The purpose of this research was to understand the factors that influence the level of biosecurity adoption in beef cattle farms. This research was conducted in Soppeng Regency, South Sulawesi Province, Indonesia in 2019. The population was beef cattle farmers. The sample was purposively chosen, totaling 30 persons. The data were collected through observation and in-depth interviews using a questionnaire. The questionnaire consisted of 30 questions about biosecurity practices, namely, sanitation, isolation and traffic control. Each question had two possible choices: not adopted was scored 0 and adopted scored 1. A linear regression model was used to assess the factors influencing level of biosecurity adoption. The results showed that the assessed variables, namely age, education, number of family and number of beef cattle explained 86.7% of the percentage adoption of biosecurity (adjusted R 2); thus, 13.3% were influenced by other factors which were not involved in the model. Simultaneously, all variables affected significantly the level of biosecurity adoption (P < 0.05). Partially, education level and herd size affected significantly the level of biosecurity adoption in beef cattle farms (P < 0.01 and P < 0.05, respectively). It is suggested that extension and motivation for farmers should be enhanced in order to increase biosecurity adoption.