Factors involved in the production of plantlets from date palm callus cultures

Tisserat, Brent

doi:10.1007/bf00028323

Cited by 50 publications

(26 citation statements)

References 13 publications

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“…Segundo Boulay (1984), elevadas concentrações de carvão ativado podem até mesmo impedir o enraizamento. Embora Tisserat (1982) tenha constatado que a utilização de carvão ativado estimulou o enraizamento, restaurou a capacidade embriogênica in vitro das culturas velhas e diminuiu a intoxicação causada pelos fenóis, os resultados obtidos no presente trabalho não constataram tais efeitos benéficos para o crescimento radicular. Resultados semelhantes ao comportamento do comprimento do sistema radicular podem ser observados para massa fresca de plântulas (Figura 3) e número de folhas (Figura 4).…”

Section: Resultsunclassified

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Chagas¹,

Pasqual²,

Ramos³

et al. 2005

Ciênc. agrotec.

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RESUMOAdição de carvão ativado e giberelina no meio de cultura podem proporcionar melhores condições no desenvolvimento de embriões imaturos de citros. Objetivou-se avaliar o efeito de carvão ativado e GA 3 (ácido giberélico) no cultivo de embriões imaturos provenientes do cruzamento entre laranjeira Pêra Rio x tangerineira Poncã . Após 118 dias da polinização, frutos imaturos, com 3 a 4 cm de diâmetro, foram coletados, suas sementes removidas e tratadas com álcool (70%) por cinco minutos, hipoclorito de sódio (2%) por 20 minutos e, posteriormente, lavadas três vezes em água destilada e autoclavada. Em condições assépticas, os tegumentos das sementes foram separados, os embriões globulares excisados e inoculados em tubos de ensaio contendo 15 mL do meio MT, acrescido de carvão ativado (0; 0,5; 1; 1,5 e 2 g L . Maior comprimento da parte aérea foi obtido em meio MT, acrescido de 0,1 e 1 mg L -1 de GA 3 , combinado com 2 g L -1 de carvão ativado. Maior comprimento do sistema radicular, massa da matéria fresca e número de folhas de plântulas foram obtidos em meio MT, acrescido de 0,01 mg L -1 de GA 3 , na ausência de carvão ativado. A adição de carvão ativado influenciou na concentração de ácido giberélico acrescido no meio de cultura. Termos para indexação:Citrus sinensis, Citrus reticulata, cultura de embriões. ABSTRACTActivated charcoal and gibberelin provides better conditions on development of citrus immature embryos. Activated charcoal and GA 3 (gibberelic acid) on Pêra Rio sweet orange x Poncã mandarin immature embryos culture was evaluated. After 118 dayspollination, imature fruits with 3 to 4 cm of diameter were collected, seeds removed and treated with alcohol (70%) for five min., sodium hypoclorite (2%) for 20 min. and three times washed with distilled and autoclaved water. In aseptic conditions, the teguments were separated, the globular embryos excised and inoculated in test tubes containing 15 mL of the MT medium culture, added of activated charcoal (0; 0.5; 1; 1.5 and 2 g L -1 ) and GA 3 (0; 0.01; 0.1; 1 and 10 mg L -1 ). After inoculation, the embryos were maintained for 90 days in growth room at 27±1ºC, 16 hours photoperiod and 32 mmol m -2 s -1 irradiance. Higher length of the aerial part was obtained in MT medium added 0.01 and 1 mg L -1 of GA 3 combined with 2 g L -1 of activated charcoal. Higher length of the roots system, fresh mass and number of leaves of seedlings was obtained in MT medium added 0.01 mg L -1 of GA 3 and activated charcoal absence. Addition of activated charcoal influenced in giberellic acid concentration added in MT medium.

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Section: Resultsunclassified

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Chagas¹,

Pasqual²,

Ramos³

et al. 2005

Ciênc. agrotec.

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“…Embryogenic calli of date palm were treated with cryogen and stored for several months under ultra-low temperature (-196 ºC). Tisserat (1982) reported date palm regeneration from latent calli revived after 4-8 weeks. The successful cryopreservation of date palm callus after 4 months at -25 ºC was achieved by Mater (1987) by treating with cryoprotectant mixture.…”

Section: Cryopreservationmentioning

confidence: 99%

“…Since the early reports of date palm in vitro regeneration (Tisserat, 1979(Tisserat, , 1982 numerous researchers have described different approaches to achieve date palm micropropagation: somatic embryogenesis (AlKhayri, 2003(AlKhayri, , 2005Fki et al, 2003Fki et al, , 2011aMazri et al, 2017;Al-Khayri, 2016;Othmani et al, 2009a;Roshanfekrrad et al, 2017) and organogenesis (Bekheet, 2013;Jazinizadeh et al, 2015;Bi, 2012;Khierallah;Bader, 2007;Meziani et al, 2015Meziani et al, , 2016. In addition to providing a means for rapid clonal propagation of date palm (Khierallah;Bader, 2007), tissue culture techniques can be utilized for the production of synthetic seeds , cell suspension culture (Othmani et al, 2009b), cryopreservation (Fki et al, 2013), somaclonal variation introduces stress tolerant, disease resistance and with high quality fruits El Hadrami, 2009;Jain, 2001), the production of secondary metabolites (ElSharabasy, 2004) and commercial production of date palm gives considerable profit to both public-sector and private agencies (Zaid;El-Korchi;Visser, 2011).…”

Section: Introductionmentioning

confidence: 99%

Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature inflorescences of adult trees were utilized as an alternative nondestructive source of explants. In addition to the nature of the explant used, successful plant regeneration depends on the cultivar, composition of the culture medium and physical status. Challenges of date palm micropropagation include long in vitro cycle, latent contamination, browning, somaclonal variation as well as ex vitro acclimatization and transplanting. A remarkable amount of research investigating these factors has led to optimized protocols for the micropropagation of numerous commercially important cultivars. This has encouraged the development of several international commercial tissue culture laboratories. Molecular characterization provides an assurance of genetic conformity of regenerated plantlets, a key feature for commercial production. This article describes date palm micropropagation protocols and also discusses recent achievements with respect to somaclonal variation, molecular markers, cryopreservation and future prospects.Index terms: Cryopreservation; somatic embryogenesis; somaclonal variation; organogenesis; molecular marker. RESUMOA tamareira (Phoenix dactylifera L.) é uma arvore frutífera adaptada à condições climáticas adversas predominantemente em regiões áridas do Oriente Médio e Norte Africano. As tâmaras possuem vários componentes químicos com alto valor medicinal e nutricional. A propagação tradicional por estacas não é suficiente para satisfazer a demanda por mudas e assim, a micropropagação apresenta-se como uma alternativa eficiente para a produção de mudas em larga escala. Embriogênese somática e organogênese, tanto direta quanto indireta via calos, tem sido usada para obter a regeneração in vitro de tamareira. O inicio do cultivo in vitro normalmente utiliza meristemas excisados de brotações jovens. Recentemente, inflorescências imaturas de árvores adultas são usadas como fonte alternativa de explantes não destrutiva. Além da origem do explante, o sucesso da regenerção depende do cultivar, da composição do meio de cultura e de condições físicas. Desafios na micropropagação de tamareira incluem um longo ciclo in vitro, contaminação, escurecimento do tecido, variação somaclonal além do enraizamento e aclimatização ex vitro. Diversos estudos investigando esses fatore...

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“…Propagation by seeds cannot be used for the commercial production of elite genotypes due to its heterozygous character [5], and because of the considerable difference between seedlings and vegetatively propagated plants in terms of production potential, fruit maturation and quality, and harvesting time [6]. Propagation by offshoots is a slow technique that is hampered by the restricted number of offshoots produced by a single date palm tree, the low survival rate and the risk of transmitting diseases [7].…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Date Palm: A Review

Mazri¹,

Meziani²

2015

Cell Dev Biol

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Somatic embryogenesis and organogenesis are the two pathways of choice for rapid and large-scale propagation of date palm. They have been successfully used for the micropropagation of elite genotypes and have proved their effectiveness for the commercial production of many cultivars. Nevertheless, regeneration through somatic embryogenesis or organogenesis in date palm is still difficult to achieve for recalcitrant genotypes and is often hampered by certain physiological disorders. In the present review, we report the results of a number of studies carried out on date palm micropropagation. It also describes different factors that influence each stage of somatic embryogenesis and organogenesis and the main problems encountered during these two micropropagation processes.

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Factors involved in the production of plantlets from date palm callus cultures

Cited by 50 publications

References 13 publications

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Date palm micropropagation: Advances and applications

Micropropagation of Date Palm: A Review

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