Factors governing substrate-induced generation and extrusion of protons in the yeast Saccharomyces cerevisiae

Sigler, Karel; Knotková, A.; Kotyk, Arnošt

doi:10.1016/0005-2736(81)90353-9

Cited by 78 publications

(26 citation statements)

References 23 publications

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“…5) show that the pH decrease with time was slowed down by the presence of ethanol, effect that was visible both in the slope of the pH curve and in the time required to reach a certain pH value. The explanation may be based on the fact that the acidification process is accomplished with the contribution of many mechanisms, 22,23) such as CO 2 production, organic acid release by cells in the medium, and active proton efflux, and all these processes are slowed down if a delay in growth takes place. The fact that in our experiments the final pH was not influenced by the ethanol concentration is probably due to the much larger buffering capacity of the growth medium we used.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Antoce¹,

Antoce²,

Takahashi³

et al. 1997

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Antoce¹,

Antoce²,

Takahashi³

et al. 1997

Bioscience, Biotechnology, and Biochemistry

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“…level (18,19). It was unexpected that the addition of (NH4)2SO4 would result in such a strong expulsion of protons or that the high corn system would be unable to buffer the additional proton load.…”

Section: And Discussionmentioning

confidence: 99%

Ammonium acceleration of glycolysis in the ethanol fermentation in a high corn system.

Ziffer¹

1986

J. Gen. Appl. Microbiol.

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“…Sacch. cerevisiae was cultured in a synthetic medium according to Sigler et al (1981), Sch. pombe in a minimal medium as described by Gutz et al (1974) which contained 2-5 YO (w/v) glucose as carbon source.…”

Section: Methodsmentioning

confidence: 99%

Response of catalase activity and membrane fluidity of aerobically grown Schizosaccharomyces pombe and Saccharomyces cerevisiae to aeration and, the presence of substrates

Gille

Sigler

Höfer

1993

Journal of General Microbiology

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Intracellular catalase (EC 1.11.1.6) activity of permeabilized aerobically grown cells of Schizosaccharomyces pombe was insensitive to cell aeration and inhibition of protein synthesis, and was only mildly enhanced by the presence of glucose and ethanol via de novo protein synthesis. By contrast, the intracellular catalase activity of Saccharomyces cerevisiae, which, in freshly harvested cells, was two to three times lower than that in Sch. pombe, increased on aeration without substrates or with ethanol and was inhibited on aeration with glucose following cell permeabilization. The enhanced intracellular activity was due to de novo protein synthesis while the inhibitory effect of glucose, absent in Sch. pombe, was caused by one of the major glucose metabolites, succinate. The intactcell catalase activity of both yeasts increased greatly during aeration. In Sacch. cerevisiae, this increase was again prevented by glucose. In parallel, export of catalase to the cell surface increased in both yeasts. This was especially conspicuous in Sch. pombe aerated in the presence of ethanol, and may represent a protective mechanism against the damaging effects of ethanol. The cell-surface-bound catalase activity was confirmed in isolated plasma membranes of both yeasts. The fluidity of the plasma membrane increased during aeration. This effect was further stimulated by the presence of glucose and to a lesser extent by ethanol. Both yeasts exhibited increased extracellular catalase activity during aeration which could not be caused entirely by cell lysis. In Sch. pombe this activity was strongly enhanced by the presence of ethanol.

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Factors governing substrate-induced generation and extrusion of protons in the yeast Saccharomyces cerevisiae

Cited by 78 publications

References 23 publications

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Ammonium acceleration of glycolysis in the ethanol fermentation in a high corn system.

Response of catalase activity and membrane fluidity of aerobically grown Schizosaccharomyces pombe and Saccharomyces cerevisiae to aeration and, the presence of substrates

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