Factors affecting the in vitro production of bovine embryos in a commercial program

Nogueira, Bárbara Gomes Rodrigues; Souza, Lilian; Puelker, R. Z.; Giometti, Inês Cristina; Firetti, Sheila Merlo Garcia; Dias, Tatiane Szücs dos Santos Barros; Castilho, Caliê

doi:10.33448/rsd-v10i2.12264

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…In Black Japanese cows in the hot (28.3 ± 0.3 °C, 66.5% RH) and cool (17.5 °C, 62.9% RH) seasons, 11.2 ± 0.8 and 23.2 ± 1.9 COCsT were obtained by OPU [35] and in Gyr cows for 14 days in controlled environments at 25 and 38 °C, 11.2 ± 2.8 and 14.3 ± 2.5 COCsT were obtained [36]. In addition to climatological and environmental factors, genotype, aspiration frequency and semen used, among others, affect COCsT in an OPU-IVF program, [37]. In this for each kg of increase in BW of the donors, the COCsT increased with 𝛽 ̂= 0.002±0.001, well fed donors have greater energy reserves that are used in reproductive events [38].…”

Section: Discussionmentioning

confidence: 92%

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

Rosales-Martínez,

Becerril-Pérez,

Rosendo-Ponce

et al. 2024

Preprint

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One of the main factors that influence the fertility of cattle in grazing systems in hot tropical climates is heat stress. The objective of this study was to evaluate the effect of season, breed, hormonal and physiological condition on the quantity and quality of cumulus-oocyte complexes (COCs) and embryos produced in vitro, from Romosinuano (RM) and Tropical Milking (TM) donors. Three ovum pick-up and in vitro fertilization (OPU-IVF) were performed, one per season; hot dry (HD; 10, 10), hot humid (HH; 9, 9) and fresh dry (FD; 7, 10) in RM and LT donors. Serum levels of cortisol, insulin and glucose were measured, in addition to heart rate (HR), respiratory rate (RR) and rectal temperature (RT). Effect of season x genotype interaction (p≤0.05) was observed in all COCs variables and only in cleavage embryos (CLI) (p≤0.05). Body weight (BW) affected all COCs variables (p≤0.01), except unviable (UNV) although affected degenerated embryos (DEG) (p≤0.01) and total blastocysts (BLAST) (p≤0.01). Cow age only affected viable COCs (VIAB) (p ≤ 0.05), code one blastocysts (BC1) and BLAST (p≤0.01). Cortisol affected total COCs (COCsT), VIAB and total matured in vitro (TMIV) (p≤0.01), as well as CLI, BC1 (p≤0.01) and BLAST (p≤0.05). Insulin affected COCsT (p≤0.01), UNV (p≤0.05), denuded oocytes (DE) (p≤0.01), BC1 and code two blastocysts (BC2) (p≤0.01). Glucose affected all COCs variables (p≤0.01), except UNV and all embryo variables except BC2. HR affected COCsT, DE, TMIV (p≤0.01), CLI, BLAST and DEG (p≤0.05). RR affected COCsT, UNV, VIAB, CLI (p≤0.05), BC1, BLAST and DEG (p≤0.01). RT only affected DE, VIAB (p≤0.01) and BLAST (p≤0.05). The seasonal climatic year variation of Veracruz and changes in physiological and hormonal variables have diverse effects on the cumulus-oocyte complexes and embryos produced by RM and TM donors.

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Section: Discussionmentioning

confidence: 92%

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

Rosales-Martínez,

Becerril-Pérez,

Rosendo-Ponce

et al. 2024

Preprint

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“…The recovery of grade C oocytes was higher than grades A, B, D and E oocytes in the present study. Results reported by Looney et al (1994), Manik et al (2003), Chaubal et al (2006), Sakhong et al (2012) Nogueira et al (2021), breed, blood group and frequency of aspirations have a strong influence on embryonic production. Wilson et al (2006) produced more number of grade 2 embryos followed by grade 1 and grade 3 respectively in contrast to the present study.…”

Section: Grading Of Embryosmentioning

confidence: 86%

Ovum Pick Up and in vitro Embryo Production using Sex Sorted Semen in Cows

Hadimani,

Khillare,

Amle

et al. 2024

IJAR

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Background: Ovum pick-up, a technique for extracting immature oocytes from antral follicles, with in vitro embryo production, is a way to produce large number of embryos from live donors. Methods: By transvaginal ultrasound-guided ovum pick-up, oocytes were collected from large (9-19 mm), medium (4-8 mm) and small (2-3 mm) ovarian follicles of donor cows (n=8). The recovered oocytes were classified as A, B, C, D and E grades. Oocytes were subjected toin vitro maturation, fertilization (with sex sorted semen) and culture. Cleavage and blastocyst rates were recorded on day 4 and day 7 of culture respectively. Result: Total of 278 follicles were aspirated. The follicles aspirated per cow per OPU session was 12.67±1.86. The oocyte recovery rate was 45.31±7.35. Mean percentage of grade A, B, C, D and E oocytes was 15.69±3.91, 24.97±3.02, 37.53±3.45, 19.44±3.76 and 2.38±2.38 respectively. In vitro maturation rate (IVM%) was 91.71±2.38% and fertilization rate was 90.18±1.52%. Cleavage and blastocyst rates observed was 55.1±5.90 and 24.81±2.95% respectively. The mean percentages of different grades of embryos observed was, code 1: 21.13±2.78, code 2: 14.41±3.25, code 3: 19.56±4.11 and code 4: 44.9±5.90%. Transvaginal ovum pick up can be performed repeatedly without hormonal stimulation. Sex sorted semen can be used for in vitro production of female embryos.

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“…Sperm straws were thawed for 30 seconds in 37  C water after being frozen. After thawing, a discontinuous Percoll gradient was used (Sigma GE17-0891-01) (45 and 90% (vol/vol) Percoll) (Maziero et al, 2020;Nogueira et al, 2021). Semen was inserted in a 15 mL tube with 5 ml capacitation medium.…”

Section: In Vitro Fertilization Of Oocytesmentioning

confidence: 99%

“…The final stage of in vitro production (IVP) is the in vitro culturing of bovine embryos, which begins with a zygote culture and normally takes 7 to 8 days. At this stage, the embryo undergoes its first cleavage division, the embryonic genome is activated and the morula and blastocyst are created (W renzycki, 2018;Ramos-Deus et al, 2020;Nogueira et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Impact of Ionomycin on the Gene Expression of In vitro Fertilized Bovine Embryos

Amran,

Alhimaidi,

Ammari

et al. 2024

IJAR

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Background: The embryo genomic material used in this study came from an equal contribution of maternal and paternal genes. Gene expression during IVEP embryo development was controlled by several factors: the genetic makeup of the embryo, the epigenetic contributions and the culture materials. The aims of this research were to study the impact of ionomycin treatment on the sperm capacitation time and its impacts on the gene expression of some sensitive genes (Bcl-2, Bax, HSPB1, IGF1 and GDF9) during IVEP. Methods: The semen was placed in a sterile 15ml tube containing 5ml of capacitation medium and then split into three different experimental groups: the first group was the control; the second was the low-treated group, which contained sperm in a capacitation medium with 25 nM of ionomycin; the third group included 50 nM ionomycin. Result: The gene expression of BAX during early cleavage did not show any differences. However, in the late cleavage stage, the control group presented higher gene expression (P less than 0.05) than that in the two treated groups. The Bcl-2 genes of the control group showed higher gene expression (P less than 0.05) in both the early and late cleavage stages compared to the low-treated group. Gene expression of the HSP-1 gene was observed during early embryo development in the treated group (25 nM ionomycin). In conclusion, concentration 25 nM ionomycin showed higher gene expression in the early stages of embryo development also the best-quality embryos and gene expression results were found by studying gene expression. The transition from the maternal and paternal genome to the embryonic genome and gene expression during embryo development is controlled by several factors.

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Factors affecting the in vitro production of bovine embryos in a commercial program

Cited by 9 publications

References 30 publications

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

Ovum Pick Up and in vitro Embryo Production using Sex Sorted Semen in Cows

Impact of Ionomycin on the Gene Expression of In vitro Fertilized Bovine Embryos

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