Factors influencing the spatio-temporal distribution of benthic Microcystis aeruginosa colonies (Cyanobacteria) in the hypertrophic Grangent Reservoir (Loire, France)

Abstract

The spatio-temporal distribution of benthic colonies of Microcystis aeruginosa in Grangent Reservoir (France) in 2000 was not homogeneous and appeared to be controlled by many external factors: lake depth, station morphometry, substratum and hydraulic regime (lacustrine or fluvial). A most important concentration of benthic colonies was found at deep sites with fine sediment or at sites where the sediment was rich in organic matter. In spite of a stable water level and a minimum flow during summer, the number of benthic colonies showed great variation in the lacustrine downstream part of the reservoir. These variations may be explained by the dynamics of planktonic cyanobacteria. To cite this article: D. Latour, H. Giraudet, C. R. Biologies 327 ( 2004).  2004 Published by Elsevier SAS on behalf of Académie des sciences. RésuméFacteurs influençant la distribution spatio-temporelle des colonies benthiques de Microcystis aeruginosa (Cyanobactérie) sur la retenue de Grangent (Loire, France). La distribution spatio-temporelle des colonies benthiques de Microcystis aeruginosa sur la retenue de Grangent (France) en 2000 n'a pas été homogène et semble être contrôlée par de nombreux facteurs externes : profondeur du lac, morphométrie de la station, nature du substrat et régime hydraulique (fluvial ou lacustre). Les plus fortes concentrations de colonies benthiques ont été trouvées dans les sites les plus profonds avec du sédiment fin ou aux sites dont le sédiment est riche en matière organique. Malgré la stabilité du niveau de l'eau et un débit minimum pendant la période estivale, le nombre de colonies benthiques varie de façon importante dans la zone lacustre, à l'aval de la retenue. Ces variations peuvent en partie être expliquées par la dynamique des colonies planctoniques.

Cyanobacteria Community and Growth Potential Test in Sediment of Lake Paldang

“…휴지기 상태의 조류는 계절변 화와 수온상승 시 휴지기를 마치고 영양세포로 성장하면서 표층으로 부유하여 (Yamamoto and Nakahara, 2009), 식물플 랑크톤 군집을 형성하고, 조건에 따라 대발생을 일으키기도 한다 (Li et al, 2013). 퇴적층에 존재하는 Microcystis 휴면 세포의 현존량은 수체로 부유하여 성장하는 Microcystis 의 규모를 결정하는 것으로 연구되었으며 (Latour and Giraudet, 2004), Anabaena의 휴면포자 역시 성장에 적합한 환경조건 이 갖추어지면 발아 후 성장하여 대량발생할 수 있기 때문에 퇴적층에 존재하는 휴면포자의 현존량이 잠재적인 Anabaena 의 발생량이 된다 (Kim et al, 2015).…”

Application of plow-tillage as an innovative technique for eliminating overwintering cyanobacteria in eutrophic lake sediments

“…It was then filtered with 50-mm mesh sieves to isolate the colonies from the sediment (Latour and Giraudet, 2004). Cyanobacterial colonies isolated from the sediment were dispersed into single cells using an ultrasonicator (JY88, Scientz, China) for cell enumeration.…”

A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria (Microcystis) in lake sediments

“…In theory, the lower detection limit of the reported protocol for benthic Microcystis is 3.3 × 10 4 cells/g dw (as up to 30 mg of dry sediment could be analyzed per run). This suggests that this protocol may be applicable in the analysis of benthic Microcystis in similar eutrophic freshwaters (Boström et al, 1989;Tsujimura et al, 2000;Latour and Giraudet, 2004;Misson et al, 2011).…”

“…For cell enumeration, a small part of the sediment was dried at 105°C to determine the water content, thereafter, the cell concentration of benthic Microcystis was determined with CytoSense following the optimized protocol. For colony analysis, the sediment was filtered with 50 μm mesh sieves to isolate Microcystis colonies (Latour and Giraudet, 2004). In some cases, more than several hundred milliliters of wet sediment were required to obtain a sufficient amount of colonies.…”

“…Traditionally, optical microscopy has been used as a routine facility for quantitative analysis of benthic Microcystis (Brunberg and Blomqvist, 2003;Latour and Giraudet, 2004;Misson et al, 2011). However, there are two recognized shortcomings with this conventional method: (1) it is time-consuming and dependent on skilled personnel; and (2) the number of small colonies and single cells can be underestimated (Tsujimura et al, 2000).…”