Factors Influencing Yield and Neuronal Differentiation of Mesenchymal Stem Cells from Umbilical Cord Blood and Matrix

Rezniczek, Günther A.; Kumbruch, Sandra; Scheich, Jan; Jensen, Arne; Tempfer, Clemens B.

doi:10.2217/rme-2016-0023

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…The variation in the migration of cells from the CT and proliferation rate had no correlation with the type of birth, age, or baby weight. This is similar to the result of Rezniczek et al [24] as they also observed that maternal and gestational age did not influence MSC yield but birth weight did. Two studies observed maternal age influenced CD105+ cell yield but they did not have results on overall cell yield [25,26].…”

Section: Discussion Msc's Isolation Efficiency and Characteristicssupporting

confidence: 91%

Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors

Raileanu

Whiteley

Chow

et al. 2019

Stem Cells Translational Medicine

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Mesenchymal stromal cells (MSCs) have emerged as candidate cells with therapeutic potential to treat different pathologies. The underlying mechanism is paracrine signaling. The cells secrete proteins that can impact inflammation, apoptosis, angiogenesis, and cell proliferation. All are important in wound healing and tissue regeneration. Although the bone marrow has been the most widely used source of MSCs, umbilical cord tissue (CT) presents a source that is just starting to be used in the clinic, yet can be obtained with more ease and easily stored. Here, we characterize CT‐MSCs obtained from multiple donors by analyzing cell surface proteins, differentiation capacity, and proteome profile. Analysis of low, medium, and high passage cells indicates that the morphology and proliferation rate stay constant and with the exception of cluster of differentiation (CD) 105 at late passage, there are no changes in the cell surface protein characteristics, indicating the population does not change with passage. TNF‐stimulated gene 6 protein was measured in a subset of samples and variable expression was observed, but this did not impact the ability of the cells to enhance skin regeneration. In conclusion, CT‐MSC represents a consistent, easily accessible source of cells for cell therapy. Stem Cells Translational Medicine 2019;8:1041–1054

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Section: Discussion Msc's Isolation Efficiency and Characteristicssupporting

confidence: 91%

Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors

Raileanu

Whiteley

Chow

et al. 2019

Stem Cells Translational Medicine

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show abstract

“…According to [30], the yield of MSCs was not signi cantly affected by the mother's age or gestational age, but a greater birth weight was associated with a more yield, which is consistent with our ndings. On the other hand, [24] found that lower birth weight correlates to higher SOX2 gene expression in WJ-SCs.…”

Section: Discussionsupporting

confidence: 91%

“…According to [30], vaginal and cesarean births had MSC cell yields that were comparable. According to [29], the method of delivery had no impact on the characteristics or survivability of MSCs.…”

Section: Discussionmentioning

confidence: 99%

Maternal and Neonatal Factors' Effects on Wharton's Jelly Mesenchymal Stem Cell Yield

Mahmoud,

Bassiouny,

Badawy

et al. 2024

Preprint

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Because Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are easily accessible, easy to isolate, and ethically acceptable, they represent a talented source of MSCs for use in regenerative medicine. Considering decisions on WJ-MSCs collection requires extensive knowledge of the factors that impact their yield. This study's objective was to evaluate the influence of parameters related to mothers and newborns on the WJ-MSCs yield. The WJ-MSCs were isolated and expanded after being taken from 79 umbilical cord samples. Population doubling time and cell proliferation were assessed. By flow cytometry analysis, WJ-MSCs were identified by positivity of CD105, CD90, and CD73 and negativity of CD45 and CD34. There was a statistically significant negative correlation between umbilical cord width and P1 doubling time. Maternal age and WJ-MSC yield were shown to be negatively correlated. Birth weight and gestational age showed a significant positive correlation between WJ-MSCs yield and neonatal variables. No significant correlations were detected between the WJ-MSCs and the mother parity, nor the neonatal sex, fetal presentation, or head circumference. The WJ-MSCs yield increases with younger maternal age, increased gestational age, and increased neonatal birth weight. Hence, consideration should be given to these factors when selecting the ideal donors.

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Influence of preadipocyte-conditioned medium on the proliferation and invasive potential of breast cancer cell lines in vitro

Jabłonka

Scheich

Jacobsen

et al. 2018

Arch Gynecol Obstet

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Factors Influencing Yield and Neuronal Differentiation of Mesenchymal Stem Cells from Umbilical Cord Blood and Matrix

Abstract: Cell yield is affected by certain donor characteristics. hUCB- and WJ-derived MSCs may serve distinct therapeutic niches.

Cited by 6 publications

References 20 publications

Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors

Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors

Maternal and Neonatal Factors' Effects on Wharton's Jelly Mesenchymal Stem Cell Yield

Influence of preadipocyte-conditioned medium on the proliferation and invasive potential of breast cancer cell lines in vitro

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