Factors influencing ELISA evaluation of transmission of pea seed-borne mosaic virus in infected pea seed : seed-group size and seed decortication

Maury, Yves; Bossennec, Jean-Marie; Boudazin, Geneviève; Hampton, R. O.; Pietersen, G.; Maguire, J. D.

doi:10.1051/agro:19870402

Cited by 24 publications

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“…Belinda having the most probable seed transmission rate of 3.5% (the confidence intePr'd at 0.95 level being 1.81-6.31 %) was taken up for studies. This rate of PSbMV seed transmission as worked out by group testing of seeds in ELISA (MAURY et al 1987) was further verified by individually testing the embryos of 100 seeds in ELISA wherein 5 infected embryos were detected.…”

Section: Methodsmentioning

confidence: 74%

“…The present article deals with certain aspects of latency in seed transmission of PSbMV m a cultivar of pea, as studied under experimental control of all the possible sources of secondary contaminations. It also demonstrates the failure to detect this latent virus in seedlings at an early growth stage and also in seeds even on usmg an excellent antiserum under the conditions of group analysis precedingly defined for assessing seed transmission rate (MAURY et al 1987).…”

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confidence: 99%

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Seed Transmission of Pea Seed‐borne Mosaic Virus in Peas: Early and Late Expression of the Virus in the Progeny

Khetarpal

Maury

1990

Journal of Phytopathology

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A seed lot of pea cv, Belinda was shown to transmit with high frequency a latent strain of Pea seed-borne mosaic virus, weakly detectable in ELISA only 5 weeks after plant growth. This strain did not induce apparent symptoms on plants, was no more detectable in 9 week old plants and showed a reduced multiplication even on retro-inoculating healthy pea plants; contrarily, the common strain could be easily detected in the seed and young seedlings on which it induced clearcut symptoms.

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Section: Methodsmentioning

confidence: 74%

mentioning

confidence: 99%

Seed Transmission of Pea Seed‐borne Mosaic Virus in Peas: Early and Late Expression of the Virus in the Progeny

Khetarpal

Maury

1990

Journal of Phytopathology

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“…The ELISA procedures developed in the present investigation should be useful in seed-health testing programmes. Similar ELISA tests have also proved helpful in the detection of seed borne viruses such as bean common mosaic (Jafarpour et ai, 1979), lettuce mosaic (Jafarpour et al, 1979;Falk, 1983;Van Vuurde& Maat, 1983), pea seedborne mosaic (Hamilton & Nichols, 1978;Maury et al, 1987;Varma et al, 1991), squash mosaic (Nolan & Campbell, 1984) and soybean mosaic (Lister, 1978;Maury et a/., 1983).…”

Section: Discussionmentioning

confidence: 99%

Influence of the amount of blackgram mottle virus in different tissues on transmission through the seeds of Vigna mungo

1992

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Blackgram mottle virus (BMoV) was transmitted via up to 16% of seeds of different cultivars of blackgram, as determined by seedling symptom tests. The percentage of seed infection by BMoV as determined by EL1SA was even higher. Seed transmission was highest in cv. PLU‐277 (15.9%), followed by cvs T‐9 (11.8%), PLU‐213 (7.0%) and UH‐81‐7 (1.3%). Seed transmission was correlated with the amount of virus present in the embryonic axis and later in primary leaves. The presence of virus in the testa alone did not result in its transmission through seeds. Virus concentration in different tissues varied; the mean amount of virus in the three cultivars was found to be 48–1234 ng per embryonic axis, 15–24 ng per cotyledon, and 12–20 ng per testa. The infection of primary leaves through the seed also resulted in systemic infection if the amount of virus in primary leaves exceeded 100 ng/100 mg of tissue. Close agreement was found between the percentage of seedlings with systemic infection and the percentage of seeds and embryonic axes containing more than 100 ng virus. The cultivars that resisted seed transmission contained relatively small amounts of the virus in embryonic axes.

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“…If the OD 405 nm distribution of the different healthy samples is considered to be normal and if we select the coefficient in a t distribution table (Student Fisher) by which the standard deviation (s) has to be multiplied to give a value which has a 0.005 probability to be exceeded, then the healthy limit on a given microplate is: x + 3.5 s when referring to eight healthy values (x being the average OD 405 nm value) [12].…”

Section: Serological Testsmentioning

confidence: 99%

Spread of cymbidium mosaic potexvirus and potyviruses in vanilla plants grown in shade houses in Reunion Island

Quillec

Rivière

Lagorce

2001

Fruits

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-Introduction. Beginning in 1994, vanilla vines grown under shade houses in Reunion Island have developed disorders mainly due to the cymbidium mosaic potexvirus (CymV) and potyviruses. To reduce damage and avoid viral epidemics, we considered developing a vanilla nursery to supply farmers with virus-free plant material and to provide prophylactic advice. Accordingly, we determined the relationship between symptoms on vanilla leaves and viral diagnosis. We also determined the temporal and spatial distribution patterns of viral diseases. Materials and methods. Symptom assessment and serological tests were carried out on vanilla leaves (Vanilla fragrans) at various times. The relationship between symptoms and viral infection data was analyzed with Statistica software. Spatial distribution patterns of infected plants were generated with two-dimensional distance class analysis (2DClass). Results and discussion. Results showed that symptoms do not correlate well with serological diagnosis. Plants in all shade houses in the survey were infected mainly by CymV which spreads in the row according to cultural practices. Potyviruses could be transmitted both by insect vectors and mechanically, but for the latter case less efficiently than CymV. Conclusion.Results from this study can be used to develop control measures, including the production of healthy planting materials to manage plant disease epidemics. Further studies will involve the characterization of vanilla potyviruses so that reliable serological techniques and better molecular tools can be developed. Reunion / Vanilla fragrans / disease surveys / protected cultivation / potexviruses / potyviruses / symptoms / disease transmission / epidemiologyDissémination sous ombrière du cymbidium mosaic potexvirus et de potyvirus affectant les lianes de vanilliers cultivés à l'île de la Réunion.Résumé -Introduction. Depuis 1994, le dépérissement du vanillier, cultivé en système intensif sous ombrière à l'île de la Réunion, est principalement dû au « cymbidium mosaic virus » (CymV) et aux potyvirus. Pour limiter les dégâts et mieux contrôler l'épidémie virale, nous avons étudié la relation entre la présence de symptômes et le diagnostic viral ainsi que la dynamique temporelle et spatiale des virus sous ombrière. Ces études ont été utilisées pour apporter de judicieux conseils prophylactiques et mettre en place une pépinière de boutures de vanillier afin de fournir aux planteurs du matériel végétal indemne de virus. Matériel et méthodes. L'observation des symptômes et le diagnostic sérologique ont été réalisés à partir de feuilles de vanille (Vanilla fragrans) à l'occasion de différentes prospections sous ombrières. La relation entre l'expression des symptômes et les données d'infection virale a été analysée avec le logiciel Statistica. La distribution spatiale des lianes infectées a été étudiée par la méthode d'analyse des classes de distance en deux dimensions (2Dclass). Résultats et discussion. Les résultats montrent que l'expression des symptômes n'est pas systém...

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Factors influencing ELISA evaluation of transmission of pea seed-borne mosaic virus in infected pea seed : seed-group size and seed decortication

Abstract: et al.. Factors influencing ELISA evaluation of transmission of pea seed-borne mosaic virus in infected pea seed : seed-group size and seed decortication. Agronomie, EDP Sciences, 1987, 7 (4)

Cited by 24 publications

References 0 publications

Seed Transmission of Pea Seed‐borne Mosaic Virus in Peas: Early and Late Expression of the Virus in the Progeny

Seed Transmission of Pea Seed‐borne Mosaic Virus in Peas: Early and Late Expression of the Virus in the Progeny

Influence of the amount of blackgram mottle virus in different tissues on transmission through the seeds of Vigna mungo

Spread of cymbidium mosaic potexvirus and potyviruses in vanilla plants grown in shade houses in Reunion Island

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