Vaccination is one way to prevent disease transmission in livestock. This study was conducted to know the willingness of Balinese cattle farmers to adopt vaccinations from the animal health service unit. The research location was conducted in the Bone district in 2021. The number of samples was 115 farmers who were taken randomly. The primary data came in the form of characteristics of beef cattle farmers and their farms, such as gender, age, education, experience in raising livestock, family size, herd size, contact with veterinarians, and contact with extension workers. The dependent variable was the willingness to adopt vaccination which was measured by dichotomous variables: Yes, if farmers were willing to have their cattle vaccinated, and no, if farmers were not willing to have their cattle vaccinated. The data were processed using SPSS version 23 using the Logit regression model. The results showed that 47.2% of the independent variables affected the dependent variable. The remaining 52.8% was influenced by other factors outside the model. Simultaneously, the dependent variable was affected by the independent variables (P<0.05). Partially, farmers’ age, education, and family size had a significant effect (P<0.05) on whether farmers adopted vaccination from the animal husbandry service unit.