Facilitated Diffusion of Proline Across Membranes of Liposomes and Living Cells by a Calix[4]Pyrrole Cavitand

Martínez‐Crespo, Luis; Sun‐Wang, Jia Liang; Sierra, Andrés Felipe; Aragay, Gemma; Errasti-Murugarren, Ekaitz; Bartoccioni, Paola; Palacı́n, Manuel; Ballester, Pedro J.

doi:10.2139/ssrn.3554084

“…Previous studies showed that active L-proline transport could occur in artificial proteoliposomes that were reconstituted with purified PutP [37] or ProP [38] proteins. More recent studies indicated that facilitated diffusion of L-proline could also occur with the synthetic carrier calyx [4]pyrrole in liposomes and living HeLa cells [39] . The apparent saturation kinetics for L-proline utilization by the triple mutant CDC11-1 suggested that another membrane transporter might be involved.…”

Section: Growth Of E Coli Strain Cdc11-1 With L-proline As the Nitrog...mentioning

confidence: 99%

Growth of Escherichia coli K-12 on L-proline in the absence of known proline transporters

Deutch¹

2023

J. Adv. Microbiol. Res.

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Three transport systems have been implicated in the uptake of L-proline by the Gram-negative bacterium Escherichia coli. A triple mutant containing deletions of the putP, proP, and proW genes was constructed from strain BW25113 of the Keio collection. This mutant still grew slowly in a minimal salts medium with L-proline as the sole nitrogen source. Growth was dependent on the exogenous proline concentration but was not stimulated by 0.3 mol l -1 NaCl. It was reduced by addition of 5 mmol l -1 L-isoleucine by not by L-leucine, glycine betaine, γaminobutyric acid, or sarcosine. The triple mutant was partially inhibited by L-azetidine-2-carboxylate but not by 3,4-dehydro-DL-proline. Introduction of additional mutations in the proY, brnQ, or gabP genes had no effect. While the mechanism of proline uptake in this mutant is still unclear, it is likely that another membrane transporter can facilitate L-proline accumulation when the normal systems are unavailable.

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“…Previous studies showed that active L-proline transport could occur in artificial proteoliposomes that were reconstituted with purified PutP [37] or ProP [38] proteins. More recent studies indicated that facilitated diffusion of L-proline could also occur with the synthetic carrier calyx [4]pyrrole in liposomes and living HeLa cells [39] . The apparent saturation kinetics for L-proline utilization by the triple mutant CDC11-1 suggested that another membrane transporter might be involved.…”

Section: Growth Of E Coli Strain Cdc11-1 With L-proline As the Nitrog...mentioning

confidence: 99%

Untitled

2023

J. Adv. Microbiol. Res.

0

View full text Add to dashboard Cite

Three transport systems have been implicated in the uptake of L-proline by the Gram-negative bacterium Escherichia coli. A triple mutant containing deletions of the putP, proP, and proW genes was constructed from strain BW25113 of the Keio collection. This mutant still grew slowly in a minimal salts medium with L-proline as the sole nitrogen source. Growth was dependent on the exogenous proline concentration but was not stimulated by 0.3 mol l -1 NaCl. It was reduced by addition of 5 mmol l -1 L-isoleucine by not by L-leucine, glycine betaine, γaminobutyric acid, or sarcosine. The triple mutant was partially inhibited by L-azetidine-2-carboxylate but not by 3,4-dehydro-DL-proline. Introduction of additional mutations in the proY, brnQ, or gabP genes had no effect. While the mechanism of proline uptake in this mutant is still unclear, it is likely that another membrane transporter can facilitate L-proline accumulation when the normal systems are unavailable.

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“…The research groups of Ballester and Palacin extracted some novel findings related to the involvement of C4P-based cavitands in the recognition, sensing, and selective transport of AAs across liposomal membranes and living cells. 103 It was revealed that the deep C4P-based cavitand ( 67 , Fig. 9) selectively forms a 1 : 1 host–guest complex with l -proline in the presence of various competing AAs, and hence functions as an effective carrier towards the facilitated diffusion of l -proline across liposomal membranes.…”

Section: C4p-based Systems As Sensorsmentioning

confidence: 99%