Facilitation of Gene Transfection and Cell Adhesion by Gelatin‐Functionalized PCL Film Surfaces

Abstract

Efficient local gene transfection on a tissue scaffold is of crucial importance in facilitating tissue repair and regeneration. In this work, the gelatin‐functionalized polycaprolactone (PCL) film surfaces are prepared via surface‐initiated atom transfer radical polymerization of glycidyl methacrylate. The resultant covalent attachment of gelatin could enhance the cell‐adhesion and local gene transfection properties. The gelatin‐functionalized PCL film surfaces exhibit excellent cell‐adhesion ability to both adherent and suspension cells. The attached adherent cells demonstrate the characteristic elongated morphologies with good spreading capability, while the attached suspension cells can maintain the original status of the round morphologies without spreading. More importantly, the gelatin coupled on the PCL surface could be used to absorb the cationic vector/plasmid deoxyribonucleic acid (pDNA) complexes via electrostatic interaction. The local gene transfection property on the immobilized cells is dependent on both the density of the immobilized cells and the loading types of pDNA complexes. The transfection efficiency of different assemble methods of pDNA complex was compared. With the pre‐ and post‐loading sandwich‐like gene transfection, the gelatin‐functionalized PCL film surface can substantially enhance the transfection properties to different cell lines. The present study is very useful to spatially control local gene delivery within PCL‐based tissue scaffolds.

Hydrazine-Mediated Construction of Nanocrystal Self-Assembly Materials

“…The former is assigned to ÀCOOH, and the latter is assigned to ÀCH 2 À. 59 In the self-assembly materials, a clear shift of ÀCOOH peak to 287.9 eV is observed, representing the variation of ; CdO chemical environment. 59 Because MPA is the only source of C, this result strongly supports the amidation of the ÀCOOH of MPA (Figure 4d).…”

Post-polymerization modification reactions of poly(glycidyl methacrylate)s

“…On this basis, the discussion does not exhaust the entire literature and in particular does not examine surface functionalisations and particulate materials. [17][18][19][20][21][22][23][24][25][26][27][28][29] Furthermore, cross-linked systems are not covered. [30][31][32][33][34] The synthesis of the backbone itself is not discussed and the reader is referred to a number of important publications in this regard.…”

Fibronectin-Modified Surfaces for Evaluating the Influence of Cell Adhesion on Sensitivity of Leukemic Cells to siRNA Nanoparticles

“…For the transfection studies, complexes were added on the scaffolds before or after cell seeding. The percentage of EGFP expression on transfected cells was slightly higher as compared to cells transfected on tissue culture plates: Percentage of EGFP-positive K562 cells was 7.4% and 4.7% on scaffolds and tissue culture plastic, respectively, while no transfection was observed on PCL [29]. The gene expression was not very efficient with this carrier, but no adverse effect of cell attachment on transfection was seen.…”

“…Yuan, et al modified polycaprolactone (PCL) films via surface-initiated atom transfer radical polymerization of poly(glycidyl methacrylate) (PGMA) to covalently immobilize gelatin [29]. The authors used these scaffolds to evaluate (i) adhesion of K562 cells and (ii) transfection of cells with Lipofectamine 2000 complexes of plasmid DNA (not siRNA) [29]. K562 cells seeded on gelatin-functionalized PCL surfaces were able to adhere and proliferate while cells seeded on PCL did not proliferate.…”