Factors Influencing the Adherence of Pseudomonas aeruginosa to Mammalian Buccal Epithelial Cells

Woods, Donald E.; Straus, David C.; Johanson, W. G.; Bass, Joseph A.

doi:10.1093/clinids/5.supplement_5.s846

Cited by 163 publications

(182 citation statements)

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“…In severely ill patients, excess proteolytic enzyme activity in oral secretions cleaves fibronectin from cell surfaces [24], thus potentiating adherence of Gram-negative bacteria to buccal epithelial cells and in this way promoting colonization of the oropharynx [25]. In the present investigation, salivary fibronectin digestive activity exhibited no apparent relationship with either CMV infections or oropharyngeal colonization by Gram-negative bacilli.…”

Section: D)iscufssioncontrasting

confidence: 58%

Relationship between cytomegalovirus and colonization of the oropharynx by Gram-negative bacilli following renal transplantation

et al. 1991

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SUMMARYNumerous investigators have reported an increased incidence of pneumonia caused by Gram-negative bacilli and other secondary pathogens in transplant recipients infected by cytomegalovirus (CMV). To determine if CMV infections are related to colonization of the upper respiratory tract by Gram-negative bacilli, we examined prospectively 22 renal transplant recipients with sequential bacteriological, virological and biochemical examinations performed just prior to and at various times after transplantation. Only 11 % of subjects had Gram-negative bacilli isolated from gargle specimens prior to transplantation, as compared to 54 % after transplantation. More importantly, after transplantation, subjects with active CMV infections were more likely to have prolonged oropharyngeal carriage of Gram-negative bacilli than subjects without CMV infections (36 % v. 25 %). During active CMV infections, the rate at which Gram-negative bacilli were isolated from gargle specimens rose from 28 to 47 %. During culture-positive CMV infections, the isolation rate reached 57 % and was significantly different from that of CMV-negative samples (P < 0O01). The increased rate of Gram-negative bacillary isolation from gargle specimens during CMV infections was not a function of type of immunosuppresive agents used, rejection episodes, antibiotic administration, concomitant hepatitis B, Epstein-Barr (EBV) virus, or herpes simplex virus infections, or alterations in salivary fibronectin concentrations.

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Section: D)iscufssioncontrasting

confidence: 58%

Relationship between cytomegalovirus and colonization of the oropharynx by Gram-negative bacilli following renal transplantation

et al. 1991

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“…Under our assay conditions, non-piliated P. aeruginosa mutants do not bind cells (Woods et al, 1980), despite the fact that such strains bind mucins (Ramphal et al, 1991b). O-linked mucin carbohydrates have been implicated in P. aeruginosa flagellum-mediated binding (Ramphal & Arora, 2001), which may play a later role, particularly within the accumulated mucus of the CF airway.…”

Section: A405mentioning

confidence: 85%

Laboratory and clinical Pseudomonas aeruginosa strains do not bind glycosphingolipids in vitro or during type IV pili-mediated initial host cell attachment

Emam

Park

et al. 2006

Microbiology

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The glycosphingolipids (GSLs) gangliotriaosylceramide (Gg3) and gangliotetraosylceramide (Gg4) have been implicated as receptors for type IV pili (T4P)-mediated Pseudomonas aeruginosa epithelial cell attachment. Since P. aeruginosa T4P are divided into five groups, the authors determined whether GSLs in general, and Gg3 and Gg4 in particular, are specifically bound and required for host epithelial cell attachment of clinical and laboratory strains within these groups. An enterohaemorrhagic Escherichia coli strain, CL56, known to bind to both Gg3 and Gg4, provided a positive control. TLC overlay showed no binding of more than 12 P. aeruginosa strains to either Gg3 or Gg4 (or other GSLs), while CL56 Gg3/Gg4 binding was readily detectable. GSL ELISA similarly demonstrated no significant P. aeruginosa binding to Gg3 or Gg4, compared with CL56. Using a selective chemical inhibitor, epithelial cell GSL synthesis was abrogated, and Gg3 and Gg4 expression deleted, but P. aeruginosa attachment was not impaired. Target cell attachment was mediated by T4P, since non-piliated, but flagellated, mutants were unable to bind to the target cells. CFTR (cystic fibrosis transmembrane conductance regulator) has also been implicated as a receptor; however, in this work, overexpression of CFTR had no effect on P. aeruginosa binding. It is concluded that neither Gg3 nor Gg4 are specifically recognized by P. aeruginosa, and that endogenous GSLs do not have a role in the attachment of live intact P. aeruginosa to cultured lung epithelial cells. In contrast to whole piliated P. aeruginosa, T4P sheared from such bacteria showed significant Gg3 and Gg4 binding, which may explain the results of other studies.

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“…Fibronectin, a glycoprotein present in extracellular matrix and at the surface of epithelial cells, is also hydrolysed by HLE (McDonald et al, 1979;Suter et al, 1988). The proteolytic activity of saliva from severely ill patients has been shown to be significantly correlated with the increased adherence of P. aeruginosa to buccal cells in uitro, due to fibronectin degradation (Woods et al, 1980a(Woods et al, , 1983.…”

Section: Introductionmentioning

confidence: 99%

Adherence of Pseudomonas aeruginosa to respiratory epithelium and the effect of leucocyte elastase

Plotkowski

Beck²,

Tournier³

et al. 1989

Journal of Medical Microbiology

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Summary. The tracheobronchial secretions from patients with cystic fibrosis often contain high amounts of free proteases. To evaluate whether human leucocyte elastase (HLE) can favour the persistence of bacterial airways infection, we exposed the frog palate mucosa to HLE and then to radiolabelled Pseudomonas aeruginosa and followed the sequence of events by scanning electronmicroscopy. In response to HLE there was a marked outpouring of mucus and a desquamation of the epithelium. P. aeruginosa was shown to adhere to recently secreted granules of mucus and to the exposed submucosal underlying connective tissues. For the eight different bacterial strains studied, a significative adherence to HLE-injured mucosa was observed only in strains that possessed internal haemagglutinating activity. Neither the presence of fimbriae, nor of the mucoid exopolysaccharide, nor of the bacterial surface haemagglutinating activity could be related to adherence of P. aeruginosa to the injured mucosa. These results support the hypothesis that HLE enhances bacterial infection of the respiratory mucosa both by inducing mucus hypersecretion and by exposing receptors to the microbial adhesins. It is also suggested that P. aeruginosa internal lectins may be implicated in adherence to host tissues.

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Factors Influencing the Adherence of Pseudomonas aeruginosa to Mammalian Buccal Epithelial Cells

Cited by 163 publications

References 0 publications

Relationship between cytomegalovirus and colonization of the oropharynx by Gram-negative bacilli following renal transplantation

Relationship between cytomegalovirus and colonization of the oropharynx by Gram-negative bacilli following renal transplantation

Laboratory and clinical Pseudomonas aeruginosa strains do not bind glycosphingolipids in vitro or during type IV pili-mediated initial host cell attachment

Adherence of Pseudomonas aeruginosa to respiratory epithelium and the effect of leucocyte elastase

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