Factorial design, preparation and characterization of new beads formed from alginate, polyphosphate and glycerol gelling solution for microorganism microencapsulation

Santos, Glauber Eduardo de Oliveira; Locatelli, Gabriel Olivo; Coêlho, D. A.; Botelho, P. S.; Amorim, M. S. de; Vasconcelos, T. C. L. de; Bueno, Luciano A.

doi:10.1007/s10971-015-3705-5

Cited by 15 publications

(12 citation statements)

References 19 publications

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“…The encapsulated spores presented better viability in the wet microparticles at both stored temperatures. The dry microparticles presented better viability of the stored spores at 5 • C. As described by Locatelli et al (2018) and dos Santos et al (2015), our viability results are in line, since it was possible to maintain the viability of encapsulated T. harzianum spores.…”

Section: Discussionsupporting

confidence: 88%

“…Microparticles composed of sodium alginate, sodium polyphosphate and glycerol, in different proportions were used to encapsulated containing T. harzianum. They were encapsulated by ionic gelation methodology as the same used in this study (dos Santos et al, 2015). The FTIR characterization showed a peak at around 1019 cm −1 is characteristic of C-O-C bonds.…”

Section: Discussionmentioning

confidence: 99%

“…The microparticles were prepared using the ionic gelation method, which involved dropping a suspension of sodium alginate (ALG -Sigma-Aldrich) containing the organism into a solution of calcium chloride (CaCl 2 -Synth) (dos Santos et al, 2015). A 2% ALG solution was prepared, followed by addition of the fungus powder (2%, w:v -Ecotrich-Ballagro) and agitated for 1 h to obtain a homogeneous mixture.…”

Section: Preparation Of Calcium Alginate Microparticlesmentioning

confidence: 99%

“…For counting the colonies in both microparticles (dried and wet), it was necessary to dilute the microparticles so that the spores could be counted. The dilution was done in 2% sodium citrate (Synth) solution for 20 min, under magnetic stirring (dos Santos et al, 2015). For CFU g −1 analysis, the microparticles were serially diluted in saline solution [0.85% w/v NaCl (Synth)] containing Tween 80 (0.1%).…”

Section: Quantification Of Sporesmentioning

confidence: 99%

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Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Maruyama

Bilesky-José

Lima

et al. 2020

Front. Bioeng. Biotechnol.

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Trichoderma harzianum is a biological control agent used against phytopathogens and biostimulation in agriculture. However, its efficacy can be affected by biotic and abiotic factors, and microencapsulation has been used to maximize the efficacy. The objective was to develop polymeric microparticles to encapsulate T. harzianum, to perform physicochemical characterization to evaluate its stability, to evaluate effects on the soil microbiota, antifungal activity in vitro and enzymatic activity. Size distribution of wet and dry microparticles was 2000 and 800 µm, respectively. Scanning electron microscopy showed spherical morphology and encapsulation of T. harzianum. Photostability assays showed that encapsulation protected the fungus against ultraviolet radiation. The evaluation of the microbiota showed that the proportion of denitrifying bacteria increased when compared to the control. The T. harzianum encapsulation showed an improvement in the chitinolytic and cellulosic activity. In vitro tests showed that encapsulated fungus were able to provide a greater control of S. sclerotiorum.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Calcium Alginate Microparticlesmentioning

confidence: 99%

Section: Quantification Of Sporesmentioning

confidence: 99%

See 2 more Smart Citations

Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Maruyama

Bilesky-José

Lima

et al. 2020

Front. Bioeng. Biotechnol.

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show abstract

“…Thus, the beads were formed and cured for 30 min in the solution. After being taken out of the solution, the beads were washed three times with ultrapure water, and were allowed to dry for 12 hours at 40 °C in a circulating air oven [35]. The compositions of different formula beads are shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Controlled Release of Biological Control Agents for Preventing Aflatoxin Contamination from Starch–Alginate Beads

Feng

Dou

et al. 2019

Molecules

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For the wise use of fungal biocontrol and metalaxyl fungicide, starch–alginate-based formulations have been developed by encapsulating metalaxyl and non-toxigenic Aspergillus flavus spores simultaneously in the form of microspheres using calcium chloride as a cross-linking agent. The formulations were characterized by Fourier transform infrared spectroscopy (FTIR), a scanning electron micrograph (SEM), and thermogravimetry (TGA). Formulation characteristics, including the bead size, entrapment efficiency, swelling ratio of the beads, and rheological properties, were analyzed. The release behavior of beads with different formulations was evaluated. The addition of kaolin and rice husk powder in starch–alginate beads retarded the release profile of spores and metalaxyl. The release of the active ingredient from starch–alginate–kaolin beads and starch–alginate–rice husk powder beads occurred in both a controlled and sustained manner. Additionally, the release rate decreased with the increase of kaolin or rice husk powder content. The beads added with kaolin were slower than the release of rice husk powder. In comparison, spores released slower and lasted longer than metalaxyl. The starch–alginate–kaolin formulations could be used as controlled release material in the field of biocontrol and reduce the harm of fungicides to the environment.

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Viability of microencapsulated species of Trichoderma as a strategy to optimize use in biological control

Pinotti,

de Oliveira Sousa,

Fonseca

et al. 2024

Braz J Microbiol

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Factorial design, preparation and characterization of new beads formed from alginate, polyphosphate and glycerol gelling solution for microorganism microencapsulation

Cited by 15 publications

References 19 publications

Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Controlled Release of Biological Control Agents for Preventing Aflatoxin Contamination from Starch–Alginate Beads

Viability of microencapsulated species of Trichoderma as a strategy to optimize use in biological control

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