Factors controlling gastric-glucagon release.

Glucagon gene transcription is negatively regulated by insulin in a hamster islet cell line.

“…It is interesting to note that, although the cisacting DNA sequences responsible for the tissue-specific expression of the glucagon gene appear to be different in the A cell of the pancreas compared to the L cell of the intestine (41,42), the same DNA element could mediate insulin effects on glucagon gene regulation in these two tissues. Indeed, insulin effects on glucagon secretion do not appear to be limited to the A cell of the pancreatic islets, but are probably also observed on the canine gastric A cells (31) and the intestinal L cells (43). This model thus offers the possibilities to identify discrete DNA sequences through which insulin exerts negative regulatory effects and the trans-acting factors interacting with them.…”

Vagal stimulation and its role in eliciting gastrin but not glucagon release from the isolated perfused dog stomach.

“…The stomachs of overnight-fasted 9-5-12 kg dogs of both sexes were isolated according to a procedure previously described by Lefebvre and Luyckx (1977) with, in addition, careful dissection and isolation of both dorsal and ventral vagal trunks along the oesophagus to a distance of about 5 cm from the cardia. The isolated stomachs were perfused with whole canine blood supplemented with Trasylol (Bayer, Leverkusen, Germany) as previously described (Lefebvre and Luyckx, 1977).…”

Glucagon and diabetes: A reappraisal

“…Soon after, it was demonstrated that the fundus of the dog stomach contains [18][19][20] and releases [18,[42][43][44] a material identical by all criteria to pancreatic glucagon; the dog stomach also possesses A-cells undistinguishable from the pancreatic A-cells [18]. Thus the pancreatectomized dog does not constitute a model of glucagon deficiency.…”