Factors Affecting Ex vitro Rooting in Micropropagated Shoots from Nodal Explants of Terminalia arjuna

Abstract

The present work was done with the aim to study the effect of rooting mixture and incubation temperature on Ex vitro rooting of Terminalia arjuna, an important multipurpose tree. The nodal explant collected from Ummaid garden Jodhpur was subjected for In vitro shoot proliferation on BAP supplemented modified MS medium. These shoots were In vitro multiplied on BAP (half concentration of BAP used in In vitro shoot proliferation) with low concentration of NAA supplemented medium. The individual shoots from In vitro multiplied shoots were pulse treated with IBA for 10 min. and transferred in different rooting mixture and incubation temperature for Ex vitro rooting. Analysis of data revealed that maximum 62.22% rooting was observed when the plantlet pulse treated with 984.25 µM IBA for 10 min were transferred on bottle containing vermiculite as rooting mixture and incubated at the temperature of 26°C.The optimization of Ex vitro rooting mixture and temperature conditions will be helpful in propagation of this important species rapidly in large scale.

Problems Encountered during In vitro Culture Establishment in Terminalia arjuna

“…Therefore, the main aim of this study was to offer a solution for the problems occurred during in vitro propagation of T. arjuna due to the recalcitrant nature of this species limiting thus culture establishment and mass production. This study is the continuation of previous research works done in 2015 [5], 2018 [6], 2020 [7] including a complete micropropagation and factor influencing ex vitro rooting in Arjun tree.…”