Factors controlling embryo viability

Hartshorne, Geraldine M.

doi:10.1080/1464727012000199571

Cited by 4 publications

(2 citation statements)

References 96 publications

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“…Furthermore, the result also implicates that telomerase and its components may play only a partial role in embryo development. In fact, not only telomerase, but also many other diverse factors, including nutrition, embryo metabolism, culture/coculture system, gamete formation, embryonic genome activation, and imprinting, are known to affect embryo viability and development (31). More comprehensive and informative assessments of an embryo are needed to explore and clarify the behavior of early human embryos in vitro.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of Human Telomerase Reverse Transcriptase mRNA Expression in Individual Blastomeres as an Indicator of Early Embryo Development

Wang

Yao

Horng

et al. 2004

J Assist Reprod Genet

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Purpose : The study was undertaken to test whether human telomerase reverse transcriptase (hTERT) transcripts in an individual blastomere could be used as an indicator for embryo development. Methods : Group A consisted of day 3 normal cleaving embryos at 4-to 8-cell stage, which were surplus and not allocated for uterine transfer. Group B consisted of arrested or fragmented embryos at the same stage, which were considered to be compromised. After blastomere dissociation, RNA purification, reverse transcription, and hTERT PCR amplification, the amplified product was separated by 3% gel electrophoresis. Results : Eighty-six (90.5%) of the 95 intact blastomeres in group A and 78 (70.9%) of the 110 blastomeres in group B demonstrated hTERT mRNA expression. The difference was statistically significant (P < 0.05, chi-square). Eight (38.1%) of the 21 embryos in group A and 22 (62.9%) of the 35 embryos in group B had at least one blastomere that did not express hTERT mRNA under this procedure. The difference was not significant (P > 0.05, chi-square). Conclusions : General hTERT mRNA transcripts can be detected in most of the individual blastomeres but cannot be used as an indicator for early embryo development. Further investigations are necessary to elucidate its clinical application.

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Section: Discussionmentioning

confidence: 99%

Feasibility of Human Telomerase Reverse Transcriptase mRNA Expression in Individual Blastomeres as an Indicator of Early Embryo Development

Wang

Yao

Horng

et al. 2004

J Assist Reprod Genet

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“…The morphological criteria do not necessarily correlate with implantation success rates, and approximately 50% of healthy embryos fail to implant [10]. Additionally, several factors are involved in embryogenesis and development [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Association of Polymorphisms in FSHR, INHA, ESR1, and BMP15 with Recurrent Implantation Failure

Shin

Lee

et al. 2023

Biomedicines

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Recurrent implantation failure (RIF) refers to two or more unsuccessful in vitro fertilization embryo transfers in the same individual. Embryonic characteristics, immunological factors, and coagulation factors are known to be the causes of RIF. Genetic factors have also been reported to be involved in the occurrence of RIF, and some single nucleotide polymorphisms (SNPs) may contribute to RIF. We examined SNPs in FSHR, INHA, ESR1, and BMP15, which have been associated with primary ovarian failure. A cohort of 133 RIF patients and 317 healthy controls consisting of all Korean women was included. Genotyping was performed by Taq-Man genotyping assays to determine the frequency of the following polymorphisms: FSHR rs6165, INHA rs11893842 and rs35118453, ESR1 rs9340799 and rs2234693, and BMP15 rs17003221 and rs3810682. The differences in these SNPs were compared between the patient and control groups. Our results demonstrate a decreased prevalence of RIF in subjects with the FSHR rs6165 A>G polymorphism [AA vs. AG adjusted odds ratio (AOR) = 0.432; confidence interval (CI) = 0.206–0.908; p = 0.027, AA+AG vs. GG AOR = 0.434; CI = 0.213–0.885; p = 0.022]. Based on a genotype combination analysis, the GG/AA (FSHR rs6165/ESR1 rs9340799: OR = 0.250; CI = 0.072–0.874; p = 0.030) and GG-CC (FSHR rs6165/BMP15 rs3810682: OR = 0.466; CI = 0.220–0.987; p = 0.046) alleles were also associated with a decreased RIF risk. Additionally, the FSHR rs6165GG and BMP15 rs17003221TT+TC genotype combination was associated with a decreased RIF risk (OR = 0.430; CI = 0.210–0.877; p = 0.020) and increased FSH levels, as assessed by an analysis of variance. The FSHR rs6165 polymorphism and genotype combinations are significantly associated with RIF development in Korean women.

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Microfluidic sperm sorting improves ICSI outcomes in patients with increased values of Double-Strand Breaks in sperm DNA

Lara-Cerrillo

Muñoz²,

Heras³

et al. 2023

Revista Internacional de Andrología

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Factors controlling embryo viability

Cited by 4 publications

References 96 publications

Feasibility of Human Telomerase Reverse Transcriptase mRNA Expression in Individual Blastomeres as an Indicator of Early Embryo Development

Feasibility of Human Telomerase Reverse Transcriptase mRNA Expression in Individual Blastomeres as an Indicator of Early Embryo Development

Association of Polymorphisms in FSHR, INHA, ESR1, and BMP15 with Recurrent Implantation Failure

Microfluidic sperm sorting improves ICSI outcomes in patients with increased values of Double-Strand Breaks in sperm DNA

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