Factors Affecting Oxygen Uptake by Yeast Issatchenkia orientalis as Microbial Feed Additive for Ruminants

Lee, J. H.; Lim, Yoo-Beom; Park, K. M.; Lee, S. W.; Baig, S.Y.; Shin, H.T.

doi:10.5713/ajas.2003.1011

Cited by 13 publications

(9 citation statements)

References 9 publications

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“…Effective degradability, ED 0.02 or 0.05 = a + (bc/(k + c)), where k is the rumen outflow rate assumed to be either 2 or 5%/h describing 2 ruminal retention times (50 and 20 h, respectively). capability varies significantly among yeast strains, and it is affected by the VFA (Lee et al, 2003).…”

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confidence: 99%

Effects of Yeast Culture and Galacto-Oligosaccharides on Ruminal Fermentation in Holstein Cows

Mwenya

Santoso

Sar

et al. 2005

Journal of Dairy Science

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Four nonlactating, ruminally cannulated Holstein cows were used in a 4 x 4 Latin square design, balanced for residual effects, to evaluate the effects of supplementing dairy cow diets with yeast culture (Trichosporon sericeum; YC), galacto-oligosaccharides (GOS), or the mixture of YC and GOS on ruminal fermentation, microbial N supply, in situ degradation, and energy and nitrogen metabolism. Treatments were arranged in a 2 x 2 factorial as follows: 1) basal diet, 2) basal diet plus 10 g/d YC, 3) basal diet plus 2% GOS, 4) basal diet plus a mixture of 10 g/d YC and 2% GOS. Nitrogen losses in urine were lower, and retained N was higher, for cows supplemented with a mixture of YC and GOS. Ruminal pH was lower in cows supplemented with GOS alone compared with other treatments. Total VFA concentration was higher in cows fed control and GOS-supplemented diets than in those fed YC containing diets. The molar proportion of propionate was higher, and the molar proportion of acetate was lower, in cows fed control diets. Microbial N supply was higher in cows fed control diets. There were no major positive effects of supplements observed in this study. However, supplementation of a mixture of YC and GOS had a tendency for synergistic effects on N metabolism and in situ degradation of a soluble fraction of oat straw DM and CP of concentrates compared with supplementation of YC or GOS alone.

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confidence: 99%

Effects of Yeast Culture and Galacto-Oligosaccharides on Ruminal Fermentation in Holstein Cows

Mwenya

Santoso

Sar

et al. 2005

Journal of Dairy Science

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“…Issatchenkia orientalis (syn. Candida krusei , Pichia kudriavzevii ) was experimentally used as probiotic in feed to promote animal health (Lee et al ., ; Koh & Suh, ) or as a candidate for bioethanol production (Kitagawa et al ., ). However, the anamorph form of I .…”

Section: Discussionmentioning

confidence: 99%

“…Issatchenkia orientalis (syn. Candida krusei, Pichia kudriavzevii) was experimentally used as probiotic in feed to promote animal health (Lee et al, 2003;Koh & Suh, 2009) or as a candidate for bioethanol production (Kitagawa et al, 2010). However, the anamorph form of I. orientalis, C. krusei, is reported as a notable pathogen especially for immune-compromised patients (Samaranayake & Samaranayake, 1994) and as a causative agent of mycotic mastitis in cattle (Watts, 1988;Elad et al, 1995;Sheena & Sigler, 1995).…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of the original mycoflora in pelleted feed by PCR-SSCP and qPCR

Dorn‐In¹,

Fahn²,

Hölzel³

et al. 2014

FEMS Microbiol Lett

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Ground feeds for pigs were investigated for fungal contamination before and after pelleting (subsamples in total n = 24) by cultural and molecular biological methods. A fungal-specific primer pair ITS1/ITS5.8R was used to amplify fungal DNA; PCR products were processed for the PCR-SSCP method. In the resulting acrylamide gel, more than 85% of DNA bands of ground feeds were preserved after pelleting. Twenty-two DNA bands were sequenced; all represented fungal DNA. The level of fungal DNA in ground feed samples was equivalent to 4.77-5.69 log10 CFU g(-1) , calculated by qPCR using a standard curve of Aspergillus flavus. In pelleted feed, the level of fungal DNA was in average ± 0.07 log10 different from ground feed. Quantified by cultural methods, the fresh ground feeds contained up to 4.51 log10 CFU g(-1) culturable fungi, while there was < 2.83 log10 CFU g(-1) detected in pelleted feeds. This result shows that, while the process of pelleting reduced the amount of living fungi dramatically, it did not affect the total fungal DNA in feed. Thus, the described methodology was able to reconstruct the fungal microbiota in feeds and reflected a considerable fungal contamination of raw materials such as grains.

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“…Also, a paper published by Tamaki et al [27] showed that there is a glutathione-related detoxification system in the yeast I. orientalis where it can degrade S-(2-nitrophenyl) glutathione and S-(2-nitrophenyl) cysteine without suppressing its cell growth. Many researchers demonstrated that this yeast can rapidly degrade a series of volatile fatty acids, including acetic, propionic, butyric, and lactic acid [28][29][30][31].…”

Section: Isolates Used For Biodetoxificationmentioning

confidence: 99%

Novel Isolates for Biological Detoxification of Lignocellulosic Hydrolysate

Hou-rui

Xiang-Xiang

Silva

et al. 2008

Appl Biochem Biotechnol

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In this paper, two new strians, Issatchenkia occidentalis (Lj-3, CCTCC M 2006097) and Issatchenkia orienalis (S-7, CCTCC M 2006098), isolated from different environments on solid media, were used in the detoxification process of the hemicellulosic hydrolysate of sugarcane bagasse. High-pressure liquid chromatography elution curve of UV-absorption compounds represented by acetic acid, furfural, and guaiacol (toxic compounds found in the hemicellulosic hydrolysate) showed that several chromatographic peaks were evidently diminished for the case of detoxified hydrolysate with isolate strains compared to the high peaks resulted for no detoxified hydrolysate. It was clear that these inhibitors were degraded by the two new isolates during their cultivation process. Fermentation results for the biodetoxified hydrolysate showed an increase in xylitol productivity (Q (p)) by 1.97 and 1.95 times (2.03 and 2.01 g l(-1) h(-1)) and in xylitol yield (Y (p)) by 1.72 and 1.65 times (0.93 and 0.89 g xylitol per gram xylose) for hydrolysate treated with S-7 and Lj-3, respectively, in comparison with no detoxified hydrolysate (1.03 g l(-1) h(-1) and 0.54 g xylitol per gram xylose). This present work demonstrated the importance of Issatchenkia yeast in providing an effective biological detoxification approach to remove inhibitors and improve hydrolysate fermentability, leading to a high xylitol productivity and yield.

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Factors Affecting Oxygen Uptake by Yeast Issatchenkia orientalis as Microbial Feed Additive for Ruminants

Cited by 13 publications

References 9 publications

Effects of Yeast Culture and Galacto-Oligosaccharides on Ruminal Fermentation in Holstein Cows

Effects of Yeast Culture and Galacto-Oligosaccharides on Ruminal Fermentation in Holstein Cows

Reconstruction of the original mycoflora in pelleted feed by PCR-SSCP and qPCR

Novel Isolates for Biological Detoxification of Lignocellulosic Hydrolysate

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