Aim: Hand hygiene is critical for the prevention of healthcare-associated infections, yet it is poorly practiced by healthcare workers. The aim of this study is to examine the effects of various incentives on the correctness of hand disinfection technique and motivation to use the technique correctly. Participants and Methods: The experiment was performed on three different days with 36 healthcare students. The procedure on the first and third day was the same and equal for all four groups (feedback alone). On the second day, the groups received different incentives (1: feedback + demonstration + creative teamwork; 2: feedback + demonstration + lecture; 3: feedback + demonstration; 4: feedback alone). Correctness (gel coverage percentage) and motivation were measured and compared between the three measurements. Results: Initially, only 25% of participants achieved at least 95% coverage, and only 55.56% tried at least "quite a bit" to use the technique correctly. Coverage increased after the incentive in groups 1 (χ2(2) = 6.000; P = 0.050), 2 (χ2(2) = 7.750; p = 0.021), and 3 (χ2(2) = 15.273; P = 0.001). Motivation increased in groups 1 (χ2(2) = 10.571; P = 0.005) and 3 (χ2(2) = 7.515; P = 0.023). Dorsum coverage was significantly smaller than palm coverage in both hands. Conclusion: Demonstration of hand disinfection technique, creative teamwork, and lecture were effective in adopting the habit of hand disinfection and reinforcing motivation to do so, but scanner feedback alone wasn't effective in this way. Future studies should examine longer follow-up period and testing these results in clinical settings.