Factors influencing substrate utilization by Tetrahymena pyriformis

Corbett, John J.

doi:10.1016/0014-4827(58)90099-5

Cited by 11 publications

(5 citation statements)

References 7 publications

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“…Cell volume was calculated on the basis of cell shape as approximating that of a prolate spheroid (1). Average values were derived from measurements of 20 cells from each sample and cell volume data were analyzed by the group comparison method (30).…”

Section: Methodsmentioning

confidence: 99%

Effect of Type of Carbohydrate on Growth and Protein Synthesis by Tetrahymena pyriformis

Reynolds

Wragg

1962

The Journal of Protozoology

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SYNOPSIS. In chemically defined media at carbohydrate concentrations ≧ 0.5% (w/v) Tetrahymena pyriformis W multiplied more rapidly, developed larger cells, and achieved greater growth as measured by optical density when carbohydrate was provided as dextrin rather than glucose. In media containing 0.3 mg/ml of amino acid nitrogen, growth increased with glucose concentration from 0.1 to 1%, did not change significantly to 3%, and was sharply inhibited at higher glucose levels. With dextrin, maximum growth paralleled carbohydrate concentration from 0.1 to 3%. At higher N levels the inhibitory concentration of glucose was lowered, but growth in dextrin media was not affected except at N concentrations that were inhibitory independent of carbohydrate source. At 1% carbohydrate levels, total cell protein per ml of culture was 60% greater, protein per cell approximately 50% greater, and cells were 1.5 to 2 times larger in media with dextrin than with glucose. Comparable differences in protein synthesis were observed at 2% carbohydrate levels and efficiency of conversion of substrate‐N to protein‐N was greater in the medium with dextrin than glucose. Growth as measured by optical density in media with 0.3 mg/ml of N and 1 or 2% (w/v) of dextrin was not significantly reduced by the simultaneous presence of 1 or 2% glucose. This observation appeared to negate osmotic pressure as an explanation of reduced growth in the presence of glucose. At higher osmolar concentrations osmotic pressure appeared to be a major determinant of overall growth but not of cell size.

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Section: Methodsmentioning

confidence: 99%

Effect of Type of Carbohydrate on Growth and Protein Synthesis by Tetrahymena pyriformis

Reynolds

Wragg

1962

The Journal of Protozoology

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“…Cell counts were made with a haemocytometer on diluted cell suspensions that were inactivated with phosphate-buffered formaldehyde. Cell volume was calculated from the measurements of cell length and width on the basis of cell shape as approximating that of a prolate spheroid (Corbett, 1958). Average values were derived from measurements of 20 cells from each sample.…”

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confidence: 99%

Free Amino Acids in Serine-Antagonized Cells of Tetrahymena pyriformis

1965

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Growth inhibition of Tetrahymena pyriformis by L-serine in a chemically defined medium was reversed by L-arginine in a manner which resembled competitive antagonism. Composition of the free amino acid pools from cells grown in either a balanced amino acid mixture or a mixture with serine concentrations which inhibited growth suggested an antagonism by serine with energy-yielding reactions. Growth in media with excess serine resulted in the accumulation of higher concentrations of free cellular amino acids and an apparent increase in the rate of conversion of arginine to ornithine, as compared with growth in the balanced medium. The results suggested that serine or a metabolic product of serine interferes with the formation of pyruvic acid. In the presence of high levels of serine, arginine appeared to be metabolized more rapidly and to be spared when alanine, aspartic acid, or glutamic acid was added to the unbalanced medium.

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“…Single Tetrahymena are unable to survive and grow in the chemically defined medium unless it is supplemented with 0.04% PP or yeast nucleic acids (7,16). Transfer of a larger number of cells brings about sufficient "adjustment of the medium" to permit survival (8).…”

Section: Resultsmentioning

confidence: 99%

Enrichment for Auxotrophic Mutants of Tetrahymena Using Density Gradient Centrifugation*

Hepfer

Kaney

1980

The Journal of Protozoology

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43 1 netic modification, in Golden R, Pollard T, Rosenbaum J , eds., Cell Motility, Book C, Microtubular and Related Proteins. Cold Spnng Harbor Conferences on Cell Proliferation 3, 941-8. 7. Moreton RB, Amos WB. 1979. Electrical recording from the contractile ciliate Zoothamnium geniculatum Ayrton. J . Exp. Biol. 83, 8. Nuccitelli R, Po0 MM, Jaffe LF. 1977. Relations between ameboid movement and membrane-controlled electrical events. J . Gen. Physiol. 69, 743-63. 9. Riddle J. 1962. Studies on the membrane potential of Pelomyxa carolinensis. Exp. Cell Res. 26, 15867. 10. Shiono H, Hara R, Asai H. 1980. Spontaneous membrane po-159-67. tential changes associated with the zooid and vacuolar contractions in Vorticella convallaria. J . Protozool. 27, 83-7. 11. Spoon DM. 1976. Use of thin, flexible plastic coverslips for microscopy, microcompression, and counting of aerobic microorganisms. Trans. Am. Microsc. Soc. 95, 520-3. 12. -1979. Physiological studies on stalkless suctona. Trans. Am. Microsc. Soc. 98, 1W1. 13. -, Chapman GB, Cheng RS, Zane SF. 1976. Observations on behavior and feeding mechanisms of the suctorian Heliophrya erhardi (Reider) Metthes preying on Paramecium. Trans. Am. Microsc. SOC. 95, 443-62.

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Factors influencing substrate utilization by Tetrahymena pyriformis

Cited by 11 publications

References 7 publications

Effect of Type of Carbohydrate on Growth and Protein Synthesis by Tetrahymena pyriformis

Effect of Type of Carbohydrate on Growth and Protein Synthesis by Tetrahymena pyriformis

Free Amino Acids in Serine-Antagonized Cells of Tetrahymena pyriformis

Enrichment for Auxotrophic Mutants of Tetrahymena Using Density Gradient Centrifugation*

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