Facilitated on-line supercritical fluid extraction - supercritical fluid chromatography for nonpolar and polar compounds from milk thistle seeds

Lipidomics Study of Type 1 Diabetic Rats Using Online Phase Transition Trapping-Supercritical Fluid Extraction-Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

“…Compared with high performance liquid chromatography, SFC offers faster elution times and higher separation efficiency . Therefore, the online SFE-SFC system successfully combines the advantages of both to achieve green and efficient automated analysis. , However, it faces a significant challenge: peak broadening and deformation due to large-volume extraction . To address peak widening, Sakai et al introduced a trap column before the analytical column and used a higher split ratio to minimize peak distortion caused by the strong extractant .…”