Factors Influencing the Proliferation and Differentiation of Brain Neurons In Vitro

Aizenman, Yair; Wu, Doris K.; Vellis, Jean de

doi:10.1007/978-1-4613-2037-1_20

Cited by 3 publications

(1 citation statement)

References 57 publications

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“…However, the presence of 0.1% trypsin inhibitor did not reduce the concentration of insulin required. It is also unlikely that the high dosage of insulin required is acting through insulin-like growth factor receptors as previously thought (Aizenman et al, 1986b), since the effect of insulin-like-growth factor (AmGen) could not be consistently reproduced. Heparin has been shown to reduce the effective dosage of ECGF in mediating its biological activities in target cells (Wagner and D'Amore, 1986;Schreiber et al, 1985).…”

Section: Resultsmentioning

confidence: 94%

Regulation of neuroblast proliferation by hormones and growth factors in chemically defined medium

Wu¹,

Maciag

Vellis

1988

Journal Cellular Physiology

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Pure neuronal cultures prepared from 6-day-old embryonic chick brains incorporated [3H]-thymidine in serum-free medium up to the 4th day in culture. The addition of insulin any time within this culture period caused an increase in thymidine incorporation. This increase in [3H]-thymidine was correlated with an increase in cell number and percentage of labeling index. Triiodothyronine and endothelial cell growth factor were also active in stimulating [3H]-thymidine incorporation into chick neuroblasts. The effect of these trophic agents is unique since a variety of known mitogens tested were negative.

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Section: Resultsmentioning

confidence: 94%

Regulation of neuroblast proliferation by hormones and growth factors in chemically defined medium

Wu¹,

Maciag

Vellis

1988

Journal Cellular Physiology

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Glutamine synthetase gene experession in a glioblastoma cell-line of clonal origin: Regulation by dexamethasone and dibutyryl cyclic AMP

et al. 1995

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We investigated the expression of glutamine synthetase (GS), an enzyme involved in astroglial metabolism and marker of astroglial functional maturity, in a glioblastoma cell-line (GL-15) of clonal origin. In spite of their phenotypic immaturity, evidenced in a mosaic fashion by a poor glial fibrillary acidic protein (GFAP) expression, the level of GS-mRNA is high in GL15 cells and the considerable amount of GS biological activity can be further induced and stabilized by glucocorticoids. A correlation between the induction by dexamethasone of the GS-mRNA level and the GS biological activity suggests a transcriptional regulation of GS expression by the aforesaid hormone. Under this hormonal action, changes in cell morphology occur and they are correlated with an overexpression of the GFAP, a marker of astroglial differentiation. On the contrary, dibutyryl cyclic AMP (dbc AMP) down-regulates the GS-mRNA expression and decreases GS activity. These results suggest that GL-15 cells have a common glucocorticoid dependent mechanism able to induce GS and GFAP as well as morphological changes. However in these cells AMPc responsive elements are involved in the negative modulation of the GS expression, contrary to what occurs in normal astroglial cells.

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Brain neurons develop in a serum and glial free environment: effects of transferrin, insulin- insulin-like growth factor-I and thyroid hormone on neuronal survival, growth and differentiation

1987

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Factors Influencing the Proliferation and Differentiation of Brain Neurons In Vitro

Cited by 3 publications

References 57 publications

Regulation of neuroblast proliferation by hormones and growth factors in chemically defined medium

Regulation of neuroblast proliferation by hormones and growth factors in chemically defined medium

Glutamine synthetase gene experession in a glioblastoma cell-line of clonal origin: Regulation by dexamethasone and dibutyryl cyclic AMP

Brain neurons develop in a serum and glial free environment: effects of transferrin, insulin- insulin-like growth factor-I and thyroid hormone on neuronal survival, growth and differentiation

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