Factors Affecting Lipase Production in Rhizopus nigricans

Chander, Harish; Batish, V. K.; Ghodekar, D. R.; Srinivasan, R. A.

doi:10.3168/jds.s0022-0302(81)82553-2

Cited by 14 publications

(9 citation statements)

References 12 publications

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“…This means that the terrestric acid probably caused the early cells aging. These results are in agreement with Chander (1981) who reported that the high concentration of the carbon source causes the production of mycotoxins in molds. In addition, Rouvier (2002) and Meisner (1974) showed that terrestric acid is produced in the Krebs cycle, a process that occurs in mitochondria, which enhance its involvement in the respiratory cell metabolism.…”

Section: Metabolic Responsesupporting

confidence: 93%

Effect of Sucrose on the Physiology and Terrestric Acid Production of Penicillium Aurantiogriseum

Bouhoudan¹,

Chidi²,

Khaddor³

2020

AgricultForest

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Penicillium aurantiogriseum (P. aurantiogriseum) is a post-harvest pathogen that causes significant losses in agricultural production during storage. It plays an important role in food and feed spoilage, and it contaminates agricultural products with mycotoxins that are potentially harmful to human and animal health. P. aurantiogriseum is one of the most toxic species in the genus Penicillium, and it is often isolated from foods, vegetables, fruits and permafrost sediments from the Arctic and Antarctic. It has also been isolated from the marine environment. Thus, it is resistant to several types of stress related to nutrients and growing conditions. This study aimed to determine the effect of sucrose on the physiology of P. aurantiogriseum in order to control its growth and toxigenesis. Mycotoxin production was determined by TLC technique. Our results show a close relationship between the physiological state of P. aurantiogriseum and the secretion of mycotoxins under carbon stress conditions. The physiological state of the pathogen reveals a correlation between increased sucrose concentration and the intensity of aging signs. Aging signs begin to disappear at a sucrose concentration of 400 g/l, which allows the normal characteristics of P. aurantiogriseum to reappear. It is suggested that this transformation is meant to avoid the action of sucrose. Terrestric acid production was recorded at the time of appearance of aging signs. Terrestric acid is always maintained, even after returning to a normal physiological state, but its production was diminished. The growth of P. aurantiogriseum can be controlled by modifying the sucrose concentration in growth medium. This allowed us to determine the critical concentration at which the pathogen suffered and thus reached the phase of decline earlier while mycotoxin production was minimal.

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Section: Metabolic Responsesupporting

confidence: 93%

Effect of Sucrose on the Physiology and Terrestric Acid Production of Penicillium Aurantiogriseum

Bouhoudan¹,

Chidi²,

Khaddor³

2020

AgricultForest

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“…The temperature and period of incubation, and effect of pH were studied as described by Chander et al (1981).…”

Section: Methodsmentioning

confidence: 99%

Purification and characterization of the extracellular and cell-bound lipases from a Penicillium cyclopium variety

Druet

Abbadi

Comeau

1992

Appl Microbiol Biotechnol

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A new strain of Penicilfium cyclopium was f o u n d on m o u l d y G r e n o b l e walnuts. F r o m the culture filtrate, two new extracellular lipases (A-and B-lipase) were isolated and characterized. The mycelium contained one o f the two forms of lipase (B-lipase). The specificity of the cell-bound lipase linked to the mycelium was then studied. The enzyme was specific to shortchain h o m o g e n e o u s triacylglycerols; it preferred to attack the o-position rather t h a n the p -p o s i t i o n , and showed stereospecificity on the sn-1 position.

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“…This created a situation of carbon limitation and releases the culture to the carbon source regulation (Aharonowitz and Demain, 1978). It has been shown in several works (Chander et al, 1981;Flipphi et al, 2009;Zain et al, 2011) that the production of antibiotics and other secondary metabolites is negatively affected when the carbon source is rapidly used for growth; however, when the carbon source is slowly introduced into the culture, the secondary metabolite is efficiently produced.…”

Section: Effect Of Sucrose Concentration On Mycotoxin Productionmentioning

confidence: 99%

“…These results have a low value compared to that obtained in agitated medium, suggesting that in the non-agitated medium, the growth and production of lipase was limited by the lack of oxygen used during respiration. The agitation created a kind of aeration in the colony to release CO2 and use the maximum of O 2 in the medium (Börjesson et al, 1990;Chander et al, 1981).…”

Section: Effect Of Sucrose Concentration On Mycotoxin Productionmentioning

confidence: 99%

THE EFFECT OF CARBON SOURCE CONCENTRATION ON TOXIGENESIS AND LIPASE ACTIVITY OF Penicillium aurantiogriseum

Bouhoudan¹,

Chidi²,

Tantaoui-Elarak³

et al. 2018

AgricultForest

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Penicillium aurantiogriseum (P. aurantiogriseum) is a species frequently contaminates olives and other foodstuffs including cereals and their derivatives. Because of its secondary metabolites, P. aurantiogriseum is used in the food and pharmaceutical industries. The aim of this work is to study the influence of carbon source concentration on the secondary metabolites production of P. aurantiogriseum in order to fight against this species and also to improve the production of its lipases. Lipase activity was measured by the titrimetric method with olive oil as a substrate. Mycotoxins production was determined by TLC technique. Our study revealed that P. aurantiogriseum secretes three mycotoxins (terrestric acid, penicillic acid, and aurantiamine) as well as two types of lipase, namely lipases I and II. The production of penicillic acid was detected in CYA, BM, and YES media, while aurantiamine was produced only in BM medium and terrestric acid in YES. Terrestric acid production increased proportionally to the sucrose concentration in YES. However, the penicillic acid production is inversely proportional to the concentration of sucrose in YES. On the other hand, a maximum of lipase activity was obtained in the CYA* medium under agitation condition.

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Factors Affecting Lipase Production in Rhizopus nigricans

Cited by 14 publications

References 12 publications

Effect of Sucrose on the Physiology and Terrestric Acid Production of Penicillium Aurantiogriseum

Effect of Sucrose on the Physiology and Terrestric Acid Production of Penicillium Aurantiogriseum

Purification and characterization of the extracellular and cell-bound lipases from a Penicillium cyclopium variety

THE EFFECT OF CARBON SOURCE CONCENTRATION ON TOXIGENESIS AND LIPASE ACTIVITY OF Penicillium aurantiogriseum

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