Factors Affecting Production and Germination of Oospores of Three Pythium Species

Ayers, W. A.; Lumsden, R. D.

doi:10.1094/phyto-65-1094

Cited by 100 publications

(42 citation statements)

References 8 publications

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“…Also related to lipid activities are several extracellular elicitin-like proteins (Pi000192, Pi000193, Pi002501, Pi012205), which are proposed to encode sterol-and lipid-transport proteins and modifying enzymes (Nespoulous et al, 1999;Osman et al, 2001). Their induction during mating is of interest since sterols are required for sexual development, but can not be synthesized by oomycetes (Ayers and Lumsden, 1975;Kerwin and Duddles, 1989). The ABC transporters encoded by Pi008242 and Pi013922 might be involved in eZux of the mating hormones, as in true fungi (Christensen et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Gene expression during oosporogenesis in heterothallic and homothallic Phytophthora

Prakob

Judelson

2007

Fungal Genetics and Biology

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Section: Discussionmentioning

confidence: 99%

Gene expression during oosporogenesis in heterothallic and homothallic Phytophthora

Prakob

Judelson

2007

Fungal Genetics and Biology

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“…To produce sporangia, Corn Meal Agar plates were inoculated with P. ultimum Puzc, incubated at 22 1C for 3 d, flooded with sterile soil extract (Ayers and Lumsden, 1975), and subsequently incubated at 22 1C for 14-28 d. Sporangia were incorporated into RediEarth as described previously at rates ranging between 0 and 600 sporangia per cm 3 (Roberts et al, 1997). Bacteria and T. virens isolates were applied to cucumber seed in the gelatin formulation.…”

Section: Suppression Of Damping-off Of Cucumber Caused By P Ultimummentioning

confidence: 99%

Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber

et al. 2005

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Lewis, Jack A.; and Chung, Soohee, "Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber" (2005 AbstractThe soilborne pathogens Rhizoctonia solani, Pythium ultimum, and Meloidogyne incognita can cause severe economic losses to field-and greenhouse-grown cucumber. A collection of bacterial isolates and isolates GL3 and GL21 of Trichoderma virens were screened for suppression of diseases caused by these pathogens. T. virens isolates GL3 and GL21 provided the most effective suppression of damping-off caused by R. solani in greenhouse bioassays. Burkholderia ambifaria BC-F, B. cepacia BC-1, and Serratia marcescens N1-14 also provided significant suppression of R. solani relative to the pathogen check in some experiments. T. virens isolates GL3 and GL21 and S. marcescens isolates N1-6, N1-14, and N2-4 provided the most consistent and effective suppression of damping-off of cucumber caused by P. ultimum in growth chamber experiments. No microbial treatment containing individual or combined microbes significantly suppressed populations of M. incognita on cucumber or improved plant vigor in greenhouse bioassays. T. virens GL21 applied as a granular formulation, in combination with B. cepacia BC-1 or B. ambifaria BC-F applied as a seed treatment, significantly improved suppression of damping-off caused by R. solani over individual applications of these microbes in at least one experiment. Treatments combining B. cepacia BC-1, B. ambifaria BC-F, or S. marcescens isolates N1-14 or N2-4 with T. virens GL21 in R. solani biocontrol assays always resulted in plant stands that were similar or greater than treatments containing individual applications of these microbes. B. ambifaria BC-F combined with T. virens GL21 in seed treatments resulted in significantly improved suppression of damping-off caused by P. ultimum in two of three experiments. Populations of T. virens GL3 and GL21 were both substantially reduced after coincubation with B. cepacia BC-1, or S. marcescens isolates N1-14 or N2-4 for 10 to 12 d in cucumber rhizospheres. Populations of T. virens GL21 were slightly reduced after coincubation with B. ambifaria BC-F. Results presented here substantiate other studies reporting enhanced biocontrol performance This article is a U.S. government work, and is not subject to copyright in the United States.with certain combinations of biocontrol agents. These results also indicate that antagonism among combinations of biocontrol agents can vary with the assay system employed. r

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“…Cholesterol (20 mg/l) was added from a 1.5 % solution in 95 % ethanol befor the medium was sterilized at 121°C for 30 min] (Ayers and Lumsden 1975;Abdelzaher et al 1994) at 25°C in the dark for 7 to 14 days. The hyphal debris was removed from the spores by centrifuging the crude spore preparation through a 70 % sucrose pad at 2,500 rpm (Beckman J2-21 M/E).…”

Section: Mycelium and Oospore Preparationmentioning

confidence: 99%

“…Chemical control is difficult because the Pythium spp. forms thickwalled oospores as resting spores for the winter stages of the life cycle; these oospores are the most important initial inoculum (Ayers and Lumsden 1975). Maize stalk rot is still a major threat to maize production because resistant cultivars are still unavailable.…”

Section: Introductionmentioning

confidence: 99%

Rapid and quantitative detection of Pythium inflatum by real-time fluorescence loop-mediated isothermal amplification assay

Cao

et al. 2015

Eur J Plant Pathol

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Pythium inflatum is the causal agent of Pythium maize stalk rot, which is one of the most devastating diseases of maize (Zea mays L.). P. inflatum is currently a major concern in global maize production. To the best of our knowledge, no effective resistance to P. inflatum is known in maize, and no effective measures have been reported for the control of this pathogen once maize plants have been infected. Early and accurate detection of P. inflatum is essential to guide maize planting and to protect maize production. A real-time fluorescence loopmediated isothermal amplification (RealAmp) assay was developed for the rapid quantitative detection of P. inflatum in soil. The detection limit of the RealAmp assay was approximately 0.1 pg/μl plasmid DNA when mixed with extracted soil DNA or 10 3 spores/g of artificially infested soil. No cross-reactions with other related pathogens were observed. Results of the RealAmp assay for quantifying the genomic DNA of P. inflatum were confirmed by testing with artificially and naturally infested samples. The quantification of the soil-borne pathogen DNA of P. inflatum in naturally infested samples was not significantly different compared with classic real-time PCR (P < 0.05). Additionally, the RealAmp assay could be detected via an improved closed-tube visual detection system by adding SYBR Green I fluorescent dye to the inside of the lid prior to amplification. Consequently, the inhibitory effects of the stain on DNA amplification were avoided. Therefore, the assay could be used more conveniently in the field as a simple, rapid, and effective technique and has the potential to become an alternative tool for detecting and monitoring P. inflatum in the field.

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Factors Affecting Production and Germination of Oospores of Three Pythium Species

Cited by 100 publications

References 8 publications

Gene expression during oosporogenesis in heterothallic and homothallic Phytophthora

Gene expression during oosporogenesis in heterothallic and homothallic Phytophthora

Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber

Rapid and quantitative detection of Pythium inflatum by real-time fluorescence loop-mediated isothermal amplification assay

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