Factors related to follicular oxidative stress in intracytoplasmic sperm injection cycles and its effects on granulosa cells

Abstract

Summary The aim of the present study was to investigate several common conditions that may potentially be correlated with follicular oxidative status during an intracytoplasmic sperm injection (ICSI) cycle and that include the serum oestrogen level on the day of oocyte pick-up, maternal age and pregnancy outcome. Patients that were enrolled in the study were classified randomly into three groups using their numerical order. The first group were classified based on maternal age (<35 and ≥35 years) (n = 398), the second group on the serum oestradiol (E2) level on the day of human chorionic gonadotropin (hCG) administration (levels >90th percentile and ≤ 90th percentile) (n = 491) and the third group on pregnancy outcome (positive/negative) (n = 376). The groups were matched for the other variables (stimulation protocol, dose of gonadotropin, duration of stimulation, antral follicle count, body mass index, basal follicle stimulating hormone (FSH), and E2 levels and day of hCG trigger) to prevent the possible contribution of those parameters to the results. Each group was matched for other variables (stimulation protocol, dose of gonadotrophin, duration of stimulation, antral follicle count, body mass index, basal FSH and E2 levels and day of hCG trigger) that may have affected the outcome, except for the parameter under investigation. Maternal age (P = 0.044,168 r = 0.418), oestrogen level on day of hCG administration (P = 0.001, r = 0.436) and pregnancy outcome (AUC = 0.65, P = 0.071) were found to be correlated with follicular oxidative status. The results obtained will help us to shield patients from possible situations that may cause oxidative stress and therefore adverse outcomes of an ICSI cycle.

Can follicular fluid 8-oxo-2’-deoxyguanosine predict the clinical outcomes in ICSI cycle among couples with normospermia male?

“…The inverse correlations of FF 8-OHdG with E2 and goodquality embryos were in agreement with earlier research; serum E2 levels were correlated with FF OS biomarkers that inversely affect ovarian responses [26].…”

“…The analysis confirmed a significantly high FF 8-OHdG level among non-pregnant women, in line with an earlier study that linked OS biomarkers to failed pregnancies [26].…”

The effect of adipose tissue StAR gene expression on ICSI among Polycystic Ovarian Syndrome cases and matched controls: a case-control study

“…In addition, OS serves as a prognostic biomarker in IVF success. Serum E2 levels were linked to follicular OS biomarkers that inversely impact ovarian response [31].…”