Factors governing development of nonalcoholic fatty liver disease and insulin resistance in obesity

Abstract

The factors promoting development of non-alcoholic fatty liver disease in patients with obesity and different state of carbohydrate metabolism have been studied. 43 patients were examined; these included 26 patients with abdominal obesity (BMI=52.9±7.9 kg/m2). The control group consisted of 17 conditionally healthy donors without obesity (BMI=18.9-24.9 kg/m2), seven of them formed a comparison group that was included to compare the results of study on the levels of tissue-specific expression of HSP70 mRNA. The study of mRNA expression was performed by real-time PCR. The concentration of IL-6 and TNF-a was measured in blood serum by the ELISA method. In patients with obesity with diabetes mellitus type 2 (DM2), a significant increase in the serum level of proinflammatory cytokines was found in comparison with the group of patients without DM2 and control. The results of histological examination of liver biopsy specimens in obese patients revealed the most pronounced changes in the group of DM2 patients. Regardless of the stage of nonalcoholic fatty liver disease in obese DM2 patients, an increase in the area of fatty inclusions (relative to the group without type 2 diabetes) was recorded. The study of the HSP70 gene expression in peripheral blood mononuclear cells allowed its significant increase relative to the comparison group. The relationship between the level of expression of the HSP70 gene in metabolically active tissues (visceral, subcutaneous adipose tissue and liver) established in all obese patients with the serum content of proinflammatory cytokines (IL-6 and TNF-a) may indicate suppression of HSP70 expression in these tissues, background of systemic and local inflammation in obesity.

Cornus officinalis vinegar reduces body weight and attenuates hepatic steatosis in mouse model of nonalcoholic fatty liver disease

“…Tumor necrosis factor (TNF)‐α has been considered a proinflammatory cytokine responsible for eliciting immunopathology in inflammation (S. Li et al., 2016; Vulf et al., 2018); TNF‐α over‐expression has been observed in NAFLD mouse model (Z. Li et al., 2003). By contrast, interleukin (IL)−10 is one of the anti‐inflammatory cytokines that inhibit the production and release of proinflammatory cytokines in inflammatory sites (Maloy & Powrie, 2011).…”

“…Tumor necrosis factor (TNF)-α has been considered a proinflammatory cytokine responsible for eliciting immunopathology in inflammation (S. Li et al, 2016;Vulf et al, 2018); TNF-α over-expression has been observed in NAFLD mouse model (Z. Li et al, 2003).…”

Heat Shock Proteins (HSPs) and Cardiovascular Complications of Obesity: Searching for Potential Biomarkers

“…In their study on HSP70 expression in various types of cells and tissues of obese patients, Vulf et al established its statistically significant increase in the mononuclear cells of peripheral blood, compared with nonobese individuals. Notably, the expression level of HSP70 was suppressed in visceral subcutaneous adipose tissue and liver, where the authors observed a high expression of proinflammatory cytokines [67].…”

Clinical and laboratory features of the course of non-alcoholic liver steatosis in women in the early postmenopausal period living on the territory of an industrial megapolis

“…В нашем исследовании маркеры ЭД и провоспалительный IL-6 у пациенток со стеатозом и ожирением в раннем периоде постменопаузы были значимо выше по сравнению с группой контроля. Полученные нами данные согласуются с рядом других исследований, которые изучали цитокиновый статус при НАЖБП [24,25].…”