The biochemical composition of seed collected from six landrace accessions of Arachis hypogaea L. subsp. hypogaea var. hirsuta was investigated. Florida-grown runner-(cv. Florunner) and virginia-type (cv. NC 7) seed were used as comparative controls. Fatty acid methyl esters were prepared from hexane-extracted oil and analyzed by gas-liquid chromatography. Oil stability was determined using oxidative stability instrumenta tion. Tocopherols, free amino acids, and free sugars were analyzed by high-performance liquid chromatog raphy. Total oil content ranged from 36-45% for var. hirsuta seed as compared to 46 and 45% for Florunner and NC 7, respectively. Oleic acid/linoleic acid ratios ranged from 0.76-0.95 for the var. hirsuta peanuts as compared to runner (1.71) and Virginia (2.1) controls. Tocopherol levels for var. hirsuta (295-377 ppm in oil) were similar to NC 7 (300ppm) and lower than Florunner (425 ppm). Oil quality characteristics were reflected in much shorter oil stability index times for var. hirsuta seed (5.9-8.0 hr) compared to Florunner (11.6 hr) and NC 7 (12.9 hr). In general, var. hirsuta peanuts con tained more free sugars (141-178 μπιοΐ/g defatted meal) and free amino acids (18.5-37.2 μπιοΐ/g defatted meal) than Florunner (127 and 20.2 μπιοΐ/g defatted meal free sugars and free amino acids, respectively) or NC 7 (122 and 20.3 μπιοΐ/g defatted meal).Key Words: Tocopherols, [oil content, oleic/linoleic acid ratio, free amino acids, «free sugars, oil stability index.The roasted flavor of peanuts is unique and investiga tion of heritability of the roasted peanut component of this complex flavor has been conducted by Pattee and coworkers (Pattee and Giesbrecht, 1990;Pattee et al, 1993). They found that parental lines influence flavor and that the identification of peanut germplasm with high roast flavor quality will be important to improve roast peanut flavor in future cultivars. ^he use of trade names in this publication does not imply endorsement by the USDA or the North Carolina Agric. Res. Serv. of the products named, nor criticism of similar ones not mentioned.