Factors influencing attachment of thermophilic bacilli to stainless steel

Parkar, Shanthi G.; Flint, Steve; Palmer, John W.; Brooks, J.

doi:10.1046/j.1365-2672.2001.01323.x

Cited by 146 publications

(103 citation statements)

References 27 publications

(32 reference statements)

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“…Flint et al (10) showed that attachment of bacteria can be inhibited by whole milk but can also be enhanced by the presence of lactose and noncasein protein solutions. Milk proteins have been found to decrease attachment of both vegetative cells and spores of thermophilic bacilli (27). Our study revealed small amounts of submerged B. cereus biofilms.…”

Section: Discussionmentioning

confidence: 55%

Air-Liquid Interface Biofilms of Bacillus cereus : Formation, Sporulation, and Dispersion

Wijman

Leeuw

Moezelaar

et al. 2007

Appl Environ Microbiol

240

168

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Biofilm formation by Bacillus cereus was assessed using 56 strains of B. cereus, including the two sequenced strains, ATCC 14579 and ATCC 10987. Biofilm production in microtiter plates was found to be strongly dependent on incubation time, temperature, and medium, as well as the strain used, with some strains showing biofilm formation within 24 h and subsequent dispersion within the next 24 h. A selection of strains was used for quantitative analysis of biofilm formation on stainless steel coupons. Thick biofilms of B. cereus developed at the air-liquid interface, while the amount of biofilm formed was much lower in submerged systems. This suggests that B. cereus biofilms may develop particularly in industrial storage and piping systems that are partly filled during operation or where residual liquid has remained after a production cycle. Moreover, depending on the strain and culture conditions, spores constituted up to 90% of the total biofilm counts. This indicates that B. cereus biofilms can act as a nidus for spore formation and subsequently can release their spores into food production environments.

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Section: Discussionmentioning

confidence: 55%

Air-Liquid Interface Biofilms of Bacillus cereus : Formation, Sporulation, and Dispersion

Wijman

Leeuw

Moezelaar

et al. 2007

Appl Environ Microbiol

240

168

View full text Add to dashboard Cite

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“…DSF regulation of exopolysaccharide production may also contribute to virulence and transmission in X. fastidiosa. In some systems, exopolysaccharides inhibit cells from forming initial, specific attachments required for biofilm formation, perhaps by masking attachment proteins (34,35). In other cases, exopolysaccharide synthesis enhances biofilm formation (36,37), perhaps at postattachment steps.…”

Section: Discussionmentioning

confidence: 99%

Cell-cell signaling controlsXylella fastidiosainteractions with both insects and plants

Newman

Almeida

Purcell

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

263

296

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Xylella fastidiosa, which causes Pierce's disease of grapevine and other important plant diseases, is a xylem-limited bacterium that depends on insect vectors for transmission. Although many studies have addressed disease symptom development and transmission of the pathogen by vectors, little is known about the bacterial mechanisms driving these processes. Recently available X. fastidiosa genomic sequences and molecular tools have provided new routes for investigation. Here, we show that a diffusible signal molecule is required for biofilm formation in the vector and for vector transmission to plants. We constructed strains of X. fastidiosa mutated in the rpfF gene and determined that they are unable to produce the signal activity. In addition, rpfF mutants are more virulent than the wild type when mechanically inoculated into plants. This signal therefore directs interaction of X. fastidiosa with both its insect vector and plant host. Interestingly, rpfF mutants can still form in planta biofilms, which differ architecturally from biofilms in insects, suggesting that biofilm architecture, rather than a passive response to the environment, is actively determined by X. fastidiosa gene expression. This article reports a cell-cell signaling requirement for vector transmission. Identification of the genes regulated by rpfF should elucidate bacterial factors involved in transmission and biofilm formation in the insect.

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“…Surface growth causes various negative effects in many technical systems, and different means to eliminate, reduce, and control the formation of biofilms have been suggested (4,11,29,30). One of the areas where problems with biofilm growth are rather severe is in pulp and paper mills.…”

Section: Discussionmentioning

confidence: 99%

N -Acetyl- l -Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

Olofsson¹,

Hermansson

Elwing³

2003

Appl Environ Microbiol

226

192

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N-Acetyl-L-cysteine (NAC) is used in medical treatment of patients with chronic bronchitis. The positive effects of NAC treatment have primarily been attributed to the mucus-dissolving properties of NAC, as well as its ability to decrease biofilm formation, which reduces bacterial infections. Our results suggest that NAC also may be an interesting candidate for use as an agent to reduce and prevent biofilm formation on stainless steel surfaces in environments typical of paper mill plants. Using 10 different bacterial strains isolated from a paper mill, we found that the mode of action of NAC is chemical, as well as biological, in the case of bacterial adhesion to stainless steel surfaces. The initial adhesion of bacteria is dependent on the wettability of the substratum. NAC was shown to bind to stainless steel, increasing the wettability of the surface. Moreover, NAC decreased bacterial adhesion and even detached bacteria that were adhering to stainless steel surfaces. Growth of various bacteria, as monocultures or in a multispecies community, was inhibited at different concentrations of NAC. We also found that there was no detectable degradation of extracellular polysaccharides (EPS) by NAC, indicating that NAC reduced the production of EPS, in most bacteria tested, even at concentrations at which growth was not affected. Altogether, the presence of NAC changes the texture of the biofilm formed and makes NAC an interesting candidate for use as a general inhibitor of formation of bacterial biofilms on stainless steel surfaces.

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Factors influencing attachment of thermophilic bacilli to stainless steel

Cited by 146 publications

References 27 publications

Air-Liquid Interface Biofilms of Bacillus cereus : Formation, Sporulation, and Dispersion

Air-Liquid Interface Biofilms of Bacillus cereus : Formation, Sporulation, and Dispersion

Cell-cell signaling controlsXylella fastidiosainteractions with both insects and plants

N -Acetyl- l -Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

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