Factors associated with patency of the uterine cervix in bitches with pyometra

Tamada, Hiromichi; Kawata, Naoko; Kawate, Noritoshi; Inaba, Toshio; Kida, Kenichi; Hatoya, Shingo; Akune, Atsushi; Nakama, Kazuhiro; Kohsaka, Tetsuya; Takahashi, Masahiro; Sawada, Tsutomu

doi:10.1016/j.rvsc.2012.05.012

Cited by 9 publications

(13 citation statements)

References 38 publications

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“…Moreover, the estradiol-induced disruption of collagen bundles has been demonstrated in the cervix of pregnant [22] and estrus [38] ewes. Our results were also in line with the previous report, demonstrating the decreased collagen concentration and increased collagenase activity in relation to cervical relaxation in the bitches with pyometra [30]. The disruption of collagen bundles in this study could be characterized by the increased interfibrillary distance of collagen fibrils likely due to increased water content in the cervical tissue [2, 6].…”

Section: Discussionsupporting

confidence: 93%

“…To date, the mechanism of cervical relaxation in the bitch remains poorly understood in both physiological (estrous cycle) and pathological (pyometra) conditions. Our previous study demonstrated a higher infiltration of neutrophils in the cervix of bitches with open- compared to closed-cervix pyometra [18], and involvement of interleukin-8 expression in the increased cervical patency was suggested [30]. In addition, increased infiltration of mast cells and macrophages was observed in the cervical stroma during estrus [13], and in women, cervical polymorphonuclear cells were increased during parturition [25].…”

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confidence: 99%

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Collagen and Glycosaminoglycan Profiles in the Canine Cervix during Different Stages of the Estrous Cycle and in Open- and Closed-Cervix Pyometra

Linharattanaruksa

Srisuwatanasagul

Ponglowhapan

et al. 2014

The Journal of Veterinary Medical Science

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The extracellular matrix of the cervix that comprises collagen, elastin, proteoglycans and glycosaminoglycans (GAGs) is thought to have an essential role in cervical relaxation. This study investigated the proportion of collagen and smooth muscle as well as the GAGs in cervices obtained from healthy bitches at different stages of the estrous cycle and bitches with open- and closed-cervix pyometra. Cervices were collected after ovariohysterectomy. The proportion of collagen to smooth muscle was determined using Masson’s trichrome staining. Alcian blue staining was used to evaluate the relative distribution of cervical GAGs. The proportion of cervical collagen relative to smooth muscle was higher at estrus compared to anestrus (P≤0.05). It was also higher (P≤0.05) in bitches with open- compared to those with closed-cervix pyometra. Overall, hyaluronan (HA) was the predominant GAG in the canine cervix. In the luminal epithelium, the staining intensity for HA was stronger in estrus than in anestrus (P≤0.05), but not in diestrus (P>0.05). On the contrary, the intensity for the combined keratan sulfate (KS) and heparan sulfate (HS) was stronger in anestrus than in estrus and diestrus (P≤0.05). In bitches with pyometra, the staining intensity of the stroma for KS and HS was weaker in open- compared to closed-cervix pyometra (P≤0.05). Collectively, the different profiles of collagen and GAG suggest that the metabolism of both collagen and GAGs in the canine cervix is associated with hormonal statuses during the estrous cycle and cervical patency of bitches with pathological uterine conditions, such as pyometra.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Collagen and Glycosaminoglycan Profiles in the Canine Cervix during Different Stages of the Estrous Cycle and in Open- and Closed-Cervix Pyometra

Linharattanaruksa

Srisuwatanasagul

Ponglowhapan

et al. 2014

The Journal of Veterinary Medical Science

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“…Since CXCL8 is fundamental for the migration and activation of neutrophils, it is expected to be elevated in diseased animals [ 3 , 6 , 31 ]. Serum CXCL8 levels in dogs with pyometra have seldom been analyzed, but it has been shown that various cell types within the uterus produce CXCL8 in response to diverse stimuli, including pyometra [ 5 , 11 , 16 , 30 ]. The source of CXCL8 measured in sera is unknown and so, a local as well as a systemic secretion has to be taken into consideration.…”

Section: Discussionmentioning

confidence: 99%

Canine pyometra: a model for the analysis of serum CXCL8 in inflammation

Haas

Kaup

Neumann

2016

The Journal of Veterinary Medical Science

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Interleukin-8 (IL-8 or CXCL8) is a highly selective pro-inflammatory chemokine, that is elevated in sera of humans and animals with various inflammatory diseases. CXCL8 is possibly involved in uncontrolled inflammation and the development of a systemic inflammatory response syndrome (SIRS) and sepsis. Nevertheless, its behavior and precise properties in the course of inflammation are not fully understood. Thus, we used naturally occurring canine pyometra as a model of inflammation, in order to examine the behavior of serum CXCL8 in relation to the disease intensity and commonly analyzed inflammatory mediators. Using a commercially available canine ELISA kit, a significant increase of CXCL8 was determined in the serum of 23 dogs with pyometra compared with 35 healthy dogs. Interestingly, serum CXCL8 did not increase in severely diseased patients and behaved contrary to white blood cells (WBC), neutrophils and C-reactive protein (CRP). The measurement of serum CXCL8 may provide valuable information about the extent of ongoing lesions and could be a useful complement for existing laboratory tests.

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“…On the other hand, optical coherence tomography has shown that fiber orientation may vary in some physiological circumstances: in non pregnant women the cervical samples fibers are more regular compared to those in pregnant women [ 18 ]. In pathological circumstances such as in women with pyometra, the fiber orientation pattern may be disrupted because of collagen degradation and connective tissue remodeling [ 19 ], and modification in glycosaminoglycans composition as found during pregnancy or induced by infection may lead to alteration in cervical tensile strength [ 20 ]. We hypothesize that the pathologies that may alter the cervical canal patency may more significantly disrupt the middle zone fiber orientation due to their circumferential direction.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the cervix tissue homogeneity by ultrasound elastography in infertile women for the prediction of embryo transfer ease: a diagnostic accuracy study

Stanziano¹,

Caringella²,

Cantatore³

et al. 2017

Reprod Biol Endocrinol

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BackgroundUltrasound elastography is a non-invasive medical imaging technique able to quantitatively characterize the stiffness of a given tissue. It has been shown to predict the risk for cervical insufficiency and preterm delivery, and to allow differentiation of malignancy from normal tissue. The present study sought to evaluate whether cervical tissue dishomogeneity, as assessed by cervical ultrasound elastography, may predict the embryo transfer (ET) ease in infertile women undergoing IVF/ICSI.MethodsWe evaluated 154 infertile patients with no history of previous ET or intrauterine insemination. Cervical stiffness was evaluated in six regions of interest (ROI), compared two by two to obtain strain ratio (SR) values. Since a SR value of 1 was suggestive of tissue homogeneity, we computed 1-SR/SR-1 values to obtain a measure of the degree of cervical tissue dishomogeneity that we named “dishomogeneity index” (DI). Ultrasound-guided ET was performed by an expert operator blinded to the results of cervical elastography. The prediction ability of elastography on ET ease was evaluated by binary logistic regression, and the predictive accuracy of the independent variables was quantified with area under the curve (AUC) estimates derived from receiver operating characteristic (ROC) curve.ResultsET resulted to be easy in 99 out of 154 patients (64,2%), difficult in 54 patients (35%), and impossible in one. DI values in cervical medial lips region correctly classified 86.9% of patients, according to binary logistic regression, with a sensitivity of 81.4% and a specificity of 89,9%, positive likelihood ratio (LR) 8.07 and negative LR of 0.21. A DI cut-off value of 0.29 predicted a difficulty of ET with a sensitivity of 88,9% and a specificity of 85%.ConclusionsCervical ultrasound elastography, by allowing the identification of cervical tissue dishomogeneity, may be of help in predicting the ET ease in infertile women candidates to IVF/ICSI.

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Factors associated with patency of the uterine cervix in bitches with pyometra

Cited by 9 publications

References 38 publications

Collagen and Glycosaminoglycan Profiles in the Canine Cervix during Different Stages of the Estrous Cycle and in Open- and Closed-Cervix Pyometra

Collagen and Glycosaminoglycan Profiles in the Canine Cervix during Different Stages of the Estrous Cycle and in Open- and Closed-Cervix Pyometra

Canine pyometra: a model for the analysis of serum CXCL8 in inflammation

Evaluation of the cervix tissue homogeneity by ultrasound elastography in infertile women for the prediction of embryo transfer ease: a diagnostic accuracy study

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