Factors Affecting the Variation in Response of Mice to Gonadotrophic Hormones

McLaren, Anne

doi:10.1677/joe.0.0370147

Cited by 12 publications

(6 citation statements)

References 20 publications

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“…These results thus confirm earlier observations that oocyte maturation and ovulation are dependent upon the dose of exogenous gonadotrophin administered (e.g. McLaren, 1966McLaren, , 1967Christenson & Eleftheriou, 1972).…”

Section: Discussionsupporting

confidence: 92%

Response of Mouse Ovaries in Vivo and in Organ Culture to Pregnant Mare's Serum Gonadotrophin and Human Chorionic Gonadotrophin

Neal¹,

Baker²

1974

Reproduction

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A series of experiments was carried out to assess the effects of HCG in vivo and in organ culture on oocyte and follicle maturation following treatment with PMSG in vivo. Quantitative histological procedures were used to assess the proportion of follicles in which the oocytes had resumed meiosis beyond the germinal vesicle (diffuse diplotene) stage in response to different doses of hormones. The injection of 3 to 5 i.u. PMSG followed 48 hr later by treatment with 1 to 2 i.u. HCG induced the maximum preovulatory response in ovaries of Schofield albino mice both in vivo and in organ culture. The proportion of large antral follicles with oocytes at metaphase II, however, was much lower in culture than in the intact animal. By contrast, the number of pre-antral and small Graafian follicles increased to a level far higher than in vivo and thus the overall total number of oocytes that progressed beyond the germinal vesicle stage was higher in organ culture.

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Section: Discussionsupporting

confidence: 92%

Response of Mouse Ovaries in Vivo and in Organ Culture to Pregnant Mare's Serum Gonadotrophin and Human Chorionic Gonadotrophin

Neal¹,

Baker²

1974

Reproduction

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“…Compared with the result of Beaumont & Smith (1975), this increase is surprisingly high, as the females were picked randomly regardless of the stage of oestrous cycle. One possible explanation for this is a difference between the mouse strains used (Lin & Bailey, 1965;McLaren, 1967;Luckett & Mukherjee, 1986). Alternatively, the most responsive stage of the oestrous cycle to the superovulatory treatment has not yet been identified.…”

Section: Discussionmentioning

confidence: 99%

Rate of gonadotrophin-induced abnormalities in mouse ova is related to the site of hormone administration

Lehtonen¹,

Kankondi²

1987

Reproduction

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Adult female mice, regardless of the stage of the oestrous cycle, were superovulated with PMSG and hCG. Ovulated oocytes were recovered 20-22 h after hCG and fertilized ova 72-74 h after hCG. Compared with the controls, the gonadotrophin treatment increased the mating rate of the females, and the incidence of abnormal ova. Regardless of the site of gonadotrophin injections, the numbers of ova were equal, but the proportion of abnormal eggs in mice injected intraperitoneally was significantly higher than in mice injected subcutaneously.

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“…Superovulation rates in this study compared favorably to previous reports [Fowler and Edwards, 1957;Gates, 1971;Ackerman et al, 19831. Many factors may affect responses to gonadotropic hormones [McLaren, 1967;Gates, 19711 and other aspects of fertilization and early embryogenesis [Desjardins, 19791. We have noted that female mice, used within 2 weeks of shipment from the supplier to our lab, produced dramatically reduced number of two-cell and total embryos. Often these mice, and rarely acclimated mice, will produce embryos primarily in the one-cell stage or fragmented, yet with some normal-looking two-cell embryos.…”

Section: Discussionmentioning

confidence: 99%

Culture of mouse preimplantation embryos as a quality control assay for human in vitro fertilization

et al. 1984

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Techniques for culturing preimplantation mouse embryos from the two-cell stage to blastocyst are described, and the importance of this system to quality control assay the media and supplements used in human in vitro fertilization (IVF) procedures is discussed. Embryos from B6CBAF, mice were cultured in a commonly used mouse culture medium, modified Krebs-Ringer-bicarbonate (Krebs'), or in a commonly used human culture medium, Ham's FIO nutrient mixture supplemented with human fetal cord serum (FCS), and results were not significantly different. Using the mouse embryo culture system, tests on 174 preparations of FCS resulted in 24.1 % producing less than 75% morula or blastocyst stages after 72 h in culture, compared to 9.5% of the Krebs' control cultures. Rcsults of the mouse embryo culture system using 98 FCS subsequently used in human IVF were compared to results from the VIP Human In Vitro Program of Eastern Virginia Medical School of Norfolk, Virginia, from June 1982 through June 1983. The data suggest that prescreening of media and supplements using this mouse embryo culture system may indicate sources of factors potentially detrimental to the success of human IVF procedures.

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Factors Affecting the Variation in Response of Mice to Gonadotrophic Hormones

Cited by 12 publications

References 20 publications

Response of Mouse Ovaries in Vivo and in Organ Culture to Pregnant Mare's Serum Gonadotrophin and Human Chorionic Gonadotrophin

Response of Mouse Ovaries in Vivo and in Organ Culture to Pregnant Mare's Serum Gonadotrophin and Human Chorionic Gonadotrophin

Rate of gonadotrophin-induced abnormalities in mouse ova is related to the site of hormone administration

Culture of mouse preimplantation embryos as a quality control assay for human in vitro fertilization

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