Factors Influencing Death of Biotin-Deficient Yeast Cells

Change of Cell Wall Polysaccharides of Yeast in Biotin Deficiency

“…As already mentioned, cells of the present yeast strain grown in the biotin-suboptimal Am medium fell synchronously into death at a rate of more than 90% by replacing the culture to biotin-free Asp medium (3). In this replaced culture, growth in the sense of an increase in optical density occurred well but cells with bud lost their ability to multiply in any nutritive medium, indicating their death.…”

Levels of Biotin Enzymes During Unbalanced Growth and Death of Biotin-Deficient Yeast Cells

“…Moreover, this death was stimulated by chelating agents and their action seemed to be restrained in the part of cell surface (3).…”

Cell Death Produced by Antibiotic Cerulenin in Biotin-requiring YeastSaccharomyces cerevisiae

“…12 centage of dead cells than in Asp medium without biotin, S. cerevisiae grown on biotin-deficient medium containing ammonium salfate (Am medium) for 32 hr was harvested, washed once with sterile deionized water, and then inoculated into biotin-sufficient medium containing aspartic acid (Asp medium) and various amounts of cerulenin. The Asp medium used here contained 0.002 pg/ml biotin.…”