Factors Which Influence the Growth of Heat-treated Bacteria

Nelson, F. E.

doi:10.1128/jb.48.4.473-477.1944

Cited by 39 publications

(20 citation statements)

References 6 publications

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“…On the contrary, Roberts and Aldous (20) reported that recovery of UV-irradiated organisms was better on semisolid media than on conventional agar media. Nelson (18) showed that heat-treated spores of B. subtilis gave different counts on different agar media, and even the source of the agar has been reported to be important in the recovery process (13).…”

Section: Discussionmentioning

confidence: 99%

Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus

Abshire¹,

Bain²,

Williams³

1980

Appl Environ Microbiol

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A spore suspension model and a procedure for recovering ultraviolet (UV)-irradiated spores of Bacillus pumilus were investigated. A most-probable-number tube dilution method using double-strength Trypticase soy broth was found to be superior to the agar plate method for recovering optimal numbers of spores irradiated with sublethal doses of UV energy. Aqueous suspensions of B. pumilus survived UV doses up to 108,000 ergs/mm2 as determined by a most-probable-number recovery and estimation procedure. Resistance and stability data were consistent and reproducible, indicating the dependability of this method for recovering UV-damaged spores. The procedures used to collect information concerning resistance characteristics for two strains of B. pumilus are discussed.

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Section: Discussionmentioning

confidence: 99%

Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus

Abshire¹,

Bain²,

Williams³

1980

Appl Environ Microbiol

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“…In recent years, there have been several reports dealing with the revival of bacteria damaged by chemical and physical means (9,16). The composition and pH of the recovery medium and the temperature of incubation affect the recovery of bacteria damaged by phenol (9,12), heat (1,8,15,19; F. E. Nelson, Bacteriol. Proc., p. 40, 1956), and irradiation (2,18).…”

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confidence: 99%

Factors Influencing the Survival and Revival of Heat-treated Escherichia coli

Russell¹,

Harries²

1968

Applied Microbiology

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Maximal revival of heat-damaged Escherichia coli occurred in nutrient media containing 0.8 to 1.0% (w/v) of Difco yeast extract. Vitamins did not appear to be involved in the recovery process. The situation with amino acids was less clear-cut, and, although certain of these may be essential for revival, proof for this is as yet inconclusive. Replica plating, in which colonies (from cells which had survived a heating process) on a rich medium were replicated onto minimal agar, revealed that no auxotrophic mutants had been formed as a result of heat treatment. Ba cteria which were heated in 1 % (w/v) yeast extract were killed more slowly than those heated in water.

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“…The report of Curran and Evans (1937) indicates that organisms which survive treatment by physical or chemical agents are more fastidious in their nutritive requirements than unexposed cells. These observations were confirmed by Nelson (1943). Robinson and Hills (1959) observed that mild heating at 120 F (48.9 C) in the presence of 0.05 per cent sorbic acid greatly increased the shelf life of cider, peach slices, and fruit salads.…”

Section: Total Solidsmentioning

confidence: 61%