Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts

Quecini, Vera; Oliveira, Carlos Alberto de; Alves, Adriana; Vieira, Maria Lúcia Carneiro

doi:10.1590/s1415-47572002000100014

Cited by 14 publications

(10 citation statements)

References 52 publications

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“…Gene electrotransfer of plant protoplasts was successfully applied for transformation of several crop species such as maize, rice, wheat, sorghum, soybean, and rye (Fromm et al 1986;Lee et al 1986;Christou et al 1987;Pitt et al 1997;Quecini et al 2002). Cell viability preservation is crucial for production of transgenic plants, as transformed protoplast should maintain the ability of normal organogenesis.…”

Section: Plant Protoplast Electroporationmentioning

confidence: 99%

“…Conflicting reports about electrotransfected plant protoplast regeneration ability are found in the literature. Some authors reported increased cell division, plant regeneration, and DNA synthesis in protoplast transformed by electroporation (Rech et al 1987;Rech et al 1988;Chand et al 1988;Ochatt et al 1988;Joersbo et al 1991), while others found slower plant regeneration of electrotransfected protoplasts (Quecini et al 2002). In some studies, increasing electric filed strength and the number of pulses decreased plant protoplast viability and plating efficiency.…”

Section: Plant Protoplast Electroporationmentioning

confidence: 99%

See 1 more Smart Citation

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

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In this chapter, basics and mechanisms of electroporation are presented. Most important electric pulse parameters for electroporation efficiency for different applications that involve introduction of small molecules and macromolecules into the cell or cell membrane electrofusion are described. In all these applications, cell viability has to be preserved. However, in some biotechnological applications, such as liquid food sterilization or water treatment, electroporation is used as a method for efficient cell killing. For all the applications mentioned above, besides electric pulse parameters, other factors, such as electroporation medium composition and osmotic pressure, play significant roles in electroporation effectiveness. For controlled use of the method in all applications, the basic mechanisms of electroporation need to be known. The phenomenon was studied from the single-cell level and dense cell suspension that represents a simplified homogenous tissue model, to complex biological tissues. In the latter, different cell types and electric conductivity that change during the course of electric pulse application can significantly affect the effectiveness of the treatment. For such a complex situation, the design and use of suitable electrodes and theoretical modeling of electric field distribution within the tissue are essential. Electroporation as a universal method applicable to different cell types is used for different purposes. In medicine it is used for electrochemotherapy and genetherapy. In biotechnology it is used for water and liquid food sterilization and for transfection of bacteria, yeast, plant protoplast, and intact plant tissue. Understanding the phenomenon of electroporation, its mechanisms and optimization of all the parameters that affect electroporation is a prerequisite for successful treatment. In addition to the parameters mentioned above, different biological characteristics of treated cell affect the outcome of the treatment. Electroporation, gene electrotransfer and electrofusion are affected by cell membrane fluidity, cytoskeleton, and the presence of the cell wall in bacteria yeast and plant cells. Thus, electroporation parameters need to be specifically optimized for different cell types.

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Section: Plant Protoplast Electroporationmentioning

confidence: 99%

Section: Plant Protoplast Electroporationmentioning

confidence: 99%

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

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“…As variedades de S. guianensis, apresentam ampla variabilidade relacionada a características de produção, adaptação a solos ácidos e de baixa fertilidade, tolerân-cia à seca e tolerância a pragas e doenças sendo consideradas moderadamente tolerantes à salinidade (Gonzalez et al, 2000;Quecini et al, 2002).…”

Section: Enzymatic Reaction To Saline Stress During Stylosanthes Germunclassified

Reação enzimática ao estresse salino durante a germinação de estilosantes

Gonela

Lemos²,

Rodrigues³

et al. 2004

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a influência do NaCl nos sistemas isoenzimáticos durante o estádio inicial de germinação do Stylosanthes guianensis (Aubl.) Sw. Sementes das variedades botânicas canenscens, microcephala, pauciflora e vulgaris foram colocadas para germinar em caixas de plástico, com papel-filtro umedecido com água (controle) e solução de NaCl (134 mmol), em estufa BOD, por 14 horas. Após este período, foi realizada a extração das enzimas glutamato desidrogenase e peroxidase. As enzimas apresentaram comportamentos diferenciados nas quatro variedades quando submetidas ao estresse salino.Termos para indexação: Stylosanthes guianensis, salinidade, leguminosa forrageira, isoenzimas Enzymatic reaction to saline stress during stylosanthes germinationAbstract -The objective of this work was to evaluate the influence of the NaC1 on isoenzyme systems during the initial stage of germination of Stylosanthes guianensis (Aubl.) Sw. Seeds of the botanical varieties canenscens, microcephala, pauciflora and vulgaris were placed to germinate in plastic boxes, with filter paper moistened with water (control) and NaC1 solution(134 mmol), in BOD greenhouse, for 14 hours. After this period, the extraction of glutamate dehydrogenase ensymes and peroxidase was carried out. The enzymes presented differentiated behaviors in the four varieties when subjected to the saline stress.

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“…In Stylosanthes guianensis, a tropical forage legume, direct DNA uptake using an electroporation-mediated method (3.2 x 10 7 electroporated protoplasts produced 50 regenerants, from which 18 were transgenic) was superior to a biolistic approach (Quecini, 1999;Quecini et al, 2002). The protocol described herein could be used for genetic transformation of alfalfa, and somatic hybridization experiments involving Medicago species.…”

Section: In Vitro Proceduresmentioning

confidence: 99%

“…Therefore the protocol of Atanassov & Brown (1984) was used to obtain somatic embryos and, subsequently, plants from M. sativa 'Rangelander' protoplasts. This system should allow microparticle bombardment and protoplast electroporation, the principal mechanisms for direct gene delivery into legume plant cells (Aragão et al, 1999;Potrykus, 1991;Quecini et al, 2002). Genetic engineering methods are very useful for the transference of disease resistance genes into alfalfa genome in Brazil, namely to control the fungi Rhizoctonia solani and Colletotrichum trifolii.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

Monteiro

Appezzato‐da‐Glória

Valarini

et al. 2003

Sci. agric. (Piracicaba, Braz.)

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Alfalfa is one of the most frequently studied species from the production of tissue culturederived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leafderived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.

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Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts

Cited by 14 publications

References 52 publications

Electroporation in Biological Cell and Tissue: An Overview

Electroporation in Biological Cell and Tissue: An Overview

Reação enzimática ao estresse salino durante a germinação de estilosantes

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

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