Factors influencing <i>Ralstonia pseudosolanacearum</i> infection incidence and disease development in rose plants

Wolf, J.M. van der; Kastelein, P.; Poleij, L.M.; Krijger, M.C.; Mendes, O.; Sedighian, Nasim; Allen, Cattlyn; Bonants, P.J.M.; Kurm, Viola

doi:10.1111/ppa.13596

Cited by 2 publications

(2 citation statements)

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“…Temperature had a strong effect on the infection of R. solanacearum, incidence and development of wilt symptoms in many crops (Bittner et al 2016;Singh et al 2014;Tjou-Tam-Sinet al 2017;van der Wolf et al 2022). There are reports which corroborated that the severity of the bacterium increased in areas where the temperature ranged from 24 to 35 °C (Lemay et al 2003) as temperature has been found to be a crucial governing factor in the pathogenesis of the disease, interaction between host and parasite, reproduction and survival of the pathogen in the soil.…”

Section: Discussionmentioning

confidence: 99%

Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan

Aslam

Mukhtar

2023

Bragantia

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The production of chili is seriously threatened by bacterial wilt incited by Ralstonia solanacearum in Pakistan. As the information about the distribution and prevalence of the disease and the pathogen is scarce, the present study was performed to ascertain the prevalence, occurrence and distribution of R. solanacearum in different regions of chili cultivation. The results showed differences in the incidence and prevalence of bacterial wilt all over the country. Overall, an incidence of 10% and prevalence of 76% of the disease was observed in the country. The highest incidence of bacterial wilt was observed in the province of Sindh whereas it was the lowest in Baluchistan. The prevalence of the disease followed the similar trend in the provinces. As regards agroecological zones, the highest incidence was recorded in Indus delta followed by sandy deserts whereas it was the lowest in western dry mountains. Almost the same tendency was observed regarding prevalence in the eight agroecological zones. In case of districts, the disease incidence was the maximum in Badin while it was the minimum in the district of Loralai. On the other hand, the disease prevalence was the maximum (100%) in the districts of Badin, Mirpurkhas, and Thatta and the minimum (60%) in the districts of Barkhan, Karak, and Loralai. Of the total 114 isolates of R. solanacearum, 81% were confirmed as Biovar 3 whereas the rest 19% isolates were identified as Biovar 4. The findings will help the farmers to design disease management programs accordingly to avert yield losses.

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Section: Discussionmentioning

confidence: 99%

Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan

Aslam

Mukhtar

2023

Bragantia

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“…2.1 Bacterial strain and growth conditions R. solanacearum phylotype II (race 3 biovar 2) strain IPO-4187 was received from the working collection at Wageningen UR and used in this study. Strain IPO-4187 was derived by making R. solanacearum strain PD2762, isolated from potatoes in the Netherlands in 2016, rifampicin resistant following the protocol described by van der Wolf et al (2022). The strain belongs to sequevar 1 (R. solanacearum phylotype IIB-1) (Anonymous, 2018).…”

Section: Methodsmentioning

confidence: 99%

Dose-response relationship of Ralstonia solanacearum and potato in greenhouse and in vitro experiments

Eisfeld

Schijven²,

Kastelein³

et al. 2022

Front. Plant Sci.

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Ralstonia solanacearum is the causative agent of bacterial wilt of potato and other vegetable crops. Contaminated irrigation water contributes to the dissemination of this pathogen but the exact concentration or biological threshold to cause an infection is unknown. In two greenhouse experiments, potted potato plants (Solanum tuberosum) were exposed to a single irrigation with 50 mL water (non-invasive soil-soak inoculation) containing no or 102 – 108 CFU/mL R. solanacearum. The disease response of two cultivars, Kondor and HB, were compared. Disease development was monitored over a three-month period after which stems, roots and tubers of asymptomatic plants were analyzed for latent infections. First wilting symptoms were observed 15 days post inoculation in a plant inoculated with 5x109 CFU and a mean disease index was used to monitor disease development over time. An inoculum of 5x105 CFU per pot (1.3x102 CFU/g soil) was the minimum dose required to cause wilting symptoms, while one latent infection was detected at the lowest dose of 5x102 CFU per pot (0.13 CFU/g). In a second set of experiments, stem-inoculated potato plants grown in vitro were used to investigate the dose-response relationship under optimal conditions for pathogen growth and disease development. Plants were inoculated with doses between 0.5 and 5x105 CFU/plant which resulted in visible symptoms at all doses. The results led to a dose-response model describing the relationship between R. solanacearum exposure and probability of infection or illness of potato plants. Cultivar Kondor was more susceptible to brown-rot infections than HB in greenhouse experiments while there was no significant difference between the dose-response models of both cultivars in in vitro experiments. The ED50 for infection of cv Kondor was 1.1x107 CFU. Results can be used in management strategies aimed to reduce or eliminate the risk of bacterial wilt infection when using treated water in irrigation.

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Factors influencing Ralstonia pseudosolanacearum infection incidence and disease development in rose plants

Cited by 2 publications

References 30 publications

Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan

Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan

Dose-response relationship of Ralstonia solanacearum and potato in greenhouse and in vitro experiments

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