Facilitated maturation of Ca²⁺ handling properties of human embryonic stem cell-derived cardiomyocytes by calsequestrin expression

Abstract

RA. Facilitated maturation of Ca 2ϩ handling properties of human embryonic stem cell-derived cardiomyocytes by calsequestrin expression.

Proteomic Analysis of Human Pluripotent Stem Cell–Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation

“…The small overlap maybe attributed to the precipitation of plasma membrane proteins during 2D-differential-in-gel electrophoresis experiments and are, therefore, mostly excluded from our analysis 43 Gundry et al 44 examined published proteomic data on pluripotent stem cells and showed that overlap among different proteomic studies are generally small and this is consistent with what we observed. Of the ones that were found in both the studies, only 31% showed concordant changes between -handling proteins, 45 microRNAs, 46 and epigenetic components 47 that are determinants of the maturation of hESC-CMs. Led by these data and other clues, we found that induced contractions via electric stimulation increase the expression of contractile genes and improve the organization of myofilaments.…”

Determination of the Human Cardiomyocyte mRNA and miRNA Differentiation Network by Fine-Scale Profiling

“…These maturity genes include CASQ2, whose forced overexpression in fetal-like hiPSC-derived cardiomyocytes results in a more mature calcium handling phenotype [35]. Beyond CASQ2, CRYAB and DESMIN (not shown) were identified as maturation genes.…”

“…Following blasticidin selection, cultures were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum for the duration of the cultures. On days 0, 3,7,10,14,20,28,35,45,60,90, and 120, approximately 3 million cells were removed for RNA collection. The differentiation protocol and sample collection were performed in 3 independent replicates as indicated by Run 1, 2, and 3 in Figs.…”

Characterization of contracting cardiomyocyte colonies in the primary culture of neonatal rat myocardial cells: A model of in vitro cardiomyogenesis

“…A similar trend was also observed in cultured embryonic stem cells. 31,32 However, while embryonic stem cell cultures can be used successfully to investigate prenatal heart development, 33,34 the formation of contracting cardiomyocyte colonies is more relevant to postnatal cardiomyogenesis.…”