This study aims to analyze the factors associated with the quality of life (QOL) in pediatric dentistry specialists and the like, graduated in a public university between 1972 and 2018. A mixed study (explanatory sequential design) was conducted. Firstly, a cross-sectional survey (n = 62, 51% women) was carried out and complemented with three focus groups (FGs) and four semi-structured interviews (SIs). Descriptive statistics, bivariate analyzes and non-parametric correlation were calculated. A multivariate linear regression was carried out to establish the factors associated with QOL. In FGs, the concept of QOL and the factors that influence it was investigated, by following a qualitative content analysis and later the information was triangulated. The median QOL scores surpass 62 points. The multivariate analysis showed that the factors exerted the greatest influence negatively (decreases the QOL score) were: having an independent or service provision contract (p < 0.05), low social support (p < 0.001), job dissatisfaction (p < 0.01), poor mental health (p < 0.01), self-perceived poor general health (p < 0.01). The information from the FGs and interviews allow to establish three categories of analysis: (1) QOL and health: multiple facets, determinants, and dimensions; (2) encounters and disagreements between the postgraduate curricular training proposal and the labor and social field of the specialist; (3) an itinerant clinical specialization. The QOL of participants is considered good in general terms and is conditioned by subjective factors, the social environment, and the conditions of their professional work.