Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch

Guo, Haoyu; Tian, Rongzhen; Wu, Yaokang; Lv, Xueqin; Li, Jianghua; Liu, Long; Du, Guocheng; Chen, Jian; Liu, Yanfeng

doi:10.1016/j.synbio.2024.04.010

Synthetic and Systems Biotechnology

2024

DOI: 10.1016/j.synbio.2024.04.010

|View full text |Cite

Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch

Haoyu Guo,

Rongzhen Tian,

Yaokang Wu

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

2025

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology

Cao,

Li,

Liu

et al. 2025

Synthetic and Systems Biotechnology

View full text Add to dashboard Cite

Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology

Cao,

Li,

Liu

et al. 2025

Synthetic and Systems Biotechnology

View full text Add to dashboard Cite

Plasmid-Stabilizing Strains for Antibiotic-Free Chemical Fermentation

Guo,

Xia,

Liang

et al. 2024

ACS Synth. Biol.

View full text Add to dashboard Cite

Plasmid-mediated antibiotic-free fermentation holds significant industrial potential. However, the requirements for host elements and energy during plasmid inheritance often cause cell burden, leading to plasmid loss and reduced production. The stable maintenance of plasmids is primarily achieved through a complex mechanism, making it challenging to rationally design plasmid-stabilizing strains and characterize the associated genetic factors. In this study, we introduced a fluorescence-based high-throughput method and successfully screened plasmid-stabilizing strains from the genomic fragment-deletion strains of Escherichia coli MG1655 and Bacillus subtilis 168. The application of EcΔ50 in antibiotic-free fermentation increased the alanine titer 2.9 times. The enhanced plasmid stability in EcΔ50 was attributed to the coordinated deletion of genes involved in plasmid segregation and replication control, leading to improved plasmid maintenance and increased plasmid copy number. The increased plasmid stability of BsΔ44 was due to the deletion of the phage SPP1 surface receptor gene yueB, resulting in minimized sporulation, improved plasmid segregational stability and host adaptation. Antibiotic-free fermentation results showed that strain BsΔyueB exhibited a 61.99% higher acetoin titer compared to strain Bs168, reaching 3.96 g/L. When used for the fermentation of the downstream product, 2,3-butanediol, strain BsΔyueB achieved an 80.63% higher titer than Bs168, reaching 14.94 g/L using rich carbon and nitrogen feedstocks. Overall, our work provided a plasmid-stabilizing chassis for E. coli and B. subtilis, highlighting their potential for antibiotic-free fermentation of valuable products and metabolic engineering applications.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch

Cited by 2 publications

References 44 publications

Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology

Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology

Plasmid-Stabilizing Strains for Antibiotic-Free Chemical Fermentation

Contact Info

Product

Resources

About