresponded to C-H stretching vibrations of alkane.…”

Section: Ftir Analysismentioning

confidence: 97%

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

et al. 2020

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Recent developments in the area of nanotechnology have focused on the development of nanomaterials with catalytic activities. The enzyme mimics, nanozymes, work efficiently in extreme pH and temperature conditions, and exhibit resistance to protease digestion, in contrast to enzymes. We developed an environment-friendly, cost-effective, and facile biological method for the synthesis of ZnO-Pd nanosheets. This is the first biosynthesis of ZnO-Pd nanosheets. The synthesized nanosheets were characterized by UV–visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray. The d-spacing (inter-atomic spacing) of the palladium nanoparticles in the ZnO sheets was found to be 0.22 nm, which corresponds to the (111) plane. The XRD pattern revealed that the 2θ values of 21.8°, 33.3°, 47.7°, and 56.2° corresponded with the crystal planes of (100), (002), (112), and (201), respectively. The nanosheets were validated to possess peroxidase mimetic activity, which oxidized the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate in the presence of H2O2. After 20 min of incubation time, the colorless TMB substrate oxidized into a dark-blue-colored one and a strong peak was observed at 650 nm. The initial velocities of Pd-ZnO-catalyzed TMB oxidation by H2O2 were analyzed by Michaelis–Menten and Lineweaver–Burk plots, resulting in 64 × 10−6 M, 8.72 × 10−9 Msec−1, and 8.72 × 10−4 sec−1 of KM, Vmax, and kcat, respectively.

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Section: Ftir Analysismentioning

confidence: 97%

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

et al. 2020

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“…We previously developed an ultrasensitive and selective colorimetric detection method for lead [14]. We previously reported the biosynthesis of triangular and hexagonal gold nanoparticles [15], palladium nanoparticles [16][17][18], and HAp nanofibers [19]. The present study reports the biosynthesis of nanoclusters.…”

Section: Introductionmentioning

confidence: 72%

Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species

Tripathi

Chung

2020

Molecules

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Hydrogen peroxide is a low-reactivity reactive oxygen species (ROS); however, it can easily penetrate cell membranes and produce highly reactive hydroxyl radical species through Fenton’s reaction. Its presence in abnormal amounts can lead to serious diseases in humans. Although the development of a simple, ultrasensitive, and selective method for H2O2 detection is crucial, this remains a strategic challenge. The peroxidase mimetic activity of palladium nanoclusters (PdNCs) has not previously been evaluated. In this study, we developed an ultrasensitive and selective colorimetric detection method for H2O2 using PdNCs. An unprecedented eco-friendly, cost-effective, and facile biological method was developed for the synthesis of PdNCs. This is the first report of the biosynthesis of PdNCs. The synthesized nanoclusters had a significantly narrow size distribution profile and high stability. The nanoclusters were demonstrated to possess a peroxidase mimetic activity that could oxidize peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Various interfering substances in serum (100 μM phenylalanine, cysteine, tryptophan, arginine, glucose, urea, Na+, Fe2+, PO43−, Mn+2, Ca2+, Mg2+, Zn2+, NH4+, and K+) were included to evaluate the selectivity of the assay, and oxidation of TMB occurred only in the presence of H2O2. Therefore, PdNCs show an efficient nanozyme for the peroxidase mimetic activity. The assay produced a sufficient signal at the ultralow concentration of 0.0625 µM H2O2. This colorimetric assay provides a real-time, rapid, and easy-to-use platform for the detection of H2O2 for clinical purposes.

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“…The zeta potential was obtained at 11.1 mV z-deviation and 2.26 mS/cm conductivity. The negative potential revealed the presence of biological moieties on the surface of nanocomposites [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…The biosynthesized SnO 2 -Cu nanocomposites were scanned from 650 to 4000 cm −1 ( Figure S2 ). The FTIR spectrum showed a strong and broad peak at 3330 cm −1 , corresponding to the –OH stretching vibrations of the OH units and water [ 24 ]. A strong peak was observed at 1640 cm −1 , indicating the bond for (N–H) bending, which corresponds to primary amines [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…Platycladus species are enriched sources of carbohydrates, alkaloids, glycosides, flavonoids, tannins, and saponins [ 23 ]. These biomolecules are important sources for the biosynthesis of nanomaterials [ 24 , 25 , 26 ]. Platycladus species extracts have already been used for the synthesis of copper nanoparticles and reduced graphene oxide [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Eco-Friendly Synthesis of SnO2-Cu Nanocomposites and Evaluation of Their Peroxidase Mimetic Activity

Tripathi

Chung

2021

Nanomaterials

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The enzyme mimetic activity of nanomaterials has been applied in colorimetric assays and point-of-care diagnostics. Several nanomaterials have been exploited for their peroxidase mimetic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. However, an efficient nanomaterial for the rapid and strong oxidation of TMB remains a strategic challenge. Therefore, in this study, we developed copper-loaded tin oxide (SnO2-Cu) nanocomposites that rapidly oxidize TMB. These nanocomposites have strong absorption at 650 nm and can be used for highly sensitive colorimetric detection. An environmentally friendly (green), rapid, easy, and cost-effective method was developed for the synthesis of these nanocomposites, which were characterized using ultraviolet-visible, energy-dispersive X-ray, and Fourier-transform infrared spectroscopy, as well as scanning electron microscopy. This is the first green synthesis of SnO2-Cu nanocomposites. Their enzyme mimetic activity, which was first studied here, was found to be strongly dependent on the temperature and pH value of the solution. The synthesized nanocomposites have the advantages of low cost, high stability, and ease of preparation for enzyme mimetic applications. Hence, SnO2-Cu nanocomposites are a promising alternative to peroxidase enzymes in colorimetric point-of-care diagnostics.

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Facile Synthesis of Triangular and Hexagonal Anionic Gold Nanoparticles and Evaluation of Their Cytotoxicity

Cited by 19 publications

References 32 publications

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species

Eco-Friendly Synthesis of SnO2-Cu Nanocomposites and Evaluation of Their Peroxidase Mimetic Activity

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