Factors affecting trace element uptake in the black mussel Septifer virgatus

Wang, Wen‐Xiong; Dei, Robert C.H

doi:10.3354/meps186161

Cited by 60 publications

(69 citation statements)

References 27 publications

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“…In this study, metal uptake from the dissolved phase by the slipper limpet Crepidula onyx proceeded linearly over time, consistent with many previous studies of aquatic invertebrates (George & Coombs 1977, Bjerregaard et al 1985, Wang & Dei 1999b. Because there was substantial accumulation of metals in the shells, measurements of radioactivity in the soft tissues after 8 h of exposure were used to calculate the metal influx rate into the soft tissues, assuming that metal accumulation by the soft tissues also proceeded linearly over time of exposure.…”

Section: Discussionsupporting

confidence: 56%

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Contribution of dietary bacteria to metal accumulation in the slipper limpet

Qiu¹,

Qian²,

Wang

2001

Aquat. Microb. Ecol.

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Recent studies have shown that dietary ingestion can be an important source for metal accumulation in aquatic animals. Whether bacteria can contribute significantly to the overall metal accumulation in these animals remains little known. In this study, we used the kinetic modeling approach to examine the relative contribution of the aqueous phase, bacteria, and phytoplankton, as sources for Cd, Cr, and Zn accumulation by the slipper limpet Crepidula onyx, a suspension feeder that can capture bacteria efficiently. Metal uptake rate constants measured in C. onyx were 0.200, 1.232, and 1.294 l g -1 d -1 for Cd, Cr, and Zn, respectively. The assimilation efficiency (AE) of metals from ingested phytoplankton (11 to 44% for Cd, and 31 to 41% for Zn) was comparable to AEs of metals from ingested bacteria (21 to 42% for Cd, 35 to 47% for Zn), whereas the AEs of Cr from ingested phytoplankton (10 to 22%) were lower than from ingested bacteria (44 to 59%). The AEs of Cr from different phytoplankton and bacterial diets were related to its distribution in the cytoplasm of cells and its passage time across the animal's guts. The limpets filtered the bacteria at rates (17 l h -1 g -1 ) 1.3 to 1.9× lower than the filtration rate on different phytoplankton diets (22 to 33 l h -1 g -1). The estimated average bioconcentration factors of metals by 2 bacterial strains were 2 to 6 × 10 5 for Cd, 1 to 7 × 10 5 for Cr, and 1.5 to 7 × 10 5 for Zn. The kinetic model predicted that uptake from dietary phase dominated metal accumulation in the slipper limpets (87% for Cd, 72% for Cr, and 83% for Zn). Ingestion of bacteria contributed 23% for Cd, 27% for Cr, and 17% for Zn accumulation in the limpets under typical conditions for the limpets. Our study therefore highlights bacteria as a potentially important source of metal accumulation in this filter-feeding mollusc.

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Section: Discussionsupporting

confidence: 56%

“…Filtration rate. Filtration rate of the limpets was determined by monitoring the decrease of algal or bacterial density over time using established methods (Widdows 1985, Wang & Dei 1999b). The algal cell density was determined using a Coulter Multisizer II (Coulter Co., FL, USA).…”

Section: Methodsmentioning

confidence: 99%

Contribution of dietary bacteria to metal accumulation in the slipper limpet

Qiu¹,

Qian²,

Wang

2001

Aquat. Microb. Ecol.

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“…Organelle Cd in the oysters from stations 4 to 8 was 33 to 44%. The MTLP fraction was Metal 30,2011important in binding with As (33-53%), Ni (11-54%), Cu (1-21%), and Zn (13-43%). The HSP was a notable pool for binding with Cd, Cu, and Zn.…”

Section: Subcellular Distribution Of Metals In Oystersmentioning

confidence: 99%

Copper and zinc contamination in oysters: Subcellular distribution and detoxification

Wang

Yang

Guo

et al. 2011

Environmental Toxicology and Chemistry

129

104

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Metal pollution levels in estuarine and coastal environments have been widely reported, but few documented reports exist of severe contamination in specific environments. Here, we report on a metal-contaminated estuary in Fujian Province, China, in which blue oysters (Crassostrea hongkongensis) and green oysters (Crassostrea angulata) were discovered to be contaminated with Cu and other metals. Extraordinarily high metal concentrations were found in the oysters collected from the estuary. Comparison with historical data suggests that the estuary has recently been contaminated with Cr, Cu, Ni, and Zn. Metal concentrations in blue oysters were as high as 1.4 and 2.4% of whole-body tissue dry wt for Cu and Zn, respectively. Cellular debris was the main subcellular fraction binding the metals, but metal-rich granules were important for Cr, Ni, and Pb. With increasing Cu accumulation, its partitioning into the cytosolic proteins decreased. In contrast, metallothionein-like proteins increased their importance in binding with Zn as tissue concentrations of Zn increased. In the most severely contaminated oysters, only a negligible fraction of their Cu and Zn was bound with the metal-sensitive fraction, which may explain the survival of oysters in such contaminated environments.

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“…However, the intra-specific difference in clearance rate cannot completely account for the difference in metal uptake rate constants among the bivalves (Chong & Wang 2001). Smaller oysters had higher Cd uptake rate than the larger oysters in the July populations, similar to that observed for Cd and zinc (Zn) uptake in mussels, clams, and scallops , Wang & Dei 1999, Pan & Wang 2008b. In these bivalves, the influx rate decreased in a power function with increasing body size, which may be caused by size-specific metabolic rates (Boyden 1974, Ringwood 1989, Newman & Heagler 1991 or changes in gill surface area.…”

Section: Discussionmentioning

confidence: 83%

Cadmium accumulation and loss in the Pacific oyster Crassostrea gigas along the west coast of the USA

Ng¹,

Chuang²,

Stupakoff³

et al. 2010

Mar. Ecol. Prog. Ser.

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Oysters can accumulate cadmium (Cd) in very high concentrations in their tissues and there is now increasing concern for the seafood safety of farmed oysters worldwide. Bioaccumulation and biokinetics of Cd (dietary assimilation, uptake from the dissolved phase, and efflux) were measured in different populations of Pacific oyster Crassostrea gigas collected along the west coast of the USA in 2 different seasons. Triploid juvenile oysters were first transplanted to 5 Washington sites and 1 Oregon site for 3 mo, then transported back to the laboratory for tissue Cd measurements and biokinetic experiments. A second group of seed oysters was collected from California and Washington for additional analysis. Oysters from all sites had Cd concentrations lower than the food standard limit and there was spatial difference in tissue Cd concentrations. A significant fraction of Cd was associated with metallothionein-like proteins, implying that the oysters can detoxify Cd by induction of metallothionein. The difference in Cd dietary assimilation (using diatoms as a food source) and efflux in oysters transplanted to different locations was not significant. The uptake rate constants for Cd from the dissolved phase, however, differed markedly among the transplanted populations, and appeared to be affected by the oysters' clearance rate, depending on the population. This uptake rate was also negatively affected by the size of oysters. Dietary assimilation of Cd was comparable at diatom food concentrations below 2 to 5 mg l -1 , and was lower with ingested sediments than with diatoms. Measurements of Cd kinetics in different populations of Pacific oysters under a variety of environmental conditions provide an insight into the Cd accumulation in this commercially important species.

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Factors affecting trace element uptake in the black mussel Septifer virgatus

Cited by 60 publications

References 27 publications

Contribution of dietary bacteria to metal accumulation in the slipper limpet

Contribution of dietary bacteria to metal accumulation in the slipper limpet

Copper and zinc contamination in oysters: Subcellular distribution and detoxification

Cadmium accumulation and loss in the Pacific oyster Crassostrea gigas along the west coast of the USA

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