Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

Characterization of the Aminoacylation of Cytoplasmic Transfer Ribonucleic Acids from Germinating Soybeans

“…( Figure 3). No inhibition was detected by increasing protein concentrations as reported by others (1,20).…”

“…Novelli (15) emphasized the importance of determining the optimal conditions with regard to ionic strength, pH, (20) found that a ratio of 8 was optimal for a mixture of amino acids for the Phaseolus leaf system, while Burkard et al (6) obtained maximum leucylation at a ratio of 1.5 for the same system. The soybean system has one maximum peak of leucyl synthetase activity ( Figure 7) with variations in the concentrations of Mg^"â nd ATP (with an optimal Mg^^/ATP ratio).…”