Factors affecting the success of laparoscopic artificial insemination in sheep

Spanner, E.A.; de Graaf, S.P.; Rickard, J.P.

doi:10.1016/j.anireprosci.2024.107453

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Cited by 5 publications

References 226 publications

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Uterine tone influences fertility of Merino ewes following laparoscopic artificial insemination

Spanner,

de Graaf,

Rickard

2024

Theriogenology

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Uterine tone influences fertility of Merino ewes following laparoscopic artificial insemination

Spanner,

de Graaf,

Rickard

2024

Theriogenology

View full text Add to dashboard Cite

Improving the efficacy of progesterone pessary treatment for synchrony of estrus in the sheep

Walker,

Weaver,

Swinbourne

et al. 2025

Theriogenology

View full text Add to dashboard Cite

A multivariate model for the prediction of pregnancy following laparoscopic artificial insemination of sheep

Spanner,

de Graaf,

Rickard

2024

Sci Rep

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The causes of variation in the success of laparoscopic artificial insemination (AI) in sheep are not well understood. As such, this study incorporated the contributions of multiple male and female factors relevant to the success of AI into a comprehensive prediction model for pregnancy success. Data from Merino ewes ( N = 30 254) including age, uterine tone (1; pale/flaccid-5; turgid/pink), intra-abdominal fat (1; little to no fat present-5; high fat), time of insemination and sire used, were recorded during AI. A subset of semen per sire ( N = 388) was thawed and assessed for volume, subjective motility, sperm concentration, and morphology. Sperm motility (CASA), viability and acrosome integrity (FITC-PNA/PI), membrane fluidity (M540/Yo-Pro), mitochondrial superoxide production (Mitosox Red/Sytox Green), lipid peroxidation (Bodipy C11), level of intracellular reactive oxygen species (H 2 DCFDA) and DNA fragmentation (Acridine Orange) were also assessed 0, 3 and 6 h post-thaw. Logistic binomial regression revealed sperm concentration ( P < 0.001), CASA parameters at 0 h (PCA3; P = 0.03), viable acrosome intact sperm at 6 h ( P = 0.02), abnormal morphology ( P < 0.001), uterine tone ( P < 0.001) and intra-abdominal fat ( P = 0.03) of ewes influenced likelihood of pregnancy. Results generated will help standardise the pre-screening and selection of semen and ewes prior to artificial breeding programs, reducing variation in the success of sheep AI. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-79253-x.

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Factors affecting the success of laparoscopic artificial insemination in sheep

Cited by 5 publications

References 226 publications

Uterine tone influences fertility of Merino ewes following laparoscopic artificial insemination

Uterine tone influences fertility of Merino ewes following laparoscopic artificial insemination

Improving the efficacy of progesterone pessary treatment for synchrony of estrus in the sheep

A multivariate model for the prediction of pregnancy following laparoscopic artificial insemination of sheep

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