Factors Influencing the Success of Sperm‐Cervical Mucus Interaction in Patients Exhibiting Unexplained Infertility

Aitken, R. John; Warner, Pamela; Reid, Clare

doi:10.1002/j.1939-4640.1986.tb00857.x

Cited by 51 publications

(23 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies have shown that these parameters are necessary for in vitro fertilization in rats [31] and boars [32]. In addition, it is worth noting that these parameters are markers of sperm strength, cervical mucus penetration and effective rate of fertilization necessary as the sperm transverse the female reproductive tract with a view of fertilizing the oocyte [33,34] . During the incubation period, there was no observed significant change on LIN and STR, however, there indicators of sperm swimming pattern was increased in the DIO rat after 120 min of incubation when compared with the control.…”

Section: Discussionmentioning

confidence: 99%

Diet-induced obesity alters kinematics of rat spermatozoa

Oyeyipo

Maartens

Plessis

2015

Asian Pacific Journal of Reproduction

View full text Add to dashboard Cite

A B S T R A C TObjective: To investigate the effect of DIO on the kinematics and viability of spermatozoa in an albino rat model. Methods: Sperm suspensions from normal (Control) and diet-induced obese (DIO) Wistar rats were collected and incubated for various times (30, 60, 120 or 180 min at 37 C). Motility parameters were analyzed with computer-aided sperm analysis (CASA), while viability was assessed by means of a dye exclusion staining technique (eosin/ nigrosin). Results: Results reveal that there was a significant time dependent decrease (P < 0.05) in progressive motility, curvilinear velocity and beat cross-frequency after 60 min, while amplitude of lateral head displacement and sperm viability was significantly reduced (P < 0.05) after 120 min in the DIO group compared to control spermatozoa. Conclusions: These results provided evidence that obesity is detrimental to sperm parameter in rats possibly through increased testicular temperature as a result of a rise in fat deposition.

show abstract

Section: Discussionmentioning

confidence: 99%

Diet-induced obesity alters kinematics of rat spermatozoa

Oyeyipo

Maartens

Plessis

2015

Asian Pacific Journal of Reproduction

View full text Add to dashboard Cite

show abstract

“…However, we may speculate on it. It is known that sperm motility characteristics (15,16), their head shape (17,18), and their acrosomal activity (19) are of major importance in the migration and penetration processes. Therefore, we can assume that normally shaped motile spermatozoa with large nuclear vacuoles will probably be able to pass through the female tract barriers and proceed to fertilize the female egg.…”

Section: Figurementioning

confidence: 99%

Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37°C?

Pe’er

Eltes

Berkovitz

et al. 2007

Fertility and Sterility

View full text Add to dashboard Cite

“…In recent studies of sperm-mucus interaction in vitro, a multivariate statistical approach has been applied to data from objective measures of both semen quality and mucus penetration [Aitken et al, 1985[Aitken et al, , 1986Mortimer et al, 19861. The numbers of sperm entering mucus have been shown to depend on the numbers of progressively motile sperm in the semen, as well as on the progressive or straight-line swimming velocity and the amplitude of lateral head displacement.…”

Section: The Cervical Mucusmentioning

confidence: 99%

“…However, sperm in media devoid of enzymes are capable of penetrating mucus, although intrinsic sperm enzymes themselves are not believed to facilitate mucus penetration [Beyler and Zaneveld, 19791. External shearing of the interface in vitro [Katz et al, 19871, designed to model the effects of pressure gradients in vivo [Fox et al, 19701, also increases mucus permeability to sperm.In recent studies of sperm-mucus interaction in vitro, a multivariate statistical approach has been applied to data from objective measures of both semen quality and mucus penetration [Aitken et al, 1985[Aitken et al, , 1986 Mortimer et al, 19861. The numbers of sperm entering mucus have been shown to depend on the numbers of progressively motile sperm in the semen, as well as on the progressive or straight-line swimming velocity and the amplitude of lateral head displacement.…”

mentioning

confidence: 99%

Factors regulating mammalian sperm migration through the female reproductive tract and oocyte vestments

1989

View full text Add to dashboard Cite

Mechanisms of mammalian sperm migration through the female reproductive tract and ovum vestments are described. The perspective is biophysical as well as biochemical and morphological, and the focus is upon the role of sperm motility in these processes. Sperm forward progression is characterized as an interactive process between the the cell and its environment, and the mediation of flagellar bend propagation by the physical properties of its surroundings is described. These properties, together with flagellar beat kinematics, sperm morphology, and surface properties, determine the magnitude of the forces generated by sperm and their consequent rate of progression. Sperm interactions with the cervical mucus, the cumulus oophorus, and the zona pellucida are described. The poorly understood affinity of the sperm surface for the macromolecules of the mucus, cumulus, and zona is stressed, as is the viscoelastic structural mechanical resistance of these biopolymers to sperm motion. The kinematics and consequences of hyperactivated sperm motion are presented, with emphasis on objective characterization of such motion (as a biomarker), along with analysis of the mechanical advantage that such motion may confer on spermatozoa during egg-vestment interaction. Key words: sperm, transport, mucus, cumulus, zona pellucida, fertilization INTRODUCTIONFlagellar movement is a fundamental expression of the vitality of a sperm cell, and is essential for its reproductive function. The active mechanism of flagellar motion produces a sequence of principal and reverse bends [Gibbons and Gibbons, 1974; Woolley, 19771. These can have varying degrees of curvature and can be initiated and propagated along the flagellum at different rates. Flagellar bending results in a distribution of local forces and torques against the surrounding fluid. In accordance with Newton's Third Law, these are resisted by equal and opposite local forces and torques. In order to satisfy Newton's Second Law, the summation of the local local external forces and torques over the sperm head and tail must be zero. As a consequence, there Received November 8, 1988; accepted November 17, 1988. Address reprint requests to David F. Katz In a general biophysical sense, mammalian sperm swim optimally; the principles of hydrodynamics and optimal control theory demonstrate that the smooth flagellar traveling waves, commonly seen in low-viscosity media, tend to maximize the sperm's propulsive velocity with respect to the hydrodynamic power output (Pironneau and Katz, 1974). Mammalian sperm must use their swimming ability to reach the oocyte-cumulus complex, and the forces due to flagellar motion assist them in penetrating the egg vestments to reach the oolemma.Our studies of these processes are hampered by their complexity and by apparent variation among species. Some of this biological variation is real; for example, the site of semen deposition may be the vagina, cervix, or uterus. These environments differ not only with respect to their distance from the site of ferti...

show abstract

Factors Influencing the Success of Sperm‐Cervical Mucus Interaction in Patients Exhibiting Unexplained Infertility

Cited by 51 publications

References 27 publications

Diet-induced obesity alters kinematics of rat spermatozoa

Diet-induced obesity alters kinematics of rat spermatozoa

Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37°C?

Factors regulating mammalian sperm migration through the female reproductive tract and oocyte vestments

Contact Info

Product

Resources

About