Factors involved in vivo and in vitro maturation of canine oocytes

Luvoni, G.C.; Chigioni, S.; Allievi, E.; Macis, Debora

doi:10.1016/j.theriogenology.2004.03.004

Cited by 91 publications

(73 citation statements)

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“…Despite the genetic and physiologic usefulness of animal model, studies of transgenic dogs have lagged behind those using laboratory rats and mice as experimental models of human disease. Reasons for the poor development of canine transgenic techniques include the scarcity of available canine oocytes and lack of a canine in vitro maturation system (24). In the present study, we generated two transgenic cloned puppies.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a canine model of human type 2 diabetes mellitus by overexpressing phosphoenolypyruvate carboxykinase

Jeong¹,

Lee

Kim³

et al. 2012

International Journal of Molecular Medicine

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Abstract. Dogs are useful models for studying human metabolic diseases such as type 2 diabetes mellitus due to similarities in physiology, anatomy and life styles with humans. Somatic cell nuclear transfer (SCNT) facilitates the production of transgenic dogs. In this study, we generated transgenic dogs expressing the phosphoenolpyruvate carboxykinase (PEPCK) gene, which is closely involved in the pathogenesis of type 2 diabetes mellitus. In addition, we assessed the cloning efficiency associated with adult or fetal (cloned or natural mating) fibroblasts as a nuclear source. Cloning efficiency was determined by the fusion, pregnancy and cloning rates. The fusion rates were significantly high for fibroblasts from cloned fetuses, but the pregnancy and cloning rates were relatively high for cells from normal fetuses. Based on these data, fetal fibroblasts were selected as the nuclear donor for SCNT and genetically engineered to overexpress the PEPCK gene and dual selection marker genes controlled by the PEPCK promoter. The transgenic cells were introduced into oocytes and transferred into five recipient dogs, resulting in two pregnancies. Finally, three puppies were born and confirmed by microsatellite analysis to be genetically identical to the donor. One puppy successfully overexpressed PEPCK mRNA and protein in the liver. This canine disease model may be useful for studying the pathogenesis and/or therapeutic targets of type 2 diabetes mellitus.

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Section: Discussionmentioning

confidence: 99%

Establishment of a canine model of human type 2 diabetes mellitus by overexpressing phosphoenolypyruvate carboxykinase

Jeong¹,

Lee

Kim³

et al. 2012

International Journal of Molecular Medicine

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“…In contrast to the majority of mammals, canine oocytes are ovulated at the prophase of the first meiotic division (germinal vesicle, GV) and complete the maturation process to metaphase II stage (MII) 48-72 h after ovulation in the oviduct (Hewitt & England 1999, De los Reyes et al 2011. High degeneration (O50%) and very low MII maturation rates (16.2G4.2%) (Luvoni et al 2005, Rodrigues & Rodrigues 2010 are the main features of current IVM in the dog. Dog oocytes contain abundant lipid droplets that occupy 80-90% of the visible ooplasm surface (Guraya 1965, Tesoriero 1982, Songsasen et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free medium

Salavati¹,

Ghafari²,

Zhang³

et al. 2012

Reproduction

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Canine oocytes require an extended period of culture (72 h) in vitro for nuclear maturation to the metaphase II stage, which also results in high degeneration. Canine cumulus oocyte complexes were isolated by slicing from ovaries collected after ovariohysterectomy and cultured in serum-free synthetic oviductal fluid incubated at low (5%) or high (20%) oxygen levels. Changes in oocyte nuclear maturation rates, H 2 O 2 levels within the oocytes and mRNAs of reactive oxygen species inhibitory genes superoxide dismutase 1 and 2 (SOD1 and 2), glutathione reductase (GSR), glutathione peroxidase (GPX1), and catalase (CAT) were quantified. Higher meiotic resumption from germinal vesicle breakdown up to MII was observed in low O 2 (41.8G13.1%) compared to high O 2 (15.8G8.2%) (PZ0.014) after 52 h of culture (nZ112). Extension of the culture period up to 84 h at low O 2 (nZ457 oocytes) produced the highest meiotic resumption at 72 h (64.1G6.0%; PZ0.008), compared with 52 h. Oocytes (nZ110) cultured in high O 2 contained higher levels of peroxidase measured using the 2 0 ,7 0 -dichlorodihydrofluorescein diacetate fluorescence assay after 72 h of culture compared with low O 2 (PZ0.004). High O 2 -cultured oocytes also showed higher amounts of SOD1, SOD2, GSR, GPX1, and CAT mRNA. Vitamin E in high oxygen level was able to decrease degeneration (PZ0.008) but had no improving effect on percentage of oocytes in MII. These results for the first time showed that low oxygen gas composition improves nuclear maturation rates and alleviates the oxidative stress for canine oocytes during in vitro maturation.

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“…However, in dogs in vitro maturation (IVM) of oocytes and IVF is difficult to achieve. Nevertheless capacitated dog spermatozoa are able to penetrate immature oocytes, inducing chromatin decondensation and resumption of meiosis (Luvoni et al, 2005;Hay et al, 1994;Sain-Dizier et al, 2001). Thus, in dogs both, immature or mature oocytes, may be use for this test.…”

Section: Oocyte Penetration Assaymentioning

confidence: 99%