This is an investigation of the current situation of post-traumatic stress disorder (PTSD) among operating room nurses in large comprehensive hospitals and provide a scientific basis for managers to formulate effective intervention measures. In total, 266 operating room nurses were randomly selected from 6 large comprehensive hospitals in Zhejiang Province via a multi-stage random sampling method. The self-designed general information questionnaire and Chinese version of the PTSD self-rating scale (PCL-C) were used for the investigation. According to the PCL-C scores, the nurses were divided into two groups: ≥ 41 was divided into a PTSD group and < 41 was divided into a non-PTSD group. A single factor comparison was performed on the general data line and then a multivariate logistic analysis was conducted. The total score of the PCL-C was 45.41 ± 9.46 (95%CI 44.27–46.55), including an avoidance numbness symptom score of 19.13 ± 4.65 (95%CI 18.57–19.69), a re-experience symptom score of 12.82 ± 3.37 (95%CI 12.41–13.23), and a high vigilance symptom score of 13.46 ± 3.52 (95%CI 13.03–13.88). A univariate and multivariate logistic analysis showed there were significant differences in age, marital status, working years, professional titles, positions and shift arrangements between the two groups (P < 0.05). The PTSD incidence among operating room nurses in large comprehensive hospitals is relatively high. Factors such as age, marital status, working years, professional title, position, and shift scheduling significantly impacted their PTSD incidence. Therefore, managers can formulate effective intervention measures based on these results to reduce the incidence of PTSD among operating room nurses.