Factors affecting transformation efficiency of embryogenic callus of Upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens

Jin, Shuangxia; Zhang, Xianlong; Liang, Shuang; Nie, Yong; Guo, Xiaoping; Huang, Chao

doi:10.1007/s11240-004-5209-9

Cited by 100 publications

(80 citation statements)

References 27 publications

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“…Jin et al, (2005) suggested that co-cultivation at 19 0 C for 48 hours was effective for the efficient transformation which was confirmed earlier by Dillen et al, (1997) and Sunilkumar and Rathore (2001).…”

Section: Introductionsupporting

confidence: 69%

See 1 more Smart Citation

Agrobacterium tumefaciens Mediated Genetic Transformation in Coker-312 (Gossypium hirsutum L.) Using Hypocotyls Explants

Jadhav¹,

Katageri²

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Introductionsupporting

confidence: 69%

“…Co-cultivation duration was found uncertain in cotton ranging from 36 to 72 hours, mainly fascinated by the genotype variability (Sunilkumar and Rathore, 2001;Leelavathi et al, 2004;Jin et al, 2005). In present report, hypocotyls were co-cultivated in MS media (0.1 mg/l 2, 4-D + 0.5 mg/l kinetin) (Fig.…”

Section: Genetic Transformation and Regenerationmentioning

confidence: 58%

Agrobacterium tumefaciens Mediated Genetic Transformation in Coker-312 (Gossypium hirsutum L.) Using Hypocotyls Explants

Jadhav¹,

Katageri²

2017

Int.J.Curr.Microbiol.App.Sci

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“…We first tested the pPNZIP::Cry9C vector in tobacco via Agrobacterium tumefaciens-mediated transformation. The two vectors were then introduced to two Gossypium hirsutum cultivars, YZ1 and Y668 by Agrobacterium tumefaciens-mediated transformation as previously described (Jin et al, 2005;Jin et al, 2006;.…”

Section: Vector Construction and Plant Transformationmentioning

confidence: 99%

Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds

Wang

Zhu

Liang

et al. 2016

Sci. China Life Sci.

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A promoter of the PNZIP (Pharbitis nil leucine zipper) gene (1.459 kb) was cloned from Pharbitis nil and fused to the GUS(-glucuronidase) and Bacillus thuringiensis endotoxin (Cry9C) genes. Several transgenic PNZIP::GUS and PNZIP::Cry9C cotton lines were developed by Agrobacterium-mediated transformation. Strong GUS staining was detected in the green tissues of the transgenic PNZIP::GUS cotton plants. In contrast, GUS staining in the reproductive structures such as petals, anther, and immature seeds of PNZIP::GUS cotton was very faint. Two transgenic PNZIP::Cry9C lines and one transgenic cauliflower mosaic virus (CaMV) 35S::Cry9C line were selected for enzyme-linked immunosorbent assay (ELISA) and insect bioassays. Expression of the Cry9C protein in the 35S::Cry9C line maintained a high level in most tissues ranging from 24.6 to 45.5 μg g 1 fresh weight. In green tissues such as the leaves, boll rinds, and bracts of the PNZIP::Cry9C line, the Cry9C protein accumulated up to 50.2, 39.7, and 48.3 µg g 1 fresh weight respectively. In contrast, seeds of the PNZIP::Cry9C line (PZ1.3) accumulated only 0.26 µg g 1 fresh weight of the Cry9C protein, which was 100 times lower than that recorded for the seeds of the CaMV 35S::Cry9C line. The insect bioassay showed that the transgenic PNZIP::Cry9C cotton plant exhibited strong resistance to both the cotton bollworm and the pink bollworm. The PNZIP promoter could effectively drive Bt toxin expression in green tissues of cotton and lower accumulated levels of the Bt protein in seeds. These features should allay public concerns about the safety of transgenic foods. We propose the future utility of PNZIP as an economical, environmentally friendly promoter in cotton biotechnology. tissue-specific promoter, GUS expression, Bt toxin, transgenic cotton plants Citation:Wang, Q., Zhu, Y., Sun, L., Li, L., Jin, S., and Zhang, X. (2016). Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds. Sci China Life Sci 59, 172 -182.

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“…However, visual markers for selection have also been used as these can increase transformation efficiency by reducing the time and the number of materials used at the screening step [7,8]. Transformants expressing reporter genes have been generated for variety of studies.…”

Section: Introductionmentioning

confidence: 99%

Transformation of Antisense Chalcone Synthase (CHS) Gene into Lotus (Nelumbo Nucifera Gaertn.) by Particle Bombardment

Saetiew¹,

Prissadang²,

Nanglak³

et al. 2017

TOBIOTJ

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Abstract:Chalcone synthase (CHS) is a key enzyme in the flavonoid biosynthesis pathway. CHS genes were cloned from genomic DNA and cDNA from the petals of 'Buntharik' white lotus and 'Sattabangkacha' pink lotus by the PCR technique using a specific primer of the CHS gene designed from the GenBank database. Semi-quantitative RT-PCR analysis revealed that the highest CHS gene expression was found in the early budding stage of the pink lotus and was reduced in later stages. Shoot tips from embryos of Buntharik and Rachinee lotus were used to induce shoot clusters by cultivation on a MS medium supplemented with 40 µM NAA and 0.5 µM TDZ for 8 weeks and a MS medium supplemented with 50 µM BA for 8 weeks. An antisense CHS gene (450 bp) from the cDNA of Buntharik lotus was used to construct a plant transformation vector; pCAMBIA1302CHSA. The vector construct was transformed into Buntharik and Rachinee shoot clusters by particle bombardment. After transformant selection and regeneration, two transformants of Buntharik shoot clusters showed GFP green spots and existence of the GFP gene and hptII gene in the genomic DNA amplified by the PCR technique. In the Rachinee transformants, 3 of 5 showed the GFP green spots and the GFP and hptII genes were identified in amplification by PCR. After CHS gene expression analyses by semi-quantitative RT-PCR, two transformed Rachinee shoot clusters had a reduction in CHS gene expression.

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Factors affecting transformation efficiency of embryogenic callus of Upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens

Cited by 100 publications

References 27 publications

Agrobacterium tumefaciens Mediated Genetic Transformation in Coker-312 (Gossypium hirsutum L.) Using Hypocotyls Explants

Agrobacterium tumefaciens Mediated Genetic Transformation in Coker-312 (Gossypium hirsutum L.) Using Hypocotyls Explants

Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds

Transformation of Antisense Chalcone Synthase (CHS) Gene into Lotus (Nelumbo Nucifera Gaertn.) by Particle Bombardment

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