Factors influencing the induction of endo-xylanase by Thermoascus aurantiacus

Gomes, Donald James; Gomes, Joseph; Steiner, Walter

doi:10.1016/0168-1656(94)90101-5

Cited by 41 publications

(22 citation statements)

References 30 publications

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“…However, decline in enzyme production at higher wheat bran levels (4.5 % w/v) could be due to the formation of a thick suspension and improper mixing of the substrate in shake fl asks fl [25][26] . Reduced synthesis of enzyme in high concentration of xylan containing lignocellulosic material could also be due to the accumulation of more reducing sugars 27 .…”

mentioning

confidence: 99%

Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology

et al. 2007

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Response surface methodology employing central composite design (CCD) was used to optimize fermentation medium for the production of cellulase-free, alkaline xylanase from Streptomyces violaceoruber under r submerged fermentation. The design was employed by selecting wheat bran, peptone, beef extract, incubation time and agitation as model factors. A second-order quadratic model and response surface method showed that the optimum conditions for xylanase production (wheat bran 3.5 % (w/v), peptone 0.8 % (w/v), beef extract 0.8 % (w/v), incubation time 36 h and agitation 250 rpm) results in 3.0-fold improvement in alkaline xylanase production (1500.0 IUml -1 ) as compared to initial level (500.0 IUml -1 ) after 36 h of fermentation, whereas its value predicted by the quadratic model was 1347 IUml -1 . Analysis of variance (ANOVA) showed a high coefficient of determination (R fi 2 ) value of 0.9718, ensuring a satisfactory adjustment of the quadratic model with the experimental data.The economical and cellulase-free nature of xylanase would enhance its applicability in pulp and paper industry.

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mentioning

confidence: 99%

Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology

et al. 2007

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“…Xylanase secretion and its extracellular performance are known to depend directly on the type of ions present in solution (Kaupinen et al 1997). Gomes et al (1994) recorded a similar observation on the production of xylanase by Thermoascus aurantiacus. At a high concentration of metal ions, enzyme production was low, which could be due to the blockage of the secretion of protein into the external medium (Shaku et al 1980).…”

Section: Xylanase Production (Ulmentioning

confidence: 67%

Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans

Sharma

Adhikari

Satyanarayana

2006

World J Microbiol Biotechnol

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The optimization of cultural variables resulted in a marked enhancement in the secretion of cellulase-free and alkali-thermostable xylanase (EC 3.2.1.8) by an extreme thermophile Geobacillus thermoleovorans. The enzyme secretion was enhanced when the medium was supplemented with xylan (0.15%) and Tween-80 (0.1% v/v). In wheat brantryptone medium, the peak in enzyme production was attained within 42 h in a fermenter as compared to 72 h in shake flasks. Optimization of the culture conditions resulted in a 7.72-fold enhancement in enzyme production. The cellulase-free xylanase was optimally active at pH 8.5 and 80°C, and it was found to be useful in the pre-bleaching process of paper pulps.

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“…Surfactants may promote the release of cellbound enzymes but are also likely to increase membrane permeability. The activity of various surfactants (Tween 80, Tween 20, Triton X-100) on the yield of cell-wall degrading enzymes (β-glucosidase, endoglucanase, exoglucanase, manganese peroxidase) by various fungi has been described frequently (Gomes et al 1994;Pushalkar et al 1995;Rodriguez et al 1998;Pardo and Forchiassin 1999). On the other hand, though we cannot exclude that Tween compounds may be used in part as carbon sources themselves (Caldwell et al 1991), Tween 80 did not appear to sustain much T. matsutake growth in a carbon-deficient agar medium (Guerin-Laguette et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants

et al. 2002

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Saprotrophic growth of Tricholoma matsutake isolates was investigated over Pinus densiflora bark fragments either on soil or on agar media. Preferential colonization of pine bark fragments by hyphae, in glucose-deprived environments suggested that Matsutake was able to extract some nutrients to sustain its growth. This was confirmed in glucose-free liquid nutrient medium, where bark as sole carbon source significantly stimulated (up to two-fold) growth of T. matsutake isolates. The addition of surfactants (Tween 80 and Tween 40) in liquid medium further stimulated mycelium growth over pine bark by up to 55%. Such growth stimulation was associated with a sharp increase in protein and beta-glucosidase excretion by hyphae in culture filtrates. As T. matsutake has some saprotrophic ability, the initiation and extension of Matsutake Shiro in forest soil might require simultaneously nutrients derived from the host plant and from soil organic compounds. Data reported here may contribute to the formulation of new culture substrates adapted to the co-culture of T. matsutake and its host plant under controlled conditions.

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Factors influencing the induction of endo-xylanase by Thermoascus aurantiacus

Cited by 41 publications

References 30 publications

Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology

Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology

Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans

Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants

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