Factors affecting the development of Phytophthora alni ssp. alni infections in Alnus glutinosa L.

Štochlová, P.; Novotná, Kateřina; Černý, K.

doi:10.17221/26/2011-jfs

Cited by 6 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of infection of alder with P. alni complex depend on several factors: type of inoculated organ [40,41], method of inoculation [40], environment condition [42], derivation of isolate-host genotype, geographic provenience [37], and, as was shown in our study, from what source the isolates were obtained-diseased trunks or soil from under these trees.…”

Section: Discussionmentioning

confidence: 52%

Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder

Trzewik

Maciorowski

Orlikowska

2021

Forests

View full text Add to dashboard Cite

Phytophthora alni complex (P. × alni, P. × multiformis, P. uniformis) are pathogens attacking alder seedlings and trees, causing significant losses in nurseries and natural tree stands. Decay of alder trees has been observed in Poland for over a dozen years. Overall, 25 Polish isolates of P. × alni obtained from symptomatic alder trunks, rhizosphere soil surrounding infected trees, and nearby natural streams were compared with isolates from symptomatic trunks obtained in France, Belgium and Hungary. Morphologic characterization of mycelium, vegetative and generative organs, temperature effect on mycelium growth, and their pathogenicity were studied. The mycelium growth rate of isolates from symptomatic plants was fastest on Carrot Agar (CA) medium, and from soil and water on Vegetable Agar (V8A) medium. The sizes of zoosporangia varied depending on their origin. The isolates that originated from the soil had the largest zoosporangia. The diameter of the oogonia and antheridia did not differ regardless of their origin. The results of pathogenicity tests of P. × alni isolates obtained from different sources showed that the soil isolates were the most aggressive in each test, followed by the isolates from the trunks and water. A simple test of leaf colonization can give an idea of the aggressiveness of the isolate towards the shoots and roots. No morphological or physiological markers of aggressiveness have been found.

show abstract

Section: Discussionmentioning

confidence: 52%

Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder

Trzewik

Maciorowski

Orlikowska

2021

Forests

View full text Add to dashboard Cite

show abstract

“…Re‐isolations of the pathogen from necroses were consistently successful in a series of inoculations in a pretest, which was partially published in Štochlová et al. (). As carrying out this number of re‐isolations in the main test would be highly time‐consuming, the success of infections was checked visually by noting the presence or absence of necroses in comparison with the controls.…”

Section: Methodsmentioning

confidence: 91%

“…The flasks were then placed in randomized positions in an incubator at 20°C and incubated for 1 week in the dark (Štochlová et al. ). After the incubation period, the segments were quick‐frozen to stop pathogen development (Černý et al.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Variation in Alnus glutinosa susceptibility to Phytophthora ×alni infection and its geographic pattern in the Czech Republic

2015

Self Cite

View full text Add to dashboard Cite

Summary Variation in natural susceptibility of the black alder population to Phytophthora ×alni (PA), the oomycete pathogen causing a devastating disease of alder, and its possible relationship to geographic origin, was studied in vitro using branch inoculation tests. Ninety black alder genotypes from different regions of the Czech Republic and two isolates of PA were employed. Host susceptibility varied significantly. After 1 week of infection, the lesion surface areas ranged from 254 to 2051 mm2 and from 19 to 970 mm2 for the two isolates, respectively. The differences were also dependent on the geographical origin and altitude of the sites from which particular host genotypes were taken. These findings have important implications for restoration plantings and for PA resistance breeding programmes, as there is potential to make selections from natural populations.

show abstract

“…Wound inoculation, either on detached or in situ stems, has been used to test seasonal changes in susceptibility of black alder to P. × alni (Stochlová et al ., ) and of red oaks to P. cinnamomi (Robin et al ., ). In agreement with these studies, the present investigation showed that the highest susceptibility was in summer.…”

Section: Discussionmentioning

confidence: 99%

Assessment of inoculation methods for screening black alder resistance to Phytophthora ×alni

et al. 2015

View full text Add to dashboard Cite

Identification of resistance to Phytophthora 9alni could provide the basis for a management strategy against alder decline in riparian ecosystems in Europe. This study aimed to test methods to evaluate the resistance of riparian alders to the disease, and to screen alder genotypes for resistance. Phytophthora 9alni isolates were compared for their aggressiveness (lesion length on stem) and sporulation capacity (sporangia). While no difference in lesion lengths was found between isolates, sporangia production was dependent on isolate, highlighting the need for careful selection of isolates used for zoospore inoculation methods. Inoculation tests carried out at different periods of the year revealed a seasonal change in susceptibility to the disease, with the period from June to September being the most efficient for inoculation tests. Stem-wounded inoculations, carried out on excised shoots, were unreliable for evaluating the level of resistance of alder genotypes to P. 9alni infection, with divergent results between two successive years or between two inoculation periods during the same year. In contrast, a method that mimics the natural conditions of infection, based on flooding of rooted cuttings in artificially infected river water, was found promising. Another method, based on the inoculation of foliated terminal shoots with zoospore suspensions, was found to be repeatable and could be used for high-throughput analyses. Altogether, the results show a continuous resistance response from highly susceptible to moderately resistant genotypes. This suggests that breeding might be a useful strategy to manage alder decline caused by P. 9alni.

show abstract

Factors affecting the development of Phytophthora alni ssp. alni infections in Alnus glutinosa L.

Cited by 6 publications

References 25 publications

Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder

Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder

Variation in Alnus glutinosa susceptibility to Phytophthora ×alni infection and its geographic pattern in the Czech Republic

Assessment of inoculation methods for screening black alder resistance to Phytophthora ×alni

Contact Info

Product

Resources

About