Factors affecting the biodegradation of PCP by Pseudomonas mendocina NSYSU

Kao, C. L.; Liu, J.K.; Chen, Y.L.; Chai, Chao; Chen, S.C.

doi:10.1016/j.jhazmat.2005.03.051

Cited by 56 publications

(29 citation statements)

References 17 publications

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“…The toxicity of these compounds tends to increase with relative degree of chlorination (Reineke and Knackmuss 1988). Among chlorinated phenols, pentachlorophenol (PCP) has widely been used as wood and leather preservative, owing to their toxic effect on bacteria, mould, fungi and algae (Kaoa et al 2005). PCP is toxic to all life forms as it inhibits the oxidative phosphorylation (Yang et al 2006) and extensive exposure to PCP could cause cancer, acute pancreatitis, immunodeficiency and neurological disorders (Sai et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Degradation of pentachlorophenol by Kocuria sp. CL2 isolated from secondary sludge of pulp and paper mill

2010

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A pentachlorophenol (PCP) degrading bacterium was isolated and characterized from sludge of pulp and paper mill. This isolate used PCP as its sole source of carbon and energy and was capable of degrading this compound, as indicated by stoichiometric release of chloride and biomass formation. Based on morphology, biochemical tests, and 16S rRNA gene sequence analysis this strain was identified as Kocuria sp. CL2. High Performance Liquid Chromatography (HPLC) analysis revealed that this strain was able to degrade PCP up to a concentration of 600 mg/l. This is first time we are reporting the degradation of PCP by the Kocuria species. This isolate was also able to remove 58.64% of PCP from the sludge within two weeks. This study showed that the removal efficiency of PCP by CL2 was found to be very effective and can be used in degradation of PCP containing pulp paper mill waste in the environment.

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Section: Introductionmentioning

confidence: 99%

Degradation of pentachlorophenol by Kocuria sp. CL2 isolated from secondary sludge of pulp and paper mill

2010

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“…Similar findings with this results had been reported for P. putida epII (Karpouzas and Walker, 2000), where the degradation of ethroprophos was inhibited by succinate or glucose. In other studies, the addition of sodium acetate or glucose did not affect the degradation of pentachlorophenol by P.mendocina NSYSU (Kao et al, 2005), neither did supplementation with glucose improved degradation of phenanthrene (Zhao et al, 2009). Thus, inclusion of other carbon sources may or may not affect the biodegradation of the insecticides by bacteria.…”

Section: Discussionmentioning

confidence: 81%

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

Saafan¹,

Azmy²,

Amin³

et al. 2016

Afr. J. Biotechnol.

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Pseudomonas aeruginosa and Pseudomonas mendocina degrading malathion were studied. Morphological, biochemical and 16S rRNA genes for bacterial identification were selected. Biodegradation of some organophosphorus compounds with the 2 bacterial isolates was determined by high performance liquid chromatography (HPLC). P. aeruginosa strain completely removed diazinon, malathion and fenitrothion, but not chlorpyrifos within 14 days. P. mendocina strain was not able to degrade malathion, diazinon and chlorpyrifos completely and no significant degradation for chlorpyrifos. The bacterial growth curve showed a steady increase in the two bacterial isolates masses during malathion degradation. The highest growth rates were with yeast extract, glucose and citrate for the 2 isolates, but not with phenol. Shaked high inoculum density with incubation at 30°C of malathion bacterial cultures were found to be the optimum conditions for malathion degradation. Bacterial culture extracts subjected to liquid chromatography/mass spectrometry (LC/MS) analysis revealed that the separated products were malathion monocarboxylic acid and malathion dicarboxylic acid. Molecular characterization of carboxylesterase enzyme revealed that carboxylesterase amino acid sequences of the 2 isolates showed high identity to other carboxylesterase enzymes of P. aeruginosa and P. mendocina, respectively. Phylogenetic analysis showed that P. aeruginosa was localized in a separate branch from other carboxylesterase producing Pseudomonas sp. So, it is suggested that this enzyme is a novel esterase enzyme. Use of pesticide-degrading microbial systems for removal of organophosphorus compounds from the contaminated sites requires an understanding of ecological requirements of degrading strains. The results provided an important insight into determining the bioremediation potential of both strains. But the mentioned bacteria cannot be the aim of bioremediation due to risk of public health hazard, hence these bacteria cannot be used in bioremediation but their purified enzymes could.

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“…During this time, they have become serious environmental contaminants. PCP-degrading bacteria are present in soils worldwide (Saber and Crawford 1985;Tiirola et al 2002a;Kao et al 2005;Yang et al 2006;Mahmood et al 2005). Although monochlorophenols and dichlorophenols are produced naturally by some fungi and insects (Gribble 1996), natural sources of PCP are not known; therefore, the degradation pathway(s) employed by bacteria to degrade PCP likely evolved during the approximately 60 years since the human introduction of PCP into the environment (Copley 2000).…”

Section: Pentachlorophenol As a Xenobiotic Environmental Contaminantmentioning

confidence: 99%

“…These include Sphingobium (Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium); Novosphingobium (Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium); and Pseudomonas (Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas). The identification of the latter organism as Pseudomonas mendocina (Kao et al 2005) was originally reported in a thesis (Chai 2002) that is not readily accessible and thus needs confirmation. An actinobacterium (Rhodococcus chlorophenolicus PCP-I = Mycobacterium chlorophenolicum PCP-I) (Briglia et al 1994) isolated in Finland for its ability to degrade polychlorinated phenols (Haggblom et al 1988;Apajalahti and Salkinoja-Salonen 1987a, b) has been characterized to a lesser degree than S. chlorophenolica with regards to the mechanism it uses to degrade PCP and other polychlorinated phenols.…”

Section: Pentachlorophenol Degraders and The Pcp Biodegradation Pathwaymentioning

confidence: 99%

The recent evolution of pentachlorophenol (PCP)-4-monooxygenase (PcpB) and associated pathways for bacterial degradation of PCP

2006

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Man-made polychlorinated phenols such as pentachlorophenol (PCP) have been used extensively since the 1920s as preservatives to prevent fungal attack on wood. During this time, they have become serious environmental contaminants. Despite the recent introduction of PCP in the environment on an evolutionary time scale, PCP-degrading bacteria are present in soils worldwide. The initial enzyme in the PCP catabolic pathway of numerous sphingomonads, PCP-4-monooxygenase (PcpB), catalyzes the para-hydroxylation of PCP to tetrachlorohydroquinone and is encoded by the pcpB gene. This review examines the literature concerning pcpB and supports the suggestion that pcpB/PcpB should be considered a model system for the study of recent evolution of catabolic pathways among bacteria that degrade xenobiotic molecules introduced into the environment during the recent past.

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Factors affecting the biodegradation of PCP by Pseudomonas mendocina NSYSU

Cited by 56 publications

References 17 publications

Degradation of pentachlorophenol by Kocuria sp. CL2 isolated from secondary sludge of pulp and paper mill

Degradation of pentachlorophenol by Kocuria sp. CL2 isolated from secondary sludge of pulp and paper mill

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

The recent evolution of pentachlorophenol (PCP)-4-monooxygenase (PcpB) and associated pathways for bacterial degradation of PCP

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