Understanding how groundwater contribution changes with increasing drainage area is an important research challenge for catchment hydrology (Condon et al., 2020;Fan, 2019). Although hydrological and biogeochemical studies of headwater catchments have often assumed that the underlying bedrock was impermeable, many studies have revealed that non-negligible amounts of water and solutes infiltrate into bedrock and leave headwater catchments not detected at the weir (e.g., Aishlin & McNamara, 2011;Frisbee et al., 2016;Iwasaki et al., 2016;Schaller & Fan, 2009). On the other hand, hydrogeology studies have revealed the existence of regional groundwater flows with larger spatial scales than local groundwater flows (e.g., Ono et al., 2019;Tóth, 1962). In the current study, local groundwater contributing to headwater streams and regional groundwater not contributing to them were referred to as shallow and deep groundwater, respectively. Small headwater catchments are perched on top of regional groundwater systems, and deep groundwater usually discharges to lower basins (Fan, 2019;Smerdon et al., 2012). Thus, understanding the scaling relationships of groundwater contributions is essential when applying small headwater catchment study findings to larger catchments.The role of groundwater flow in scaling relationships has been examined by observing specific discharge (discharge per unit drainage area) and stream water chemistry during baseflow periods at multiple points with different drainage areas in a watershed. Shaman et al. (2004), investigating low-flow specific discharge in the Neversink River watershed, USA, which is underlain by sedimentary bedrock, suggested that deep groundwater contributions increase with increasing drainage area in subcatchments smaller than a representative elementary area (REA) of 8-21 km 2 but become constant in subcatchments larger than the REA. Asano et al. (2020) found an increase in baseflow-specific discharge with drainage area in a 93.58-km 2 sedimentary watershed of the Arakawa River, Japan. In addition to baseflow-specific discharge, stream and spring baseflow chemistry have been commonly used to understand groundwater dynamics across spatial scales by separating stream water into shallow and deep groundwater. Two-source separation has shown an increasing ratio of deep groundwater with drainage area in a 5-km 2 sedimentary Inokawa catchment,