Factors influencing seminal plasma composition and its relevance to succeed sperm technology in sheep: An updated review

Moula, Anass Ben; Amiri, Bassir

doi:10.1016/j.smallrumres.2022.106759

Cited by 5 publications

(3 citation statements)

References 121 publications

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Section: Discussionmentioning

confidence: 90%

“…In the same study, it was reported that after 8 h of storage, progressive motility declined in sperm extended in Duragen® compared to the one extended in SM (Benmoula et al, 2018). In this regard, given the difference in seminal plasma composition, it was highlighted that the conservation process can differ between breeds within species and even within males from the same breed (Benmoula & El Amiri, 2022). Furthermore, our study revealed that during 8 h of storage, velocity parameters (VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) did not vary significantly between the two extenders, whereas after 24 h, the VCL, VAP, LIN and WOB decreased significantly only in SM extender.…”

Section: Discussionmentioning

confidence: 99%

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Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination

El Amiri,

Ben Moula,

El Khelfaoui

et al. 2023

Reprod Domestic Animals

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This study aimed to investigate the effectiveness of Duragen® and skimmed milk (SM) extenders on the quality parameters, bacterial load and fertilization ability of stored ram semen. A total of 50 ejaculates from Sardi rams (n = 5) aged 2.5–3 years, were collected and stored in Duragen® and SM at 15°C. The motilities and velocity parameters generated by the CASA system were then evaluated at 0, 8 and 24 h of storage. Afterward, bacterial loads of sperm extended in Duragen® and SM were determined at 0, 5 and 24 h of incubation. In addition, ewes (n = 100) aged 2 years, have been chosen in the same herd. The selected ewes were then synchronized and inseminated using semen extended in Duragen® and SM and stored for 5 h at 15°C. The results revealed that total and progressive motilities, straight velocity (VSL), straightness (SRT), lateral head displacement (ALH) and beat cross frequency (BCF) were not affected by the extender type after 24 h of storage (p > .05). However, curvilinear velocity (VCL), velocity average path (VAP), linearity (LIN) and wobble (WOB) showed higher values in Duragen® compared with SM extender after 24 h of storage (p < .05). Bacterial loads were observed mainly in sperm stored in SM at 5 h (183 UFC/mL) and at 24 h (357 UFC/mL) of incubation. However, the only case showing a bacterial load in Duragen® is when the storage time attains 24 h (199 UFC/mL). Concerning fertility, sperm diluted in both extenders resulting in high fertility rates which reaches 66% and 73% for Duragen® and SM, respectively, with no statistical difference (p > .05). In summary, Duragen® extender decreased bacterial load in stored semen and maintained high ram sperm quality and fertility. These findings suggest that Duragen® extender could be used as SM alternative in ovine artificial insemination (OAI).

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination

El Amiri,

Ben Moula,

El Khelfaoui

et al. 2023

Reprod Domestic Animals

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show abstract

“…Remarkably, under anaerobic conditions, the maintenance of motility was liable upon the availability of reducing sugar (du Plessis et al., 2015; Gangwar et al., 2020). While fructose occupies the primary role of energy supplier in seminal plasma (Ben Moula & El Amiri, 2022; Macfarlane & Abbiss, 1975), it is worth noting the adaptability of spermatozoa, which exhibit the ability to metabolize alternative carbohydrates in the absence of fructose (Salamon & Maxwell, 2000). Notably, lactose, a prominent milk carbohydrate, serves a dual purpose by preserving osmolarity and furnishing an energy source for spermatozoa (Miyasaki et al., 2014).…”

Section: Discussionmentioning

confidence: 99%

Adjusting ram semen preservation: Exploring the impact of oxygen exposure during liquid storage

Ben Moula,

Hamidallah,

Badi

et al. 2024

Reprod Domestic Animals

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This study investigated the effects of storage conditions on the quality of chilled ram semen stored at 4°C for 48 h, comparing aerobic and anaerobic conditions. Ejaculates from INRA180 rams were collected and stored under both conditions, with assessments at 0‐, 24‐, and 48‐h intervals. Various sperm parameters were examined, including motility, velocity, viability, morphology, membrane integrity, and lipid peroxidation. Results showed that storage duration significantly impacted sperm quality, leading to a gradual decline from 0 to 24 h and 24 to 48 h. Notably, after the initial 24 h, progressive motility (PM) and membrane integrity (MI) demonstrated distinct responses to storage conditions. Anaerobic storage consistently improved PM and MI values compared to aerobic storage between 24 and 48 h. Anaerobic conditions also enhanced viability and reduced abnormality at the 48‐h mark. Total motility remained stable throughout storage. Velocity parameters (VCL: curvilinear velocity; VSL: straight velocity and VAP: velocity average path) exhibited differences between the 24‐ and 48‐h intervals, with anaerobic storage resulting in higher VAP and VSL values. Moreover, lipid peroxidation exhibited a progressive increase from 0 to 24 h and 24 to 48 h, independent of storage conditions. Remarkably, anaerobic storage consistently yielded lower lipid peroxidation levels compared to aerobic storage, regardless of storage duration. In conclusion, this study highlights that the anaerobic storage proved advantageous for chilled ram semen quality, particularly after the initial 24 h.

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Heat stress and ram semen production and preservation: Exploring impacts and effective strategies

Ben Moula,

Moussafir,

Hamidallah

et al. 2024

Journal of Thermal Biology

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Factors influencing seminal plasma composition and its relevance to succeed sperm technology in sheep: An updated review

Cited by 5 publications

References 121 publications

Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination

Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination

Adjusting ram semen preservation: Exploring the impact of oxygen exposure during liquid storage

Heat stress and ram semen production and preservation: Exploring impacts and effective strategies

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