Factors Affecting Molecular Weight and Branching Analysis of Metallocene Catalyzed Polyolefins Using On-Line GPC with Light Scattering, and Viscometry Detection

Havard, Trevor; Wallace, Peter

doi:10.1021/bk-1999-0731.ch017

Cited by 4 publications

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“…The conformation plot for a linear polymer in a thermodynamically good solvent will have a slope of approximately 0.58. This has been shown in the literature for numerous polymers including HyA. ,− We characterized several of the linear HyA molecules used to synthesize the MVCs and generated an average conformation plot with a linear regression line shown in Figure . This data for the linear HyA was previously published in Altiok et al As can be seen from this plot, the slope for the linear hyaluronic acid is 0.59, which is very close to the expected slope of around 0.58.…”

Section: Resultsmentioning

confidence: 60%

“…However, for either copolymers or our MVCs, the chemistry of the branch is different from the polymer backbone, so we used linear HyA to make the branching calculations, since this was the backbone chemistry of the MVCs. The literature on branching analysis of heterogeneous copolymers demonstrates a similar method of using a linear counterpart that is chemically identical to the polymer backbone even though the branches of the copolymer have different chemistry. , While this approach makes the assumption that the linear HyA behaves the same as the sFlt-HyA MVC, this turns out to be sufficient to obtain branching analysis data that is in good agreement with the data determined using conjugate analysis. Based on this assumption, we used the k and b values from the linear HyA regression line (Figure ) as the linear counterpart in the equation for branching ratio, Figure .…”

Section: Resultsmentioning

confidence: 96%

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Branching Analysis of Multivalent Conjugates Using Size Exclusion Chromatography–Multiangle Light Scattering

2016

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Multivalent conjugates (MVCs) (conjugation of multiple proteins to a linear polymer chain) are powerful for improving the bioactivity and pharmacokinetics of a bioactive molecule. Since this effect is highly dependent upon the valency of the conjugated proteins, it is imperative to have a technique for analysis of the conjugation ratio. Studies of MVCs have used size exclusion chromatography-multiangle light scattering (SEC-MALS), which allows for the separate and individual analysis of the protein and biopolymer components based on their specific refractive index increment and UV extinction coefficient constants to determine the number of proteins bound per biopolymer molecule. In this work, we have applied traditional branching analysis to the SEC-MALS data, with the primary assumption that the polymer backbone can be used as the linear counterpart. We demonstrated good agreement between the branching values and the valency determined by traditional analysis, demonstrating that branching analysis can be used as an alternative technique to approximate the valency of MVCs. The branching analysis method also provides a more complete picture of the distribution of the measured values, provides important branching information about the molecules, and lowers the cost and complexity of the characterization. However, since MVC molecules are both conjugate molecules and branched molecules, the most powerful approach to their characterization would be to use both traditional multivalent conjugate analysis and branching analysis in conjunction.

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Section: Resultsmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 96%

Branching Analysis of Multivalent Conjugates Using Size Exclusion Chromatography–Multiangle Light Scattering

2016

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“…In order to do so, we use Equation (8). For a molecule eluting at the same elution volume as the linear molecule with degree of polymerization P l , the molecule must have a number of side chains attached, as specified by Equation (8).…”

Section: Resultsmentioning

confidence: 99%

Estimating the Polydispersity of GPC Slices Due to Coeluting Comb-Polymers Synthesized by Grafting Monodisperse Side Chains Onto a Backbone Having Broad Molecular Weight Distribution

Radke¹

2001

Macromol. Theory Simul.

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The molecular weight distribution, polydispersity and the distribution of side chains within a GPC‐slice have been calculated for coeluting comb‐shaped polymers. It is assumed that the polymers were synthesized by grafting monodisperse side chains onto a backbone having a broad molecular weight distribution. Despite the broad polydispersity of the backbone the polydispersity within a GPC‐slice is rather narrow, as is the distribution of side chains. Consequently the effect of polydispersity on properties, which can be obtained by GPC coupled with molar mass sensitive detectors is negligible. However, this result is true only for the specific branching mechanism investigated.

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Molecular Structural Characterization of Polyethylene

deGroot

Gillespie

Cong

et al. 2017

Handbook of Industrial Polyethylene and Technology

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Factors Affecting Molecular Weight and Branching Analysis of Metallocene Catalyzed Polyolefins Using On-Line GPC with Light Scattering, and Viscometry Detection

Cited by 4 publications

References 0 publications

Branching Analysis of Multivalent Conjugates Using Size Exclusion Chromatography–Multiangle Light Scattering

Branching Analysis of Multivalent Conjugates Using Size Exclusion Chromatography–Multiangle Light Scattering

Estimating the Polydispersity of GPC Slices Due to Coeluting Comb-Polymers Synthesized by Grafting Monodisperse Side Chains Onto a Backbone Having Broad Molecular Weight Distribution

Molecular Structural Characterization of Polyethylene

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