Factors affecting umbilical cord blood stem cell suitability for transplantation in an in utero collection program

Keersmaekers, Christine L.; Mason, Brian A.; Keersmaekers, Jan; Ponzini, Matthew D.; Mlynarek, Ryan A.

doi:10.1111/trf.12340

Cited by 30 publications

(71 citation statements)

References 16 publications

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“…The present study finding revealed a significant difference in the UCB volume and the TNC content produced by the primigravida and the multigravida; in favor to the primigravida women. Conversely, Keersmaekers et al, [7] found no significant power of the gravidity number on the obtained UCB volume and its cellular content in a study included 7,839 in utero collected UCB units at Michigan. This disagreement between the present study finding and that of Keersmaekers and colleagues could be related to the discrepancy between the subjects criteria.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study by Keersmaekers et al (2014), had shown that the neonatal birth weight that met the TNC requirement was 3,503 ± 463 grams compared to 3,215 ± 453 grams for those that did not met the TNC requirement. [7] To calculate the sample size with precision/absolute error meet of 5% and type 1 error of 5%, the following formula was used: Sample size = [2 × (SD obtained from a previous study) 2 × (Z α/2 + Z β ) 2 ] / mean difference obtained from previous study. [11] Therefore, n = [2 × (463) 2 × (1.96 + 0.84) 2 ] / [3,503 -3,215] = 142.02.…”

Section: Sample Size Calculationmentioning

confidence: 99%

“…Number of stem cells per each umbilical cord blood unit is mirrored by its TNC content. [7] 1.3 Aim of the study This study aimed to identify the maternal and neonatal predictive variables for quality of cord blood as a source of stem cells.…”

Section: Quality Of the Umbilical Cord Blood Unitmentioning

confidence: 99%

“…[2] Acceptance or rejection of UCB units for banking are influenced by the number of stem cells per unit, which is mirrored by the total nucleated cell (TNC) content in each one. [7] Optimizing donor selection could be an abundant target for the nursing staff and obstetricians working in delivery room; they are the main responsible personnel for collecting UCB units.…”

Section: Introductionmentioning

confidence: 99%

“…[10] 1.1 Significance of the study About 54.1% of the collected cord blood units are discarded before processing due to volume deficiency, laboratory issues or shipping obstacles. [7] Research studies seeked for the factors affecting the blood volume and number of TNC obtained from the umbilical cord are inadequate in Egyptian population. Identifying these variables can result in reducing the cost and time spent in collecting, processing, and storing of unsuitable cord blood units.…”

Section: Introductionmentioning

confidence: 99%

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Maternal and neonatal predictive variables for quality of cord blood as a source of stem cells

Marzouk

Abd-Allah²

2018

JNEP

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Objective: This study aimed to identify the maternal and neonatal predictive variables for quality of cord blood as a source of stem cells. Methods: A descriptive design was followed in this study. A purposive sample of 143 pairs; pregnant women assigned for elective cesarean section and their newborns, was enrolled in current study. The subjects were recruited from the Operating Theater of Mansoura University Hospital, Egypt. One hundred forty three umbilical cord blood units were collected in utero from cesarean deliveries. The collected units were assessed for its blood volume and total nucleated cells content. An assessment sheet of 12 items was used as a tool of data collection. Results: The greater blood volume and higher nucleated cells obtained from the primigravida, between 37 to 40 gestation weeks, and those not exposed to cigarette smoke during pregnancy (163.1 ± 24.8 ml and 10.2 ± 2.8 × 10 6 cells/ml, 169.3 ± 22.6 ml and 11.2 ± 1.9 × 10 6 cells/ml, 169.8 ± 21.4 ml and 10.0 ± 2.9 × 10 6 cells/ml respectively). The birth weight ≥ 3,600 g, the first born baby, and the heavier placenta (≥ 550 g) produced significantly greater blood volume and cellular content (161.5 ± 20.6 ml and 9.8 ± 2.4 × 10 6 cells/ml, 164.6 ± 19.4 ml and 9.6 ± 2.7 cells/ml, 164.8 ± 19.6 ml and 10.2 ± 2.6 × 10 6 cells/ml respectively). Conclusions: Primigravida, delivery between 37 to 40 gestation weeks, and no exposure to cigarette smoke during pregnancy were the maternal variables that were associated with larger blood volume and higher cellular content. Even though, first born baby, birth weight ≥ 3,600 g, and placenta weight ≥ 550 g were the neonatal variables.

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Section: Discussionmentioning

confidence: 99%

Section: Sample Size Calculationmentioning

confidence: 99%

Section: Quality Of the Umbilical Cord Blood Unitmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Maternal and neonatal predictive variables for quality of cord blood as a source of stem cells

Marzouk

Abd-Allah²

2018

JNEP

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The effect of maternal and infant factors on cord blood yield

Mousavi

Abroun

Zarrabi

et al. 2016

Pediatr Blood Cancer

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Umbilical cord blood (CB) can be used as an alternative hematopoietic stem cell source for transplantation in hematological malignancy and blood disorders. The success of transplantation is highly related to the levels of total nucleated cell and CD34 cell counts. The evaluation of optimal conditions can decrease the rate of graft rejection due to low cell count and increases the quality of CB units (CBUs) in the blood bank and the success rate of engraftment. To this end, we review the maternal and infant parameters affecting the quality and quantity of CBUs.

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Hematopoietic Stem Cell Collection Techniques

Vaidhyanathan

Easow

2021

Organ and Tissue Transplantation

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Hematopoietic stem cell collection (HPSC) techniques contribute immensely to the outcome of allogeneic and autologous transplants. The major sources of hematopoietic stem cells are the bone marrow, cord blood at birth, or peripheral blood. The technique of collection of HPSC determines the outcome of allogenic and autologous transplants. The techniques adopted should ensure adequate CD34 cell dose with minimal number of collection procedures. Cord blood requires a single, adequate, aseptic collection technique, using cord blood collection kits, at birth, in a well-monitored pregnancy.Allogeneic bone marrow is collected without administration of G-CSF. Allogeneic peripheral blood stem cells (PBSC) require G-CSF mobilization, whereas autologous PBSC collection will require G-CSF alone, G-CSF and chemotherapy, and G-CSF and upfront use of plerixafor to ensure adequate collection of CD34 cell dose to serve dual purpose of proceeding with the transplant and also with a prospect of cryopreserving a suitable cell dose for a possible future tandem transplant. Technological advances and intuitive software incorporated in modern-day cell separators enable PBSC apheresis collection procedures to be done safely with predictable yields. Pre-harvest medical examination is

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Factors affecting umbilical cord blood stem cell suitability for transplantation in an in utero collection program

Cited by 30 publications

References 16 publications

Maternal and neonatal predictive variables for quality of cord blood as a source of stem cells

Maternal and neonatal predictive variables for quality of cord blood as a source of stem cells

The effect of maternal and infant factors on cord blood yield

Hematopoietic Stem Cell Collection Techniques

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